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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August-2016 Volume 49 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion

  • Authors:
    • Woo Hyun Park
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Medical School, Research Institute for Endocrine Sciences, Chonbuk National University, Jeonju 561-180, Republic of Korea
  • Pages: 785-792
    |
    Published online on: May 27, 2016
       https://doi.org/10.3892/ijo.2016.3543
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Abstract

Pyrogallol (PG) inhibits the growth of various cells via stimulating O2•--mediated death. This study investigated the effects of PG on cell death in human pulmonary fibroblast (HPF) cells in relation to reactive oxygen species (ROS) and glutathione (GSH) levels. PG inhibited the growth of HPF cells with an IC50 of ~50-100 µM at 24 h. PG induced a G1 phase arrest of the cell cycle and also triggered cell death accompanied by the loss of mitochondrial membrane potential (MMP; ∆ψm), Bcl-2 decrease, p53 increase and the activation of caspase-3. PG increased O2•- level in HPF cells and depleted GSH content in these cells. Z-VAD (a pan-caspase inhibitor) did not significantly change cell growth inhibition, death and MMP (∆ψm) loss in PG-treated HPF cells. N-acetylcysteine (NAC) attenuated growth inhibition, death and MMP (∆ψm) loss in PG-treated HPF cells and it decreased O2•- level in these cells as well. However, L-buthionine sulfoximine (BSO) strongly increased ROS level in PG-treated HPF cells and it intensified growth inhibition, cell death, MMP (∆ψm) loss and GSH depletion in these cells. In conclusion, PG-induced HPF cell death was closely related to increases in ROS level and GSH depletion.
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Copy and paste a formatted citation
Spandidos Publications style
Park WH: Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion. Int J Oncol 49: 785-792, 2016.
APA
Park, W.H. (2016). Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion. International Journal of Oncology, 49, 785-792. https://doi.org/10.3892/ijo.2016.3543
MLA
Park, W. H."Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion". International Journal of Oncology 49.2 (2016): 785-792.
Chicago
Park, W. H."Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion". International Journal of Oncology 49, no. 2 (2016): 785-792. https://doi.org/10.3892/ijo.2016.3543
Copy and paste a formatted citation
x
Spandidos Publications style
Park WH: Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion. Int J Oncol 49: 785-792, 2016.
APA
Park, W.H. (2016). Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion. International Journal of Oncology, 49, 785-792. https://doi.org/10.3892/ijo.2016.3543
MLA
Park, W. H."Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion". International Journal of Oncology 49.2 (2016): 785-792.
Chicago
Park, W. H."Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion". International Journal of Oncology 49, no. 2 (2016): 785-792. https://doi.org/10.3892/ijo.2016.3543
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