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Article

miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition

  • Authors:
    • Ke Su
    • Chun-Fang Wang
    • Ying Zhang
    • Yu-Jie Cai
    • Yan-Yan Zhang
    • Qian Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1211-1220
    |
    Published online on: March 7, 2017
       https://doi.org/10.3892/ijo.2017.3897
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Abstract

Cervical cancer is considered as a gynecological malignancy accompanied with high rates of mortality across the world. Effective diagnostic, prognostic markers as well as therapeutic targets are necessary to be explored. The p27 and PTEN are known to modulate tumor cell growth and proliferation. However, the molecular mechanisms modulating these genes have not completely been elucidated. In our study, real-time (RT) quantitative PCR indicated that miR‑940 levels were upregulated in human cervical cancer tissue samples and cell lines. Overexpression of miR‑940 could reduce overall survival in patients. Ectopic miR‑940 accelerated cervical cancer cell growth, proliferation and cell cycle arrest in vitro as well as tumor formation in vivo. p27 and PTEN were evidenced as direct targets for miR‑940 and inhibition of p27 and PTEN recovered the suppressive function of miR‑940-silenced cell towards to proliferation and tumorigenicity in cervical cancer cells. In addition, miR‑940 expression was inversely associated with p27 and PTEN expression levels and actively with cyclin D1 in cervical cancer specimens. The results from our study demonstrated that miR‑940 regulated p27 and PTEN post-transcriptionally and might play a significant role in cervical cancer development and progression. Thus, miR‑940 might provide a potential value as therapeutic target for cervical cancer treatment in future.
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View References

1 

World Health Organization: International Agency for Research on Cancer: Cervical Cancer - Estimated Incidence. Mortality and Prevalence Worldwide in 2012. http://globocan.iarc.fr/old/FactSheets/cancers/cervix-new.asp. Accession date: 2016-9-10.

2 

National Comprehensive Cancer Network: NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines). Cervical Cancer Version 1. 2013, https://www.nccn.org/professionals/physician_gls/f_guidelines.asp. Accession date: 2016-9-10.

3 

Monk BJ, Sill MW, McMeekin DS, Cohn DE, Ramondetta LM, Boardman CH, Benda J and Cella D: Phase III trial of four cisplatin-containing doublet combinations in stage IVB, recurrent, or persistent cervical carcinoma: A Gynecologic Oncology Group study. J Clin Oncol. 27:4649–4655. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Thigpen T: The role of chemotherapy in the management of carcinoma of the cervix. Cancer J. 9:425–432. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Kato R, Hasegawa K, Achiwa Y, Okamoto H, Torii Y, Oe S and Udagawa Y: Predicting nedaplatin sensitivity of cervical cancer using the histoculture drug response assay. Eur J Gynaecol Oncol. 32:381–386. 2011.PubMed/NCBI

6 

Yamamoto K, Kokawa K, Umesaki N, Nishimura R, Hasegawa K, Konishi I, Saji F, Nishida M, Noguchi H and Takizawa K: Phase I study of combination chemotherapy with irinotecan hydrochloride and nedaplatin for cervical squamous cell carcinoma: Japanese gynecologic oncology group study. Oncol Rep. 21:1005–1009. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Watanabe Y, Nakai H, Etoh T, Kanemura K, Tsuji I, Ishizu A and Hoshiai H: Feasibility study of docetaxel and nedaplatin for recurrent squamous cell carcinoma of the uterine cervix. Anticancer Res. 28(4C): 2385–2388. 2008.PubMed/NCBI

8 

Sultana H, Kigawa J, Kanamori Y, Itamochi H, Oishi T, Sato S, Kamazawa S, Ohwada M, Suzuki M and Terakawa N: Chemosensitivity and p53-Bax pathway-mediated apoptosis in patients with uterine cervical cancer. Ann Oncol. 14:214–219. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Zhao Y and Srivastava D: A developmental view of microRNA function. Trends Biochem Sci. 32:189–197. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Stefani G and Slack FJ: Small non-coding RNAs in animal development. Nat Rev Mol Cell Biol. 9:219–230. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Taipaleenmäki H, Bjerre Hokland L, Chen L, Kauppinen S and Kassem M: Mechanisms in endocrinology: Micro-RNAs: targets for enhancing osteoblast differentiation and bone formation. Eur J Endocrinol. 166:359–371. 2012. View Article : Google Scholar

13 

Vimalraj S and Selvamurugan N: MicroRNAs: Synthesis, gene regulation and osteoblast differentiation. Curr Issues Mol Biol. 15:7–18. 2013.

14 

Ni CW, Qiu H and Jo H: MicroRNA-663 upregulated by oscillatory shear stress plays a role in inflammatory response of endothelial cells. Am J Physiol Heart Circ Physiol. 300:H1762–H1769. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Guan YJ, Yang X, Wei L and Chen Q: miR-365: A mechanosensitive microRNA stimulates chondrocyte differentiation through targeting histone deacetylase 4. FASEB J. 25:4457–4466. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Liang D, Xu X, Deng F, Feng J, Zhang H, Liu Y, Zhang Y, Pan L, Liu Y, Zhang D, et al: miRNA-940 reduction contributes to human Tetralogy of Fallot development. J Cell Mol Med. 18:1830–1839. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Rajendiran S, Parwani AV, Hare RJ, Dasgupta S, Roby RK and Vishwanatha JK: MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1. Mol Cancer. 13:2502014. View Article : Google Scholar : PubMed/NCBI

18 

Song B, Zhang C, Li G, Jin G and Liu C: miR-940 inhibited pancreatic ductal adenocarcinoma growth by targeting MyD88. Cell Physiol Biochem. 35:1167–1177. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Yang HW, Liu GH, Liu YQ, Zhao HC, Yang Z, Zhao CL, Zhang XF and Ye H: Over-expression of microRNA-940 promotes cell proliferation by targeting GSK3β and sFRP1 in human pancreatic carcinoma. Biomed Pharmacother. 83:593–601. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Huang K, Tang Y, He L and Dai Y: MicroRNA-340 inhibits prostate cancer cell proliferation and metastasis by targeting the MDM2-p53 pathway. Oncol Rep. 35:887–895. 2016.PubMed/NCBI

21 

Lin SY, Chang CH, Wu HC, Lin CC, Chang KP, Yang CR, Huang CP, Hsu WH, Chang CT and Chen CJ: Proteome profiling of urinary exosomes identifies alpha 1-antitrypsin and H2B1K as diagnostic and prognostic biomarkers for urothelial carcinoma. Sci Rep. 6:344462016. View Article : Google Scholar : PubMed/NCBI

22 

Ma J, Sun F, Li C, Zhang Y, Xiao W, Li Z, Pan Q, Zeng H, Xiao G, Yao K, et al: Depletion of intermediate filament protein Nestin, a target of microRNA-940, suppresses tumorigenesis by inducing spontaneous DNA damage accumulation in human nasopharyngeal carcinoma. Cell Death Dis. 5:e13772014. View Article : Google Scholar : PubMed/NCBI

23 

Weber CEM, Luo C, Hotz-Wagenblatt A, Gardyan A, Kordass T, Holland-Letz T, Osen W and Eichmüller SB: miR-339-3p is a tumor suppressor in melanoma. Cancer Res. 76:3562–3571. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Hasegawa K, Kato R, Torii Y, Ichikawa R, Oe S and Udagawa Y: The relationship between ERCC1 expression and clinical outcome in patients with FIGO stage I to stage II uterine cervical adenocarcinoma. Int J Gynecol Cancer. 21:1479–1485. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Chung HH, Kim MK, Kim JW, Park NH, Song YS, Kang SB and Lee HP: XRCC1 R399Q polymorphism is associated with response to platinum-based neoadjuvant chemotherapy in bulky cervical cancer. Gynecol Oncol. 103:1031–1037. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Ozen M, Creighton CJ, Ozdemir M and Ittmann M: Widespread deregulation of microRNA expression in human prostate cancer. Oncogene. 27:1788–1793. 2008. View Article : Google Scholar

27 

Tamguney T and Stokoe D: New insights into PTEN. J Cell Sci. 120:4071–4079. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Martin J and Dufour JF: Tumor suppressor and hepatocellular carcinoma. World J Gastroenterol. 14:1720–1733. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Yao YJ, Ping XL, Zhang H, Chen FF, Lee PK, Ahsan H, Chen CJ, Lee PH, Peacocke M, Santella RM, et al: PTEN/MMAC1 mutations in hepatocellular carcinomas. Oncogene. 18:3181–3185. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Slipicevic A, Holm R, Nguyen MT, Bøhler PJ, Davidson B and Flørenes VA: Expression of activated Akt and PTEN in malignant melanomas: Relationship with clinical outcome. Am J Clin Pathol. 124:528–536. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Xu Z, Stokoe D, Kane LP and Weiss A: The inducible expression of the tumor suppressor gene PTEN promotes apoptosis and decreases cell size by inhibiting the PI3K/Akt pathway in Jurkat T cells. Cell Growth Differ. 13:285–296. 2002.PubMed/NCBI

32 

Weng LP, Brown JL and Eng C: PTEN coordinates G(1) arrest by down-regulating cyclin D1 via its protein phosphatase activity and up-regulating p27 via its lipid phosphatase activity in a breast cancer model. Hum Mol Genet. 10:599–604. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Chu IM, Hengst L and Slingerland JM: The Cdk inhibitor p27 in human cancer: Prognostic potential and relevance to anticancer therapy. Nat Rev Cancer. 8:253–267. 2008. View Article : Google Scholar : PubMed/NCBI

34 

le Sage C, Nagel R and Agami R: Diverse ways to control p27Kip1 function: miRNAs come into play. Cell Cycle. 6:2742–2749. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Chu I, Sun J, Arnaout A, Kahn H, Hanna W, Narod S, Sun P, Tan CK, Hengst L and Slingerland J: p27 phosphorylation by Src regulates inhibition of cyclin E-Cdk2. Cell. 128:281–294. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Vermeulen K, Van Bockstaele DR and Berneman ZN: The cell cycle: A review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif. 36:131–149. 2003. View Article : Google Scholar : PubMed/NCBI

37 

Pateras IS, Apostolopoulou K, Koutsami M, Evangelou K, Tsantoulis P, Liloglou T, Nikolaidis G, Sigala F, Kittas C, Field JK, et al: Downregulation of the KIP family members p27(KIP1) and p57(KIP2) by SKP2 and the role of methylation in p57(KIP2) inactivation in nonsmall cell lung cancer. Int J Cancer. 119:2546–2556. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Su K, Wang C, Zhang Y, Cai Y, Zhang Y and Zhao Q: miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition. Int J Oncol 50: 1211-1220, 2017.
APA
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., & Zhao, Q. (2017). miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition. International Journal of Oncology, 50, 1211-1220. https://doi.org/10.3892/ijo.2017.3897
MLA
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., Zhao, Q."miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition". International Journal of Oncology 50.4 (2017): 1211-1220.
Chicago
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., Zhao, Q."miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition". International Journal of Oncology 50, no. 4 (2017): 1211-1220. https://doi.org/10.3892/ijo.2017.3897
Copy and paste a formatted citation
x
Spandidos Publications style
Su K, Wang C, Zhang Y, Cai Y, Zhang Y and Zhao Q: miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition. Int J Oncol 50: 1211-1220, 2017.
APA
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., & Zhao, Q. (2017). miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition. International Journal of Oncology, 50, 1211-1220. https://doi.org/10.3892/ijo.2017.3897
MLA
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., Zhao, Q."miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition". International Journal of Oncology 50.4 (2017): 1211-1220.
Chicago
Su, K., Wang, C., Zhang, Y., Cai, Y., Zhang, Y., Zhao, Q."miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition". International Journal of Oncology 50, no. 4 (2017): 1211-1220. https://doi.org/10.3892/ijo.2017.3897
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