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Article

EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling

  • Authors:
    • Xiu-Wu Pan
    • Lu Chen
    • Yi Hong
    • Dan-Feng Xu
    • Xi Liu
    • Lin Li
    • Yi Huang
    • Li-Ming Cui
    • Si-Shun Gan
    • Qi-Wei Yang
    • Hai Huang
    • Fa-Jun Qu
    • Jian-Qing Ye
    • Lin-Hui Wang
    • Xin-Gang Cui
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery of Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China, Duruo Biotechnologies Inc., Shanghai 200233, P.R. China, Department of Urinary Surgery of Ruijin Hospital, Shanghai Jiaotong University, Shanghai 200025, P.R. China, Department of Urinary Surgery of Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China
  • Pages: 2580-2590
    |
    Published online on: March 28, 2016
       https://doi.org/10.3892/ijo.2016.3459
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Abstract

There are no effective therapies for advanced renal cell carcinoma (RCC), except for VEGFR inhibitors with only ~50% response rate. To identify novel targets and biomarkers for RCC is of great importance in treating RCC. In this study, we observed that eukaryotic initiation factor 3d (EIF3D) expression was significantly increased in RCC compared with paracarcinoma tissue using immunohistochemistry staining and western blot analysis. Furthermore, bioinformatics meta-analysis using ONCOMINE microarray datasets showed that EIF3D mRNA expressions in CCRCC tissue specimens were significantly higher than that in normal tissue specimens. In addition, RCC tissue microarray demonstrated that elevated EIF3D expression was positively correlated with TNM stage and tumor size. EIF3D silencing in human 786-O and ACHN CCRCC cell lines by RNA interference demonstrated that EIF3D knockdown obviously inhibited cell proliferation and colony formation, caused G2/M arrest through downregulation of Cyclin B1 and Cdk1 and upregulation of p21, and induced apoptosis shown by sub-G1 accumulation and RARP cleavage. Moreover, correlation analysis using ONCOMINE microarray datasets indicated that increased EIF3D mRNA expression was positively correlated to PCNA, Cyclin B1 and CDK1 mRNA expression in RCC. Collectively, these results provide reasonable evidences that EIF3D may function as a potential proto-oncogene that participates in the occurrence and progression of RCC.
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View References

1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Hes O: International Society of Urological Pathology (ISUP) Vancouver Classification of Renal Neoplasia 2012. Cesk Patol. 50:137–141. 2014.(In Czech).

3 

Jiang Z, Chu PG, Woda BA, Liu Q, Balaji KC, Rock KL and Wu CL: Combination of quantitative IMP3 and tumor stage: A new system to predict metastasis for patients with localized renal cell carcinomas. Clin Cancer Res. 14:5579–5584. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Zhao J, Huang X, Sun F, Ma R, Wang H, Shao K, Zhu Y, Zhou W, Xu Z and Shen Z: Prognostic factors for overall survival with targeted therapy in Chinese patients with metastatic renal cell carcinoma. Can Urol Assoc J. 8:E821–E827. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Sciarra A, Gentile V, Salciccia S, Alfarone A and Di Silverio F: New anti-angiogenic targeted therapy in advanced renal cell carcinoma (RCC): Current status and future prospects. Rev Recent Clin Trials. 3:97–103. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Spilka R, Ernst C, Mehta AK and Haybaeck J: Eukaryotic translation initiation factors in cancer development and progression. Cancer Lett. 340:9–21. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Zhang Y, Yu JJ, Tian Y, Li ZZ, Zhang CY, Zhang SF, Cao LQ, Zhang Y, Qian CY, Zhang W, et al: eIF3a improve cisplatin sensitivity in ovarian cancer by regulating XPC and p27Kip1 translation. Oncotarget. 6:25441–25451. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Li Z, Lin S, Jiang T, Wang J, Lu H, Tang H, Teng M and Fan J: Overexpression of eIF3e is correlated with colon tumor development and poor prognosis. Int J Clin Exp Pathol. 7:6462–6474. 2014.PubMed/NCBI

9 

Lin Z, Xiong L and Lin Q: Knockdown of eIF3d inhibits cell proliferation through G2/M phase arrest in non-small cell lung cancer. Med Oncol. 32:1832015. View Article : Google Scholar : PubMed/NCBI

10 

Yu X, Zheng B and Chai R: Lentivirus-mediated knockdown of eukaryotic translation initiation factor 3 subunit D inhibits proliferation of HCT116 colon cancer cells. Biosci Rep. 34:e001612014. View Article : Google Scholar : PubMed/NCBI

11 

Ren M, Zhou C, Liang H, Wang X and Xu L: RNAi-mediated silencing of EIF3D alleviates proliferation and migration of glioma U251 and U87MG cells. Chem Biol Drug Des. 86:715–722. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Gao Y, Teng J, Hong Y, Qu F, Ren J, Li L, Pan X, Chen L, Yin L, Xu D, et al: The oncogenic role of EIF3D is associated with increased cell cycle progression and motility in prostate cancer. Med Oncol. 32:5182015. View Article : Google Scholar : PubMed/NCBI

13 

Li H, Zhou F, Wang H, Lin D, Chen G, Zuo X, Sun L, Zhang X and Yang S: Knockdown of EIF3D suppresses proliferation of human melanoma cells through G2/M phase arrest. Biotechnol Appl Biochem. 62:615–620. 2015. View Article : Google Scholar

14 

Silvera D, Arju R, Darvishian F, Levine PH, Zolfaghari L, Goldberg J, Hochman T, Formenti SC and Schneider RJ: Essential role for eIF4GI overexpression in the pathogenesis of inflammatory breast cancer. Nat Cell Biol. 11:903–908. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Dagher J, Dugay F, Rioux-Leclercq N, Verhoest G, Oger E, Bensalah K, Cabillic F, Jouan F, Kammerer-Jacquet SF, Fergelot P, et al: Cytoplasmic PAR-3 protein expression is associated with adverse prognostic factors in clear cell renal cell carcinoma and independently impacts survival. Hum Pathol. 45:1639–1646. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Cuddihy AR and O'Connell MJ: Cell-cycle responses to DNA damage in G2. Int Rev Cytol. 222:99–140. 2003. View Article : Google Scholar

17 

Hu X, Zhang Z, Liu T, Song L, Zhu J, Guo Z, Cai J and Yu R: Polypeptide fraction from Arca subcrenata induces apoptosis and G2/M phase arrest in HeLa cells via ROS-mediated MAPKs pathways. Evid Based Complement Alternat Med. 2015:9302492015. View Article : Google Scholar : PubMed/NCBI

18 

Su CC: Tanshinone IIA inhibits gastric carcinoma AGS cells through increasing p-p38, p-JNK and p53 but reducing p-ERK, CDC2 and cyclin B1 expression. Anticancer Res. 34:7097–7110. 2014.PubMed/NCBI

19 

Li JP, Yang YX, Liu QL, Pan ST, He ZX, Zhang X, Yang T, Chen XW, Wang D, Qiu JX, et al: The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells. Drug Des Devel Ther. 9:1627–1652. 2015.PubMed/NCBI

20 

Wang J, Wu A, Xu Y, Liu J and Qian X: M(2)-A induces apoptosis and G(2)-M arrest via inhibiting PI3K/Akt pathway in HL60 cells. Cancer Lett. 283:193–202. 2009. View Article : Google Scholar : PubMed/NCBI

21 

King KL and Cidlowski JA: Cell cycle regulation and apoptosis. Annu Rev Physiol. 60:601–617. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Boonstra J and Post JA: Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene. 337:1–13. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Michieli P, Chedid M, Lin D, Pierce JH, Mercer WE and Givol D: Induction of WAF1/CIP1 by a p53-independent pathway. Cancer Res. 54:3391–3395. 1994.PubMed/NCBI

24 

Abbas T and Dutta A: p21 in cancer: Intricate networks and multiple activities. Nat Rev Cancer. 9:400–414. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Wu CF and Efferth T: Miltirone induces G2/M cell cycle arrest and apoptosis in CCRF-CEM acute lymphoblastic leukemia cells. J Nat Prod. 78:1339–1347. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Abaza MS, Orabi KY, Al-Quattan E and Al-Attiyah RJ: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 15:462015. View Article : Google Scholar : PubMed/NCBI

27 

Piskunova TS, Yurova MN, Ovsyannikov AI, Semenchenko AV, Zabezhinski MA, Popovich IG, Wang ZQ and Anisimov VN: Deficiency in poly(ADP-ribose) polymerase-1 (PARP-1) accelerates aging and spontaneous carcinogenesis in mice. Curr Gerontol Geriatr Res. 2008:7541902008. View Article : Google Scholar

28 

Ghavami S, Hashemi M, Ande SR, Yeganeh B, Xiao W, Eshraghi M, Bus CJ, Kadkhoda K, Wiechec E, Halayko AJ, et al: Apoptosis and cancer: Mutations within caspase genes. J Med Genet. 46:497–510. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Ahn MY, Kim TH, Kwon SM, Yoon HE, Kim HS, Kim JI, Kim YC, Kang KW, Ahn SG and Yoon JH: 5-Nitro-5′-hydroxy-indirubin-3′-oxime (AGM130), an indirubin-3′-oxime derivative, inhibits tumor growth by inducing apoptosis against non-small cell lung cancer in vitro and in vivo. Eur J Pharm Sci. 79:122–131. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pan X, Chen L, Hong Y, Xu D, Liu X, Li L, Huang Y, Cui L, Gan S, Yang Q, Yang Q, et al: EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling. Int J Oncol 48: 2580-2590, 2016.
APA
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L. ... Cui, X. (2016). EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling. International Journal of Oncology, 48, 2580-2590. https://doi.org/10.3892/ijo.2016.3459
MLA
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L., Huang, Y., Cui, L., Gan, S., Yang, Q., Huang, H., Qu, F., Ye, J., Wang, L., Cui, X."EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling". International Journal of Oncology 48.6 (2016): 2580-2590.
Chicago
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L., Huang, Y., Cui, L., Gan, S., Yang, Q., Huang, H., Qu, F., Ye, J., Wang, L., Cui, X."EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling". International Journal of Oncology 48, no. 6 (2016): 2580-2590. https://doi.org/10.3892/ijo.2016.3459
Copy and paste a formatted citation
x
Spandidos Publications style
Pan X, Chen L, Hong Y, Xu D, Liu X, Li L, Huang Y, Cui L, Gan S, Yang Q, Yang Q, et al: EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling. Int J Oncol 48: 2580-2590, 2016.
APA
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L. ... Cui, X. (2016). EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling. International Journal of Oncology, 48, 2580-2590. https://doi.org/10.3892/ijo.2016.3459
MLA
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L., Huang, Y., Cui, L., Gan, S., Yang, Q., Huang, H., Qu, F., Ye, J., Wang, L., Cui, X."EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling". International Journal of Oncology 48.6 (2016): 2580-2590.
Chicago
Pan, X., Chen, L., Hong, Y., Xu, D., Liu, X., Li, L., Huang, Y., Cui, L., Gan, S., Yang, Q., Huang, H., Qu, F., Ye, J., Wang, L., Cui, X."EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling". International Journal of Oncology 48, no. 6 (2016): 2580-2590. https://doi.org/10.3892/ijo.2016.3459
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