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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma

  • Authors:
    • Miao Yu
    • Huanzhou Xue
    • Yadong Wang
    • Quan Shen
    • Qingfeng Jiang
    • Xiao Zhang
    • Ke Li
    • Meng Jia
    • Jiangkun Jia
    • Jian Xu
    • Yuwei Tian
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, People's Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China
  • Pages: 975-983
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/ijo.2017.3852
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Abstract

MicroRNAs (miRNAs) have been reported to play critical roles in tumor progression including hepatocellular carcinoma (HCC). Thus, the underlying mechanisms need further investigation. Previous study reported that loss of miR-345 expression indicated a poor prognosis of HCC patients. This study evaluated whether loss of miR-345 could promote the tumor metastasis and epithelial-mesenchymal-transition (EMT) of HCC by targeting interferon regulatory factor 1 (IRF1)-mediated mTOR/STAT3/AKT signaling. Underexpression of miR-345 was identified in 65 cases of human HCC compared to matched tumor-adjacent tissues by qRT-PCR. Moreover, we found that reduced expression of mi-345 was observed in HCC cell lines. The restoration of miR-345 inhibited cell migration and invasion in HCCLM3 cells, while its loss facilitated the cell mobility of HepG2 cells. Furthermore, miR-345 over­expression reduced lung metastases of HCC cells in nude mice. Notably, miR-345 overexpression prohibited, while its knockdown enhanced the EMT process of HCC cell lines in vitro. Bioinformatics software predicted that IRF1 was a direct target of miR-345. We then observed the negative regulation of miR-345 on IRF1 protein expression and the direct binding between them was further verified by dual-luciferase assays in HCC cells. In addition, over­expression of IRF1 mRNA was inversely correlated with the level of miR-345 in HCC specimens. Restoration of IRF1 resulted in promoted EMT and cell mobility in miR-345 overexpressing HCCLM3 cells. It was found that mTOR/STAT3/AKT pathway and its downstream targets including Slug, Snail and Twist may be involved in IRF1 mediated EMT process. In conclusion, miR-345 acts as an inhibitor of EMT process in HCC cells by targeting IRF1 and this study highlights the potential effects of miR-345 on prognosis and treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yu M, Xue H, Wang Y, Shen Q, Jiang Q, Zhang X, Li K, Jia M, Jia J, Xu J, Xu J, et al: miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma. Int J Oncol 50: 975-983, 2017.
APA
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X. ... Tian, Y. (2017). miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma. International Journal of Oncology, 50, 975-983. https://doi.org/10.3892/ijo.2017.3852
MLA
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X., Li, K., Jia, M., Jia, J., Xu, J., Tian, Y."miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma". International Journal of Oncology 50.3 (2017): 975-983.
Chicago
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X., Li, K., Jia, M., Jia, J., Xu, J., Tian, Y."miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma". International Journal of Oncology 50, no. 3 (2017): 975-983. https://doi.org/10.3892/ijo.2017.3852
Copy and paste a formatted citation
x
Spandidos Publications style
Yu M, Xue H, Wang Y, Shen Q, Jiang Q, Zhang X, Li K, Jia M, Jia J, Xu J, Xu J, et al: miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma. Int J Oncol 50: 975-983, 2017.
APA
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X. ... Tian, Y. (2017). miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma. International Journal of Oncology, 50, 975-983. https://doi.org/10.3892/ijo.2017.3852
MLA
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X., Li, K., Jia, M., Jia, J., Xu, J., Tian, Y."miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma". International Journal of Oncology 50.3 (2017): 975-983.
Chicago
Yu, M., Xue, H., Wang, Y., Shen, Q., Jiang, Q., Zhang, X., Li, K., Jia, M., Jia, J., Xu, J., Tian, Y."miR-345 inhibits tumor metastasis and EMT by targeting IRF1-mediated mTOR/STAT3/AKT pathway in hepatocellular carcinoma". International Journal of Oncology 50, no. 3 (2017): 975-983. https://doi.org/10.3892/ijo.2017.3852
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