Enhanced expression of semaphorin 3E is involved in the gastric cancer development

Maejima,Ryuhei; Tamai,Keiichi; Shiroki,Takeharu; Yokoyama,Misa; Shibuya,Rie; Nakamura,Mao; Yamaguchi,Kazunori; Abue,Makoto; Oikawa,Tomoyuki; Noguchi,Tetsuya; Miura,Koh; Fujiya,Tsuneaki; Sato,Ikuro; Iijima,Katsunori; Shimosegawa,Tooru; Tanaka,Nobuyuki; Satoh,Kennichi

doi:10.3892/ijo.2016.3593

September-2016 Volume 49 Issue 3

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September-2016 Volume 49 Issue 3

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Enhanced expression of semaphorin 3E is involved in the gastric cancer development

Authors:
- Ryuhei Maejima
- Keiichi Tamai
- Takeharu Shiroki
- Misa Yokoyama
- Rie Shibuya
- Mao Nakamura
- Kazunori Yamaguchi
- Makoto Abue
- Tomoyuki Oikawa
- Tetsuya Noguchi
- Koh Miura
- Tsuneaki Fujiya
- Ikuro Sato
- Katsunori Iijima
- Tooru Shimosegawa
- Nobuyuki Tanaka
- Kennichi Satoh
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Affiliations: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan, Division of Molecular and Cellular Oncology, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan, Department of Gastroenterology, Miyagi Cancer Center, Natori, Miyagi, Japan, Department of Gastroenterological Surgery, Miyagi Cancer Center, Natori, Miyagi, Japan, Department of Pathology, Miyagi Cancer Center, Natori, Miyagi, Japan, Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan, Division of Cancer Biology and Therapeutics, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan

Copyright: © Maejima et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 887-894
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Published online on: June 30, 2016

https://doi.org/10.3892/ijo.2016.3593
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Abstract

Semaphorins and their receptors are abnormally expressed in various cancers, but little is known about the expression and function of semaphorin 3E (SEMA3E) and its receptor, plexin D1 (PLXND1), in gastric cancer development or metastasis. We evaluated SEMA3E and PLXND1 expression by quantitative RT-PCR in gastric tissues from 62 patients who underwent gastrectomy and analyzed the correlation between their expression and clinicopathological variables. To assess the function of SEMA3E, we generated human gastric cancer cell lines with suppressed or increased SEMA3E expression. The expression level of SEMA3E, but not PLXND1, was correlated with lymph node involvement and metastatic progression in gastric cancer. A significant association was observed between a high level of SEMA3E expression and poor differentiation or poor survival in the intestinal type of gastric cancer. SEMA3E knockdown in gastric cancer cells attenuated cell proliferation and metastatic ability in vitro and in vivo. Moreover, SEMA3E caused cell proliferation and anchorage-independent cell growth in the intestinal type of gastric cancer. These results suggested that SEMA3E is likely to be involved in the development of gastric cancer and might also be a therapeutic target for its treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Enhanced expression of semaphorin 3E is involved in the gastric cancer development

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