Silencing Snail suppresses tumor cell proliferation and invasion by reversing epithelial-to-mesenchymal transition and arresting G2/M phase in non-small cell lung cancer

  • Authors:
    • Xueying Yang
    • Mengmeng Han
    • Haibo Han
    • Bingjing Wang
    • Sheng Li
    • Zhiqian Zhang
    • Wei Zhao
  • View Affiliations

  • Published online on: February 21, 2017     https://doi.org/10.3892/ijo.2017.3888
  • Pages: 1251-1260
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Abstract

Epithelial-to-mesenchymal transition (EMT) is essential for tumor invasion and metastasis. Snail has been proven to be a key regulator of EMT. Several studies have shown compelling evidence that Snail is also an important regulator of tumor growth and aggression; however, the role of Snail in the cell cycle has not been clarified. We decreased Snail expression by siRNA transfection and lentiviral‑mediated RNAi, to explore the effect of silencing Snail on the tumorigenicity and migration of lung carcinoma (lung cancer) cells. The results showed that silencing Snail conferred significant anti-proliferative activity and inhibited cell migration, tumor growth and metastasis both in vitro and in vivo. To understand the mechanism of these effects, we further investigated correlations among Snail expression, EMT and cell cycle. Significantly, Snail knockdown reversed EMT processes in lung cancer cells. Furthermore, the cyclin-dependent kinase inhibitor P21 was upregulated after silencing Snail. P21 upregulation manifested its tumor suppressor effects and arrested cells in the G2/M phase, not the G1/S phase following Snail depletion in lung cancer cells. These data suggest that silencing Snail decreases the malignant behaviors of lung cancer cells by reversing EMT processes and causing cell cycle defects.
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April-2017
Volume 50 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yang X, Han M, Han H, Wang B, Li S, Zhang Z and Zhao W: Silencing Snail suppresses tumor cell proliferation and invasion by reversing epithelial-to-mesenchymal transition and arresting G2/M phase in non-small cell lung cancer. Int J Oncol 50: 1251-1260, 2017
APA
Yang, X., Han, M., Han, H., Wang, B., Li, S., Zhang, Z., & Zhao, W. (2017). Silencing Snail suppresses tumor cell proliferation and invasion by reversing epithelial-to-mesenchymal transition and arresting G2/M phase in non-small cell lung cancer. International Journal of Oncology, 50, 1251-1260. https://doi.org/10.3892/ijo.2017.3888
MLA
Yang, X., Han, M., Han, H., Wang, B., Li, S., Zhang, Z., Zhao, W."Silencing Snail suppresses tumor cell proliferation and invasion by reversing epithelial-to-mesenchymal transition and arresting G2/M phase in non-small cell lung cancer". International Journal of Oncology 50.4 (2017): 1251-1260.
Chicago
Yang, X., Han, M., Han, H., Wang, B., Li, S., Zhang, Z., Zhao, W."Silencing Snail suppresses tumor cell proliferation and invasion by reversing epithelial-to-mesenchymal transition and arresting G2/M phase in non-small cell lung cancer". International Journal of Oncology 50, no. 4 (2017): 1251-1260. https://doi.org/10.3892/ijo.2017.3888