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International Journal of Oncology
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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells

  • Authors:
    • Kan Fang
    • Peifeng Liu
    • Suyan Dong
    • Yanjie Guo
    • Xinxin Cui
    • Xiaoying Zhu
    • Xuan Li
    • Lianghan Jiang
    • Te Liu
    • Yuncheng Wu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, P.R. China, Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, P.R. China
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 509-518
    |
    Published online on: June 8, 2016
       https://doi.org/10.3892/ijo.2016.3571
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Abstract

Glioma stem cells (GSCs) are a special subpopulation of glioma cells that are key to the sensitivity of tumors to treatments and to the possibility of tumor recurrence. Identifying new strategies that inhibit the growth of GSCs are therefore important for developing novel therapies for glioblastoma multiforme (GBM). In this study, CD133+ human glioma stem cells were isolated and cultured. Magnetic nanoparticles were used to mediate the expression of siRNAs targeting the HOTAIR (si-HOTAIR) sequence in human gliomas. Effect of downregulation of HOTAIR expression on proliferation, invasion and in vivo tumorigenicity of human GSCs and underlying molecular mechanisms were further evaluated. The results of the MTT assay and flow cytometric analysis showed that downregulation of HOTAIR expression inhibited cell proliferation and induced cell cycle arrest. Transwell assays demonstrated that downregulation of HOTAIR expression resulted in a decrease in the invasive capability of GSCs. Moreover, magnetic nanoparticle-mediated low expression of HOTAIR effectively reduced the tumorigenic capacity of glioma stem cells in vivo. In addition, the results of qRT-PCR and western blot analysis demonstrated that downregulation of HOTAIR expression significantly increased the expression of PDCD4 in GSCs, in addition to reducing the expression of CCND1 and CDK4. An in-depth mechanistic analysis showed that downregulation of HOTAIR expression reduced the recruitment of downstream molecules, EZH2 and LSD1, thereby activating the expression of PDCD4 at the transcriptional level. In conclusion, downregulation of HOTAIR expression effectively promoted the expression of PDCD4, thereby inhibiting the proliferation, invasion and in vivo tumorigenicity of human GSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Fang K, Liu P, Dong S, Guo Y, Cui X, Zhu X, Li X, Jiang L, Liu T, Wu Y, Wu Y, et al: Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells. Int J Oncol 49: 509-518, 2016.
APA
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X. ... Wu, Y. (2016). Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells. International Journal of Oncology, 49, 509-518. https://doi.org/10.3892/ijo.2016.3571
MLA
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X., Li, X., Jiang, L., Liu, T., Wu, Y."Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells". International Journal of Oncology 49.2 (2016): 509-518.
Chicago
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X., Li, X., Jiang, L., Liu, T., Wu, Y."Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells". International Journal of Oncology 49, no. 2 (2016): 509-518. https://doi.org/10.3892/ijo.2016.3571
Copy and paste a formatted citation
x
Spandidos Publications style
Fang K, Liu P, Dong S, Guo Y, Cui X, Zhu X, Li X, Jiang L, Liu T, Wu Y, Wu Y, et al: Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells. Int J Oncol 49: 509-518, 2016.
APA
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X. ... Wu, Y. (2016). Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells. International Journal of Oncology, 49, 509-518. https://doi.org/10.3892/ijo.2016.3571
MLA
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X., Li, X., Jiang, L., Liu, T., Wu, Y."Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells". International Journal of Oncology 49.2 (2016): 509-518.
Chicago
Fang, K., Liu, P., Dong, S., Guo, Y., Cui, X., Zhu, X., Li, X., Jiang, L., Liu, T., Wu, Y."Magnetofection based on superparamagnetic iron oxide nanoparticle-mediated low lncRNA HOTAIR expression decreases the proliferation and invasion of glioma stem cells". International Journal of Oncology 49, no. 2 (2016): 509-518. https://doi.org/10.3892/ijo.2016.3571
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