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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy

  • Authors:
    • Jung Seok Kim
    • Seong Jae Kang
    • Hwa Yeon Jeong
    • Min Woo Kim
    • Sang Il Park
    • Yeon Kyung Lee
    • Hong Sung Kim
    • Keun Sik Kim
    • Yong Serk Park
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Laboratory Science, Yonsei University, Wonju, Republic of Korea, Department of Biomedical Laboratory Science, Korea Nazarene University, Cheonan, Republic of Korea, Department of Biomedical Laboratory Science, Konyang University, Daejeon, Republic of Korea
  • Pages: 1130-1138
    |
    Published online on: July 7, 2016
       https://doi.org/10.3892/ijo.2016.3619
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Abstract

Tumor-directed gene delivery is of major interest in the field of cancer gene therapy. Varied functionalizations of non-viral vectors have been suggested to enhance tumor targetability. In the present study, we prepared two different types of anti-EGF receptor (EGFR) immunonanoparticles containing pDNA, neutrally charged liposomes and cationic lipoplexes, for tumor-directed transfection of cancer therapeutic genes. Even though both anti-EGFR immunonanoparticles had a high binding affinity to the EGFR-positive cancer cells, the anti-EGFR immunolipoplex formulation exhibited approximately 100-fold higher transfection to the target cells than anti-EGFR immunoliposomes. The lipoplex formulation also showed a higher transfection to SK-OV-3 tumor xenografts in mice. Thus, IL12 and/or salmosin genes were loaded in the anti-EGFR immunolipoplexes and intravenously administered to mice carrying SK-OV-3 tumors. Co-transfection of IL12 and salmosin genes using anti-EGFR immunolipoplexes significantly reduced tumor growth and pulmonary metastasis. Furthermore, combinatorial treatment with doxorubicin synergistically inhibited tumor growth. These results suggest that anti-EGFR immunolipoplexes containing pDNA encoding therapeutic genes could be utilized as a gene-transfer modality for cancer gene therapy.
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1 

Ferreira GN, Monteiro GA, Prazeres DM and Cabral JM: Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications. Trends Biotechnol. 18:380–388. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Knipe JM, Peters JT and Peppas NA: Theranostic agents for intracellular gene delivery with spatiotemporal imaging. Nano Today. 8:21–38. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Nam HY, Park JH, Kim K, Kwon IC and Jeong SY: Lipid-based emulsion system as non-viral gene carriers. Arch Pharm Res. 32:639–646. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Wang Y, Miao L, Satterlee A and Huang L: Delivery of oligonucleotides with lipid nanoparticles. Adv Drug Deliv Rev. 87:68–80. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Zhao Y and Huang L: Lipid nanoparticles for gene delivery. Adv Genet. 88:13–36. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Malone RW, Felgner PL and Verma IM: Cationic liposome-mediated RNA transfection. Proc Natl Acad Sci USA. 86:6077–6081. 1989. View Article : Google Scholar : PubMed/NCBI

7 

Schroeder A, Levins CG, Cortez C, Langer R and Anderson DG: Lipid-based nanotherapeutics for siRNA delivery. J Intern Med. 267:9–21. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Fehring V, Schaeper U, Ahrens K, Santel A, Keil O, Eisermann M, Giese K and Kaufmann J: Delivery of therapeutic siRNA to the lung endothelium via novel Lipoplex formulation DACC. Mol Ther. 22:811–820. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Bergen JM, Park IK, Horner PJ and Pun SH: Nonviral approaches for neuronal delivery of nucleic acids. Pharm Res. 25:983–998. 2008. View Article : Google Scholar :

10 

Jokerst JV, Lobovkina T, Zare RN and Gambhir SS: Nanoparticle PEGylation for imaging and therapy. Nanomedicine (Lond). 6:715–728. 2011. View Article : Google Scholar

11 

Li SD and Huang L: Nanoparticles evading the reticuloendothelial system: Role of the supported bilayer. Biochim Biophys Acta. 1788:2259–2266. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Huang L and Liu Y: In vivo delivery of RNAi with lipid-based nanoparticles. Annu Rev Biomed Eng. 13:507–530. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Majzoub RN, Chan CL, Ewert KK, Silva BF, Liang KS, Jacovetty EL, Carragher B, Potter CS and Safinya CR: Uptake and transfection efficiency of PEGylated cationic liposome-DNA complexes with and without RGD-tagging. Biomaterials. 35:4996–5005. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Lee YK, Lee TS, Song IH, Jeong HY, Kang SJ, Kim MW, Ryu SH, Jung IH, Kim JS and Park YS: Inhibition of pulmonary cancer progression by epidermal growth factor receptor-targeted transfection with Bcl-2 and survivin siRNAs. Cancer Gene Ther. 22:335–343. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Baek SE, Lee KH, Park YS, Oh DK, Oh S, Kim KS and Kim DE: RNA aptamer-conjugated liposome as an efficient anticancer drug delivery vehicle targeting cancer cells in vivo. J Control Release. 196:234–242. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Yoo MK, Kang SK, Choi JH, Park IK, Na HS, Lee HC, Kim EB, Lee NK, Nah JW, Choi YJ, et al: Targeted delivery of chitosan nanoparticles to Peyer’s patch using M cell-homing peptide selected by phage display technique. Biomaterials. 31:7738–7747. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Portnoy E, Lecht S, Lazarovici P, Danino D and Magdassi S: Cetuximab-labeled liposomes containing near-infrared probe for in vivo imaging. Nanomedicine. 7:480–488. 2011.PubMed/NCBI

18 

Sato JD, Kawamoto T, Le AD, Mendelsohn J, Polikoff J and Sato GH: Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol Biol Med. 1:511–529. 1983.PubMed/NCBI

19 

Kang IC, Chung KH, Lee SJ, Yun Y, Moon HM and Kim DS: Purification and molecular cloning of a platelet aggregation inhibitor from the snake (Agkistrodon halys brevicaudus) venom. Thromb Res. 91:65–73. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Kang IC, Lee YD and Kim DS: A novel disintegrin salmosin inhibits tumor angiogenesis. Cancer Res. 59:3754–3760. 1999.PubMed/NCBI

21 

Nishitani M, Sakai T, Kanayama H, Himeno K and Kagawa S: Cytokine gene therapy for cancer with naked DNA. Mol Urol. 4:47–50. 2000. View Article : Google Scholar

22 

Park YS, Kim KS, Lee YK, Kim JS, Baek JY and Huang L: Inhibition of TC-1 tumor progression by cotransfection of Saxatilin and IL-12 genes mediated by lipofection or electroporation. Oncol Res. 18:203–212. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Goodwin T and Huang L: Nonviral vectors: We have come a long way. Adv Genet. 88:1–12. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Tam YY, Chen S and Cullis PR: Advances in lipid nanoparticles for siRNA delivery. Pharmaceutics. 5:498–507. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Whitehead KA, Dorkin JR, Vegas AJ, Chang PH, Veiseh O, Matthews J, Fenton OS, Zhang Y, Olejnik KT, Yesilyurt V, et al: Degradable lipid nanoparticles with predictable in vivo siRNA delivery activity. Nat Commun. 5:42772014. View Article : Google Scholar : PubMed/NCBI

26 

Zhao Y, Zhang S, Zhang Y, Cui S, Chen H, Zhi D, Zhen Y, Zhang S and Huang L: Tri-peptide cationic lipids for gene delivery. J Mater Chem B Mater Biol Med. 3:119–126. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Huang X, Schwind S, Yu B, Santhanam R, Wang H, Hoellerbauer P, Mims A, Klisovic R, Walker AR, Chan KK, et al: Targeted delivery of microRNA-29b by transferrin-conjugated anionic lipopolyplex nanoparticles: A novel therapeutic strategy in acute myeloid leukemia. Clin Cancer Res. 19:2355–2367. 2013.PubMed/NCBI

28 

Li SD and Huang L: Stealth nanoparticles: High density but sheddable PEG is a key for tumor targeting. J Control Release. 145:178–181. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Zhao YN, Qureshi F, Zhang SB, Cui SH, Wang B, Chen HY, Lv HT, Zhang SF and Huang L: Novel gemini cationic lipids with carbamate groups for gene delivery. J Mater Chem B Mater Biol Med. 2:2920–2928. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Askarian S, Abnous K, Taghavi S, Oskuee RK and Ramezani M: Cellular delivery of shRNA using aptamer-conjugated PLL-alkyl-PEI nanoparticles. Colloids Surf B Biointerfaces. 136:355–364. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Shi C, Gao F, Gao X and Liu Y: A novel anti-VEGF165 monoclonal antibody-conjugated liposomal nanocarrier system: Physical characterization and cellular uptake evaluation in vitro and in vivo. Biomed Pharmacother. 69:191–200. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Xie X, Yang Y, Yang Y and Mei X: Photolabile-caged peptide-conjugated liposomes for siRNA delivery. J Drug Target. 23:789–799. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Eliaz RE and Szoka FC Jr: Liposome-encapsulated doxorubicin targeted to CD44: A strategy to kill CD44-overexpressing tumor cells. Cancer Res. 61:2592–2601. 2001.PubMed/NCBI

34 

Kim HS, Song IH, Kim JC, Kim EJ, Jang DO and Park YS: In vitro and in vivo gene-transferring characteristics of novel cationic lipids, DMKD (O,O’-dimyristyl-N-lysyl aspartate) and DMKE (O,O’-dimyristyl-N-lysyl glutamate). J Control Release. 115:234–241. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Dokka S, Toledo D, Shi X, Castranova V and Rojanasakul Y: Oxygen radical-mediated pulmonary toxicity induced by some cationic liposomes. Pharm Res. 17:521–525. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Hafez IM, Maurer N and Cullis PR: On the mechanism whereby cationic lipids promote intracellular delivery of polynucleic acids. Gene Ther. 8:1188–1196. 2001. View Article : Google Scholar : PubMed/NCBI

37 

Kim SI, Kim KS, Kim HS, Kim DS, Jang Y, Chung KH and Park YS: Inhibitory effect of the salmosin gene transferred by cationic liposomes on the progression of B16BL6 tumors. Cancer Res. 63:6458–6462. 2003.PubMed/NCBI

38 

Kim HS, Jeong HY, Lee YK, Kim KS and Park YS: Synergistic antitumoral effect of IL-12 gene cotransfected with antiangiogenic genes for angiostatin, endostatin, and saxatilin. Oncol Res. 21:209–216. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Kim JS, Kang SJ, Jeong HY, Kim MW, Park SI, Lee YK, Kim HS, Kim KS and Park YS: Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy. Int J Oncol 49: 1130-1138, 2016.
APA
Kim, J.S., Kang, S.J., Jeong, H.Y., Kim, M.W., Park, S.I., Lee, Y.K. ... Park, Y.S. (2016). Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy. International Journal of Oncology, 49, 1130-1138. https://doi.org/10.3892/ijo.2016.3619
MLA
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49.3 (2016): 1130-1138.
Chicago
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49, no. 3 (2016): 1130-1138. https://doi.org/10.3892/ijo.2016.3619
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JS, Kang SJ, Jeong HY, Kim MW, Park SI, Lee YK, Kim HS, Kim KS and Park YS: Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy. Int J Oncol 49: 1130-1138, 2016.
APA
Kim, J.S., Kang, S.J., Jeong, H.Y., Kim, M.W., Park, S.I., Lee, Y.K. ... Park, Y.S. (2016). Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy. International Journal of Oncology, 49, 1130-1138. https://doi.org/10.3892/ijo.2016.3619
MLA
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49.3 (2016): 1130-1138.
Chicago
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49, no. 3 (2016): 1130-1138. https://doi.org/10.3892/ijo.2016.3619
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