Microarray expression profile analysis of aberrant long non-coding RNAs in esophageal squamous cell carcinoma

Yao,Juan; Huang,Jun-Xing; Lin,Mei; Wu,Zheng-Dong; Yu,Hong; Wang,Peng-Cheng; Ye,Jun; Chen,Ping; Wu,Jing; Zhao,Guo-Jun

doi:10.3892/ijo.2016.3457

Microarray expression profile analysis of aberrant long non-coding RNAs in esophageal squamous cell carcinoma

Authors:
- Juan Yao
- Jun-Xing Huang
- Mei Lin
- Zheng-Dong Wu
- Hong Yu
- Peng-Cheng Wang
- Jun Ye
- Ping Chen
- Jing Wu
- Guo-Jun Zhao
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Affiliations: Department of Oncology, Taizhou People's Hospital Affiliated to Nantong and Jiangsu University, Taizhou, Jiangsu 225300, P.R. China, Department of Experiment Center, Taizhou People's Hospital Affiliated to Nantong and Jiangsu University, Taizhou, Jiangsu 225300, P.R. China, Department of Pathology, Taizhou People's Hospital Affiliated to Nantong and Jiangsu University, Taizhou, Jiangsu 225300, P.R. China, Department of Thoracic Surgery, Taizhou People's Hospital Affiliated to Nantong and Jiangsu University, Taizhou, Jiangsu 225300, P.R. China, Department of Oncology, The People's Hospital of Deyang, Chengdu University of TCM, Deyang, Sichuan 618000, P.R. China
Published online on: March 24, 2016 https://doi.org/10.3892/ijo.2016.3457
Pages: 2543-2557

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Abstract

Increasing evidence indicates that long non-coding RNA (lncRNA) plays an important role in tumorigenesis. However, the function and regulatory mechanism of lncRNAs are still unclear in esophageal squamous cell carcinoma (ESCC). To address this challenge, we screened lncRNAs expression profiles in 3 pairs of ESCC and matched non-cancerous tissues by microarray assay and identified the relationship between lncRNAs expression in ESCC tissue and clinicopathological characteristics and prognosis of patients with ESCC. We found 182 lncRNAs that were significantly differently expressed in ESCC tissues versus the matched non-cancerous tissues. Gene ontology and pathway analysis results suggested that the primary biological processes of these genes were involved in extracellular matrix, immune responses, cell differentiation and cell proliferation. Through cis and trans analyzing, we found 4 lncRNAs (ENST00000480669, NONHSAT104436, NONHSAT126998 and NONHSAT112918) may play important roles in tumorigenesis of ESCC. The four lncRNAs were checked in 73 patients with ESCC. The results showed that they mainly related to tumor metastasis. Kaplan-Meier survival analysis showed that high expression of NONHSAT104436, NONHSAT126998 and low expression of ENST00000480669 were related to poor 3-year overall survival (P=0.003, 0.032 and 0.040, respectively). Multivariate analysis showed that NONHSAT104436 was an independent prognostic factor (P=0.017). Thus we concluded that, lncRNAs showed differently expression patterns in ESCC versus matched non-cancerous tissues, and aberrantly expressed lncRNA may play important roles in ESCC development and progression. Interestingly, the overexpression of NONHSAT104436 was tightly correlated with distant metastasis and, poor survival rate, which might indicate that NONHSAT104436 might play a very important part in ESCC tumor progression.

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