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Article

Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer

  • Authors:
    • Zhifang Gui
    • Feng Luo
    • Yayang Yang
    • Can Shen
    • Shuquan Li
    • Jian Xu
  • View Affiliations / Copyright

    Affiliations: Medical Technology College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 111-120
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    Published online on: November 18, 2016
       https://doi.org/10.3892/ijo.2016.3772
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Abstract

The relationship among oridonin, miR-200b-3p and pancreatic cancer on epithelial-to-mesenchymal transition (EMT) was investigated for the molecular mechanism or signaling pathways on the migration in pancreatic cancer. BxPC-3 and PANC-1 cells were cultivated and the IC50 of oridonin in BxPC-3 and PANC-1 cells were obtained by the CCK-8 array. The expression of miR‑200b-3p was verified by using real-time PCR and its target gene was predicted. BxPC-3 and PANC-1 cells were treated with oridonin or transfected by miR-200b-3p, those cells were used for western blot assay, Transwell assay, ELISA, immunofluorescence staining, tumorigenesis assay in nude mice and immunohistochemical assay to verify the effects of oridonin or miR-200b-3p on pancreatic cancer. We found that oridonin inhibited the proliferation of BxPC-3 and PANC-1 cells in a dose-dependent manner. miR-200b-3p was downregulated by oridonin in BxPC-3 and PANC-1 cells. ZEB1 was a target gene for miR-200b-3p. Oridonin or overexpression of miR‑200b-3p can inhibit the cell migration in BxPC-3 and PANC-1 cells. miR-200b-3p can inhibit the EMT and oridonin can inhibit the expression of ZEB1, N-cadherin and fibronectin but not increase the expression of E-cadherin, while the cell adhesion molecules ICAM-1 and VCAM-1 were decreased by oridonin in BxPC-3 and PANC-1 cells and the cytoskeleton was altered by oridonin in PANC-1 cells compared with the control. In summary, the results demonstrate that miR‑200b-3p was able to inhibit the EMT of human pancreatic cancer in vivo and in vitro by targeted ZEB1. In vitro, oridonin had a certain effect on the migration in BxPC-3 and PANC-1 cells, but not though type III EMT by miR-200-3p/ZEB1 axis, and may be related to type Ⅱ EMT, tumor microenvironment or altering the cytoskeleton. In vivo, oridonin inhibited the cancer migration in the nude mouse model though inhibiting EMT.
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Copy and paste a formatted citation
Spandidos Publications style
Gui Z, Luo F, Yang Y, Shen C, Li S and Xu J: Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer. Int J Oncol 50: 111-120, 2017.
APA
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., & Xu, J. (2017). Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer. International Journal of Oncology, 50, 111-120. https://doi.org/10.3892/ijo.2016.3772
MLA
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., Xu, J."Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer". International Journal of Oncology 50.1 (2017): 111-120.
Chicago
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., Xu, J."Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer". International Journal of Oncology 50, no. 1 (2017): 111-120. https://doi.org/10.3892/ijo.2016.3772
Copy and paste a formatted citation
x
Spandidos Publications style
Gui Z, Luo F, Yang Y, Shen C, Li S and Xu J: Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer. Int J Oncol 50: 111-120, 2017.
APA
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., & Xu, J. (2017). Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer. International Journal of Oncology, 50, 111-120. https://doi.org/10.3892/ijo.2016.3772
MLA
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., Xu, J."Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer". International Journal of Oncology 50.1 (2017): 111-120.
Chicago
Gui, Z., Luo, F., Yang, Y., Shen, C., Li, S., Xu, J."Oridonin inhibition and miR‑200b‑3p/ZEB1 axis in human pancreatic cancer". International Journal of Oncology 50, no. 1 (2017): 111-120. https://doi.org/10.3892/ijo.2016.3772
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