Specific expression and methylation of SLIT1, SLIT2, SLIT3, and miR-218 in gastric cancer subtypes

  • Authors:
    • Mirang Kim
    • Jong-Hwan Kim
    • Su-Jin Baek
    • Seon-Young Kim
    • Yong Sung Kim
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  • Published online on: April 6, 2016     https://doi.org/10.3892/ijo.2016.3473
  • Pages: 2497-2507
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Abstract

SLIT has been suggested as a key regulator of cancer development and a promising therapeutic target for cancer treatment. Herein, we analyzed expression and methylation of SLIT1/SLIT2/SLIT3 in 11 gastric cancer cell lines, 96 paired gastric tumors and adjacent normal gastric tissues, and 250 gastric cancers provided by The Cancer Genome Atlas. Methylation of SLIT1/SLIT2/SLIT3 was found both in early gastric cancers, and in advanced gastric cancers. Even normal gastric tissue showed increased methylation of SLIT1 and SLIT3 that correlated with patient age. Furthermore, epigenetic inactivation of SLIT occurred in a gastric cancer subtype-dependent manner. SLIT2 and SLIT3 expression was reduced in Epstein-Barr virus-positive and microsatellite instability subtypes, but increased in the genomically stable subtype. Expression of miR‑218 correlated negatively with methylation of SLIT2 or SLIT3. These findings suggest that a molecular subtype-specific therapeutic strategy is needed for targeting SLITs and miR-218 in treatment of gastric cancer.
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June-2016
Volume 48 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim M, Kim J, Baek S, Kim S and Kim YS: Specific expression and methylation of SLIT1, SLIT2, SLIT3, and miR-218 in gastric cancer subtypes. Int J Oncol 48: 2497-2507, 2016
APA
Kim, M., Kim, J., Baek, S., Kim, S., & Kim, Y.S. (2016). Specific expression and methylation of SLIT1, SLIT2, SLIT3, and miR-218 in gastric cancer subtypes. International Journal of Oncology, 48, 2497-2507. https://doi.org/10.3892/ijo.2016.3473
MLA
Kim, M., Kim, J., Baek, S., Kim, S., Kim, Y. S."Specific expression and methylation of SLIT1, SLIT2, SLIT3, and miR-218 in gastric cancer subtypes". International Journal of Oncology 48.6 (2016): 2497-2507.
Chicago
Kim, M., Kim, J., Baek, S., Kim, S., Kim, Y. S."Specific expression and methylation of SLIT1, SLIT2, SLIT3, and miR-218 in gastric cancer subtypes". International Journal of Oncology 48, no. 6 (2016): 2497-2507. https://doi.org/10.3892/ijo.2016.3473