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Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer

  • Authors:
    • Mengnan Zhang
    • Feng Ma
    • Ruyi Xie
    • Yao Wu
    • Meiyan Wu
    • Pei Zhang
    • Ying Peng
    • Jinjun Zhao
    • Jing Xiong
    • Aimin Li
    • Cheng Kequan
    • Yali Zhang
    • Side Liu
    • Jide Wang
    • Xueqing Chen
  • View Affiliations / Copyright

    Affiliations: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Rheumatism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1555-1566
    |
    Published online on: April 5, 2017
       https://doi.org/10.3892/ijo.2017.3952
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Abstract

The adaptor protein Srcin1 is a novel Src-binding protein that regulates Src activation through C-terminal Src kinase (Csk). Srcin1 behaves as a tumour suppressor in breast cancer, but the role of Srcin1 in the development of colorectal cancer (CRC) remains unknown. In the present study, Srcin1 expression in normal tissue was examined by tissue microarray and assessed by immunohistochemistry in 10 patients. In addition, the biological impact of Srcin1 knockdown on CRC cells was investigated in vitro and in vivo. The results showed that Srcin1 was expressed in different types of normal human tissues, whereas its expression was increased in human CRC tissues. Srcin1 expression also correlated with tumour progression. The suppression of Srcin1 induced cell differentiation and G0/G1 cell cycle arrest. Furthermore, Srcin1 increased cell growth as well as the capacity of migration and invasion in CRC cells. Srcin1 induced the activation of the Wnt/β-catenin signalling pathway. Moreover, Srcin1 suppression sensitized cancer cells to 5-fluorouracil (5-FU)-induced apoptosis in vitro and in vivo. Together, these results demonstrate that Srcin1 contributes to CRC carcinogenesis, invasion and metastasis. These findings provide a rationale for a mechanistic approach to CRC treatment based on the development of Srcin1-targeted therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Ma F, Xie R, Wu Y, Wu M, Zhang P, Peng Y, Zhao J, Xiong J, Li A, Li A, et al: Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. Int J Oncol 50: 1555-1566, 2017.
APA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P. ... Chen, X. (2017). Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. International Journal of Oncology, 50, 1555-1566. https://doi.org/10.3892/ijo.2017.3952
MLA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50.5 (2017): 1555-1566.
Chicago
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50, no. 5 (2017): 1555-1566. https://doi.org/10.3892/ijo.2017.3952
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Ma F, Xie R, Wu Y, Wu M, Zhang P, Peng Y, Zhao J, Xiong J, Li A, Li A, et al: Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. Int J Oncol 50: 1555-1566, 2017.
APA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P. ... Chen, X. (2017). Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. International Journal of Oncology, 50, 1555-1566. https://doi.org/10.3892/ijo.2017.3952
MLA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50.5 (2017): 1555-1566.
Chicago
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50, no. 5 (2017): 1555-1566. https://doi.org/10.3892/ijo.2017.3952
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