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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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June-2016 Volume 48 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy

  • Authors:
    • Xi Yang
    • Ashlee H. Tietje
    • Xianzhong Yu
    • Yanzhang Wei
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA, Division of Science, Southern Wesleyan University, Central, SC 29634, USA
  • Pages: 2381-2386
    |
    Published online on: April 7, 2016
       https://doi.org/10.3892/ijo.2016.3475
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Abstract

Whereas cancer immunotherapy with cytokines in recent research was demonstrated effective in activating immune response against tumor cells, one major obstacle with the use of these cytokines is their severe side effects when delivered systemically at high doses. Another challenge is that advanced tumor cells often evade immunosurveillance of the immune system as well as of the Fas-mediated apoptosis by various mechanisms. We report the design and preliminary evaluation of the antitumor activity of a novel fusion protein-mIL-12/FasTI, consisting of mouse interleukin-12 and the transmembrane and intracellular domains of mouse Fas. The fusion construct (pmIL-12/FasTI) was transfected into mouse lung carcinoma cell line TC-1. Stable cell clones expressing the fusion protein were established as assayed by RT-PCR and immunohistochemistry. ELISA and cell proliferation analyses demonstrated that NK cells were effectively activated by the fusion protein with increased IFN-γ production and cytotoxicity. Enhanced caspase-3 activity of the clones when co-cultured with NK cells indicated that apoptosis was induced through Fas/FasL signaling pathway. The preliminary results suggest a synergized anticancer activity of the fusion protein. It may represent a promising therapeutic agent for cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Tietje AH, Yu X and Wei Y: Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy. Int J Oncol 48: 2381-2386, 2016.
APA
Yang, X., Tietje, A.H., Yu, X., & Wei, Y. (2016). Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy. International Journal of Oncology, 48, 2381-2386. https://doi.org/10.3892/ijo.2016.3475
MLA
Yang, X., Tietje, A. H., Yu, X., Wei, Y."Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy". International Journal of Oncology 48.6 (2016): 2381-2386.
Chicago
Yang, X., Tietje, A. H., Yu, X., Wei, Y."Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy". International Journal of Oncology 48, no. 6 (2016): 2381-2386. https://doi.org/10.3892/ijo.2016.3475
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Tietje AH, Yu X and Wei Y: Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy. Int J Oncol 48: 2381-2386, 2016.
APA
Yang, X., Tietje, A.H., Yu, X., & Wei, Y. (2016). Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy. International Journal of Oncology, 48, 2381-2386. https://doi.org/10.3892/ijo.2016.3475
MLA
Yang, X., Tietje, A. H., Yu, X., Wei, Y."Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy". International Journal of Oncology 48.6 (2016): 2381-2386.
Chicago
Yang, X., Tietje, A. H., Yu, X., Wei, Y."Mouse interleukin-12/FasTI: A novel bi-functional fusion protein for cancer immuno/gene therapy". International Journal of Oncology 48, no. 6 (2016): 2381-2386. https://doi.org/10.3892/ijo.2016.3475
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