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Article

Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA

  • Authors:
    • Fangfang Jiang
    • Lijie Zhou
    • Changbo Wei
    • Wei Zhao
    • Dongsheng Yu
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, P.R. China
  • Pages: 709-719
    |
    Published online on: June 8, 2016
       https://doi.org/10.3892/ijo.2016.3570
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Abstract

As a new strategy, radio-gene therapy was widely used for the treatment of cancer patients in recent few years. Slug was involved in the radioresistance of various cancers and has been found to have an anti-apoptotic effect. This study aims to investigate whether the modulation of Slug expression by siRNA affects oral squamous cell carcinoma sensitivity to X-ray irradiation through upregulating PUMA. Two oral squamous cell carcinoma cell lines (HSC3 and HSC6) were transfected with small interfering RNA (siRNA) targeting Slug and subjected to radiotherapy in vitro. After transfection with Slug siRNA, both HSC3 and HSC6 cells showed relatively lower expression of Slug and higher expression of PUMA. The Slug siRNA transfected cells showed decreased survival and proliferation rates, an increased apoptosis rate and enhanced radiosensitivity to X-ray irradiation. Our results revealed that Slug siRNA transfection in combination with radiation increased the expression of PUMA, which contributed to radiosensitivity of oral squamous cell carcinoma cells. Thus, controlling the expression of Slug might contribute to enhance sensitivity of HSC3 and HSC6 cells toward X-ray irradiation in vitro by upregulating PUMA.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang F, Zhou L, Wei C, Zhao W and Yu D: Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA. Int J Oncol 49: 709-719, 2016.
APA
Jiang, F., Zhou, L., Wei, C., Zhao, W., & Yu, D. (2016). Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA. International Journal of Oncology, 49, 709-719. https://doi.org/10.3892/ijo.2016.3570
MLA
Jiang, F., Zhou, L., Wei, C., Zhao, W., Yu, D."Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA". International Journal of Oncology 49.2 (2016): 709-719.
Chicago
Jiang, F., Zhou, L., Wei, C., Zhao, W., Yu, D."Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA". International Journal of Oncology 49, no. 2 (2016): 709-719. https://doi.org/10.3892/ijo.2016.3570
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang F, Zhou L, Wei C, Zhao W and Yu D: Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA. Int J Oncol 49: 709-719, 2016.
APA
Jiang, F., Zhou, L., Wei, C., Zhao, W., & Yu, D. (2016). Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA. International Journal of Oncology, 49, 709-719. https://doi.org/10.3892/ijo.2016.3570
MLA
Jiang, F., Zhou, L., Wei, C., Zhao, W., Yu, D."Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA". International Journal of Oncology 49.2 (2016): 709-719.
Chicago
Jiang, F., Zhou, L., Wei, C., Zhao, W., Yu, D."Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA". International Journal of Oncology 49, no. 2 (2016): 709-719. https://doi.org/10.3892/ijo.2016.3570
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