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Article

Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG

  • Authors:
    • Antonella Orlando
    • Michele Linsalata
    • Francesco Russo
  • View Affiliations / Copyright

    Affiliations: Laboratory of Nutritional Pathophysiology, National Institute for Digestive Diseases I.R.C.C.S. ‘Saverio de Bellis’, Castellana Grotte, Ι-70013 Bari, Italy
  • Pages: 2629-2638
    |
    Published online on: March 29, 2016
       https://doi.org/10.3892/ijo.2016.3463
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Abstract

Vitamin K (VK), an essential nutrient associated with the clotting cascade, has also been demonstrated to have anticancer properties in various cancer cells including colon cancer cells. Also probiotics have gained interest as potential anticancer agents. Among them, Lactobacillus rhamnosus GG (L.GG) has been shown to inhibit cell proliferation and polyamine biosynthesis as well as to induce apoptosis in different human gastrointestinal cancer cells. Nevertheless, the exact mechanisms involved in these actions are not completely elucidated. Therefore, the aims of the present study were to evaluate in three differently graded human colon cancer cells (namely Caco-2, HT-29 and SW480) the effects of increasing VK1 concentrations, administered alone or in combination with viable L.GG, on the cell proliferation evaluated by MTT test, apoptosis investigated by Bax/Bcl-2 ratio and the percentage of the apoptotic cells, and the cell cycle evaluated by MUSE cell analyzer. Both VK1 and L.GG administered alone up to 72 h, caused inhibition of proliferation, induction of apoptosis and the cell cycle arrest in all the tested colon cancer cells. When VK1 and L.GG were co-administered, the addition of increasing VK1 concentrations potentiated the probiotic antiproliferative effect in a dose-dependent manner, being also related to the individual features of each cell line. The effect was more evident in Caco-2 and HT-29 cells compared to the less differentiated SW480. The enhanced antiproliferative efficacy due to co-administration of L.GG and VK1 could represent a suitable option in a functional food strategy for cancer growth inhibition and chemoprevention.
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Copy and paste a formatted citation
Spandidos Publications style
Orlando A, Linsalata M and Russo F: Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG. Int J Oncol 48: 2629-2638, 2016.
APA
Orlando, A., Linsalata, M., & Russo, F. (2016). Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG. International Journal of Oncology, 48, 2629-2638. https://doi.org/10.3892/ijo.2016.3463
MLA
Orlando, A., Linsalata, M., Russo, F."Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG". International Journal of Oncology 48.6 (2016): 2629-2638.
Chicago
Orlando, A., Linsalata, M., Russo, F."Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG". International Journal of Oncology 48, no. 6 (2016): 2629-2638. https://doi.org/10.3892/ijo.2016.3463
Copy and paste a formatted citation
x
Spandidos Publications style
Orlando A, Linsalata M and Russo F: Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG. Int J Oncol 48: 2629-2638, 2016.
APA
Orlando, A., Linsalata, M., & Russo, F. (2016). Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG. International Journal of Oncology, 48, 2629-2638. https://doi.org/10.3892/ijo.2016.3463
MLA
Orlando, A., Linsalata, M., Russo, F."Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG". International Journal of Oncology 48.6 (2016): 2629-2638.
Chicago
Orlando, A., Linsalata, M., Russo, F."Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG". International Journal of Oncology 48, no. 6 (2016): 2629-2638. https://doi.org/10.3892/ijo.2016.3463
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