Open Access

Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer

  • Authors:
    • Mengnan Zhang
    • Feng Ma
    • Ruyi Xie
    • Yao Wu
    • Meiyan Wu
    • Pei Zhang
    • Ying Peng
    • Jinjun Zhao
    • Jing Xiong
    • Aimin Li
    • Cheng Kequan
    • Yali Zhang
    • Side Liu
    • Jide Wang
    • Xueqing Chen
  • View Affiliations

  • Published online on: April 5, 2017     https://doi.org/10.3892/ijo.2017.3952
  • Pages: 1555-1566
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The adaptor protein Srcin1 is a novel Src-binding protein that regulates Src activation through C-terminal Src kinase (Csk). Srcin1 behaves as a tumour suppressor in breast cancer, but the role of Srcin1 in the development of colorectal cancer (CRC) remains unknown. In the present study, Srcin1 expression in normal tissue was examined by tissue microarray and assessed by immunohistochemistry in 10 patients. In addition, the biological impact of Srcin1 knockdown on CRC cells was investigated in vitro and in vivo. The results showed that Srcin1 was expressed in different types of normal human tissues, whereas its expression was increased in human CRC tissues. Srcin1 expression also correlated with tumour progression. The suppression of Srcin1 induced cell differentiation and G0/G1 cell cycle arrest. Furthermore, Srcin1 increased cell growth as well as the capacity of migration and invasion in CRC cells. Srcin1 induced the activation of the Wnt/β-catenin signalling pathway. Moreover, Srcin1 suppression sensitized cancer cells to 5-fluorouracil (5-FU)-induced apoptosis in vitro and in vivo. Together, these results demonstrate that Srcin1 contributes to CRC carcinogenesis, invasion and metastasis. These findings provide a rationale for a mechanistic approach to CRC treatment based on the development of Srcin1-targeted therapies.
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May-2017
Volume 50 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhang M, Ma F, Xie R, Wu Y, Wu M, Zhang P, Peng Y, Zhao J, Xiong J, Li A, Li A, et al: Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. Int J Oncol 50: 1555-1566, 2017
APA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P. ... Chen, X. (2017). Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. International Journal of Oncology, 50, 1555-1566. https://doi.org/10.3892/ijo.2017.3952
MLA
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50.5 (2017): 1555-1566.
Chicago
Zhang, M., Ma, F., Xie, R., Wu, Y., Wu, M., Zhang, P., Peng, Y., Zhao, J., Xiong, J., Li, A., Kequan, C., Zhang, Y., Liu, S., Wang, J., Chen, X."Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer". International Journal of Oncology 50, no. 5 (2017): 1555-1566. https://doi.org/10.3892/ijo.2017.3952