Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
July-2017 Volume 51 Issue 1

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 51 Issue 1

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer

  • Authors:
    • Birdal Bilir
    • Nitya V. Sharma
    • Jeongseok Lee
    • Bato Hammarstrom
    • Aud Svindland
    • Omer Kucuk
    • Carlos S. Moreno
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA, Department of Urology, Institute of Cancer Research, Oslo University Hospital, Oslo, Norway, Department of Clinical Medicine, University of Oslo, Oslo, Norway, Department of Hematology and Medical Oncology, Emory University, Atlanta, GA, USA
  • Pages: 223-234
    |
    Published online on: May 24, 2017
       https://doi.org/10.3892/ijo.2017.4017
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Epidemiological studies have shown that dietary compounds have significant effects on prostate carcinogenesis. Among dietary agents, genistein, the major isoflavone in soybean, is of particular interest because high consumption of soy products has been associated with a low incidence of prostate cancer, suggesting a preventive role of genistein in prostate cancer. In spite of numerous studies to understand the effects of genistein on prostate cancer, the mechanisms of action have not been fully elucidated. We investigated the differences in methylation and gene expression levels of prostate specimens from a clinical trial of genistein supplementation prior to prostatectomy using Illumina HumanMethylation450 and Illumina HumanHT-12 v4 Expression BeadChip Microarrays. The present study was a randomized, placebo-controlled, double-blind clinical trial on Norwegian patients who received 30 mg genistein or placebo capsules daily for 3-6 weeks before prostatectomy. Gene expression changes were validated by quantitative PCR (qPCR). Whole genome methylation and expression profiling identified differentially methylated sites and expressed genes between placebo and genistein groups. Differentially regulated genes were involved in developmental processes, stem cell markers, proliferation and transcriptional regulation. Enrichment analysis suggested overall reduction in MYC activity and increased PTEN activity in genistein-treated patients. These findings highlight the effects of genistein on global changes in gene expression in prostate cancer and its effects on molecular pathways involved in prostate tumorigenesis.
View Figures

Figure 1

Figure 2

View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Prostate Cancer Facts and Figures 2016. American Cancer Society Inc; Atlanta, GA: 2016

3 

Dunn MW and Kazer MW: Prostate cancer overview. Semin Oncol Nurs. 27:241–250. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Sartor AO: Progression of metastatic castrate-resistant prostate cancer: Impact of therapeutic intervention in the post-docetaxel space. J Hematol Oncol. 4:182011. View Article : Google Scholar : PubMed/NCBI

5 

Skolarus TA, Wolf AMD, Erb NL, Brooks DD, Rivers BM, Underwood W III, Salner AL, Zelefsky MJ, Aragon-Ching JB, Slovin SF, et al: American Cancer Society prostate cancer survivorship care guidelines. CA Cancer J Clin. 64:225–249. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Hieronymus H, Schultz N, Gopalan A, Carver BS, Chang MT, Xiao Y, Heguy A, Huberman K, Bernstein M, Assel M, et al: Copy number alteration burden predicts prostate cancer relapse. Proc Natl Acad Sci USA. 111:11139–11144. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Ferlay J, Bray F, Pisani P and Parkin DM: Globocan 2000: Cancer Incidence, Mortality and Prevalence Worldwide. IARC Press; Lyon: 2001

8 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Kimura T: East meets West: Ethnic differences in prostate cancer epidemiology between East Asians and Caucasians. Chin J Cancer. 31:421–429. 2012. View Article : Google Scholar

10 

Reinli K and Block G: Phytoestrogen content of foods–a compendium of literature values. Nutr Cancer. 26:123–148. 1996. View Article : Google Scholar

11 

Adlercreutz H, Markkanen H and Watanabe S: Plasma concentrations of phyto-oestrogens in Japanese men. Lancet. 342:1209–1210. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Morton MS, Chan PS, Cheng C, Blacklock N, Matos-Ferreira A, Abranches-Monteiro L, Correia R, Lloyd S and Griffiths K: Lignans and isoflavonoids in plasma and prostatic fluid in men: Samples from Portugal, Hong Kong, and the United Kingdom. Prostate. 32:122–128. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Hwang YW, Kim SY, Jee SH, Kim YN and Nam CM: Soy food consumption and risk of prostate cancer: A meta-analysis of observational studies. Nutr Cancer. 61:598–606. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Van Poppel H and Tombal B: Chemoprevention of prostate cancer with nutrients and supplements. Cancer Manag Res. 3:91–100. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Barnes S, Peterson TG and Coward L: Rationale for the use of genistein-containing soy matrices in chemoprevention trials for breast and prostate cancer. J Cell Biochem (Suppl). S22:181–187. 1995. View Article : Google Scholar

16 

Takimoto CH, Glover K, Huang X, Hayes SA, Gallot L, Quinn M, Jovanovic BD, Shapiro A, Hernandez L, Goetz A, et al: Phase I pharmacokinetic and pharmacodynamic analysis of unconjugated soy isoflavones administered to individuals with cancer. Cancer Epidemiol Biomarkers Prev. 12:1213–1221. 2003.PubMed/NCBI

17 

Gardner CD, Oelrich B, Liu JP, Feldman D, Franke AA and Brooks JD: Prostatic soy isoflavone concentrations exceed serum levels after dietary supplementation. Prostate. 69:719–726. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Bloedon LT, Jeffcoat AR, Lopaczynski W, Schell MJ, Black TM, Dix KJ, Thomas BF, Albright C, Busby MG, Crowell JA, et al: Safety and pharmacokinetics of purified soy isoflavones: Single-dose administration to postmenopausal women. Am J Clin Nutr. 76:1126–1137. 2002.PubMed/NCBI

19 

Yildiz F: Phytoestrogens in functional foods. CRC Press; Boca Raton, FL: 2005, https://doi.org/10.1201/9781420027594. View Article : Google Scholar

20 

Morito K, Hirose T, Kinjo J, Hirakawa T, Okawa M, Nohara T, Ogawa S, Inoue S, Muramatsu M and Masamune Y: Interaction of phytoestrogens with estrogen receptors alpha and beta. Biol Pharm Bull. 24:351–356. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Chang WY and Prins GS: Estrogen receptor-beta: Implications for the prostate gland. Prostate. 40:115–124. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Kumar R, Verma V, Jain A, Jain RK, Maikhuri JP and Gupta G: Synergistic chemoprotective mechanisms of dietary phytoestrogens in a select combination against prostate cancer. J Nutr Biochem. 22:723–731. 2011. View Article : Google Scholar

23 

Wang J, Eltoum IE and Lamartiniere CA: Genistein chemoprevention of prostate cancer in TRAMP mice. J Carcinog. 6:32007. View Article : Google Scholar : PubMed/NCBI

24 

Davis JN, Kucuk O and Sarkar FH: Expression of prostate-specific antigen is transcriptionally regulated by genistein in prostate cancer cells. Mol Carcinog. 34:91–101. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Bektic J, Berger AP, Pfeil K, Dobler G, Bartsch G and Klocker H: Androgen receptor regulation by physiological concentrations of the isoflavonoid genistein in androgen-dependent LNCaP cells is mediated by estrogen receptor beta. Eur Urol. 45:245–251; discussion 251. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Shen JC, Klein RD, Wei Q, Guan Y, Contois JH, Wang TT, Chang S and Hursting SD: Low-dose genistein induces cyclin-dependent kinase inhibitors and G(1) cell-cycle arrest in human prostate cancer cells. Mol Carcinog. 29:92–102. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Davis JN, Singh B, Bhuiyan M and Sarkar FH: Genistein-induced upregulation of p21WAF1, downregulation of cyclin B, and induction of apoptosis in prostate cancer cells. Nutr Cancer. 32:123–131. 1998. View Article : Google Scholar

28 

Raffoul JJ, Wang Y, Kucuk O, Forman JD, Sarkar FH and Hillman GG: Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest. BMC Cancer. 6:1072006. View Article : Google Scholar : PubMed/NCBI

29 

Li Y and Sarkar FH: Down-regulation of invasion and angiogenesis-related genes identified by cDNA microarray analysis of PC3 prostate cancer cells treated with genistein. Cancer Lett. 186:157–164. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Guo Y, Wang S, Hoot DR and Clinton SK: Suppression of VEGF-mediated autocrine and paracrine interactions between prostate cancer cells and vascular endothelial cells by soy isoflavones. J Nutr Biochem. 18:408–417. 2007. View Article : Google Scholar

31 

Li Y, Che M, Bhagat S, Ellis KL, Kucuk O, Doerge DR, Abrams J, Cher ML and Sarkar FH: Regulation of gene expression and inhibition of experimental prostate cancer bone metastasis by dietary genistein. Neoplasia. 6:354–363. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Zhang LL, Li L, Wu DP, Fan JH, Li X, Wu KJ, Wang XY and He DL: A novel anti-cancer effect of genistein: Reversal of epithelial mesenchymal transition in prostate cancer cells. Acta Pharmacol Sin. 29:1060–1068. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Kumi-Diaka JK, Hassanhi M, Merchant K and Horman V: Influence of genistein isoflavone on matrix metalloproteinase-2 expression in prostate cancer cells. J Med Food. 9:491–497. 2006. View Article : Google Scholar

34 

Kazi A, Daniel KG, Smith DM, Kumar NB and Dou QP: Inhibition of the proteasome activity, a novel mechanism associated with the tumor cell apoptosis-inducing ability of genistein. Biochem Pharmacol. 66:965–976. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Kumi-Diaka J, Sanderson NA and Hall A: The mediating role of caspase-3 protease in the intracellular mechanism of genistein-induced apoptosis in human prostatic carcinoma cell lines, DU145 and LNCaP. Biol Cell. 92:595–604. 2000. View Article : Google Scholar

36 

Lee J, Ju J, Park S, Hong SJ and Yoon S: Inhibition of IGF-1 signaling by genistein: Modulation of E-cadherin expression and downregulation of β-catenin signaling in hormone refractory PC-3 prostate cancer cells. Nutr Cancer. 64:153–162. 2012. View Article : Google Scholar

37 

Xu L and Bergan RC: Genistein inhibits matrix metalloproteinase type 2 activation and prostate cancer cell invasion by blocking the transforming growth factor beta-mediated activation of mitogen-activated protein kinase-activated protein kinase 2-27-kDa heat shock protein pathway. Mol Pharmacol. 70:869–877. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Davis JN, Kucuk O and Sarkar FH: Genistein inhibits NF-kappaB activation in prostate cancer cells. Nutr Cancer. 35:167–174. 1999. View Article : Google Scholar

39 

Li Y and Sarkar FH: Inhibition of nuclear factor kappaB activation in PC3 cells by genistein is mediated via Akt signaling pathway. Clin Cancer Res. 8:2369–2377. 2002.PubMed/NCBI

40 

Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M and Fukami Y: Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 262:5592–5595. 1987.PubMed/NCBI

41 

Park CE, Yun H, Lee EB, Min BI, Bae H, Choe W, Kang I, Kim SS and Ha J: The antioxidant effects of genistein are associated with AMP-activated protein kinase activation and PTEN induction in prostate cancer cells. J Med Food. 13:815–820. 2010. View Article : Google Scholar : PubMed/NCBI

42 

Raschke M, Rowland IR, Magee PJ and Pool-Zobel BL: Genistein protects prostate cells against hydrogen peroxide-induced DNA damage and induces expression of genes involved in the defence against oxidative stress. Carcinogenesis. 27:2322–2330. 2006. View Article : Google Scholar : PubMed/NCBI

43 

Swami S, Krishnan AV, Moreno J, Bhattacharyya RS, Gardner C, Brooks JD, Peehl DM and Feldman D: Inhibition of prostaglandin synthesis and actions by genistein in human prostate cancer cells and by soy isoflavones in prostate cancer patients. Int J Cancer. 124:2050–2059. 2009. View Article : Google Scholar : PubMed/NCBI

44 

Adjakly M, Bosviel R, Rabiau N, Boiteux JP, Bignon YJ, Guy L and Bernard-Gallon D: DNA methylation and soy phytoestrogens: Quantitative study in DU-145 and PC-3 human prostate cancer cell lines. Epigenomics. 3:795–803. 2011. View Article : Google Scholar : PubMed/NCBI

45 

Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, Boiteux JP, Fontana L, Bignon YJ, Guy L, et al: Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells. In Vivo. 24:393–400. 2010.PubMed/NCBI

46 

Fang MZ, Chen D, Sun Y, Jin Z, Christman JK and Yang C: Reversal of hypermethylation and reactivation of 16INK4a, RARbeta, and MGMT genes by genistein and other isoflavones from soy. Clin Cancer Res. 11:7033–7041. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Majid S, Dar AA, Shahryari V, Hirata H, Ahmad A, Saini S, Tanaka Y, Dahiya AV and Dahiya R: Genistein reverses hyper-methylation and induces active histone modifications in tumor suppressor gene B-Cell translocation gene 3 in prostate cancer. Cancer. 116:66–76. 2010.

48 

Kikuno N, Shiina H, Urakami S, Kawamoto K, Hirata H, Tanaka Y, Majid S, Igawa M and Dahiya R: Genistein mediated histone acetylation and demethylation activates tumor suppressor genes in prostate cancer cells. Int J Cancer. 123:552–560. 2008. View Article : Google Scholar : PubMed/NCBI

49 

Li Y, Kong D, Ahmad A, Bao B, Dyson G and Sarkar FH: Epigenetic deregulation of miR-29a and miR-1256 by isoflavone contributes to the inhibition of prostate cancer cell growth and invasion. Epigenetics. 7:940–949. 2012. View Article : Google Scholar : PubMed/NCBI

50 

Rabiau N, Trraf HK, Adjakly M, Bosviel R, Guy L, Fontana L, Bignon YJ and Bernard-Gallon DJ: miRNAs differentially expressed in prostate cancer cell lines after soy treatment. In Vivo. 25:917–921. 2011.PubMed/NCBI

51 

Chen Y, Zaman MS, Deng G, Majid S, Saini S, Liu J, Tanaka Y and Dahiya R: MicroRNAs 221/222 and genistein-mediated regulation of ARHI tumor suppressor gene in prostate cancer. Cancer Prev Res (Phila). 4:76–86. 2011. View Article : Google Scholar

52 

Chiyomaru T, Yamamura S, Fukuhara S, Hidaka H, Majid S, Saini S, Arora S, Deng G, Shahryari V, Chang I, et al: Genistein up-regulates tumor suppressor microRNA-574-3p in prostate cancer. PLoS One. 8:e589292013. View Article : Google Scholar : PubMed/NCBI

53 

Hillman GG, Forman JD, Kucuk O, Yudelev M, Maughan RL, Rubio J, Layer A, Tekyi-Mensah S, Abrams J and Sarkar FH: Genistein potentiates the radiation effect on prostate carcinoma cells. Clin Cancer Res. 7:382–390. 2001.PubMed/NCBI

54 

Li Y, Kucuk O, Hussain M, Abrams J, Cher ML and Sarkar FH: Antitumor and antimetastatic activities of docetaxel are enhanced by genistein through regulation of osteoprotegerin/receptor activator of nuclear factor-kappaB (RANK)/RANK ligand/MMP-9 signaling in prostate cancer. Cancer Res. 66:4816–4825. 2006. View Article : Google Scholar : PubMed/NCBI

55 

Spagnuolo C, Russo GL, Orhan IE, Habtemariam S, Daglia M, Sureda A, Nabavi SF, Devi KP, Loizzo MR, Tundis R, et al: Genistein and cancer: Current status, challenges, and future directions. Adv Nutr. 6:408–419. 2015. View Article : Google Scholar : PubMed/NCBI

56 

Katoh M: Network of WNT and other regulatory signaling cascades in pluripotent stem cells and cancer stem cells. Curr Pharm Biotechnol. 12:160–170. 2011. View Article : Google Scholar

57 

Mori R, Wang Q, Danenberg KD, Pinski JK and Danenberg PV: Both beta-actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer. Prostate. 68:1555–1560. 2008. View Article : Google Scholar : PubMed/NCBI

58 

Reich M, Liefeld T, Gould J, Lerner J, Tamayo P and Mesirov JP: GenePattern 2.0. Nat Genet. 38:500–501. 2006. View Article : Google Scholar : PubMed/NCBI

59 

Eisen MB, Spellman PT, Brown PO and Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 95:14863–14868. 1998. View Article : Google Scholar : PubMed/NCBI

60 

Saldanha AJ: Java Treeview - extensible visualization of micro-array data. Bioinformatics. 20:3246–3248. 2004. View Article : Google Scholar : PubMed/NCBI

61 

Barfield RT, Kilaru V, Smith AK and Conneely KN: CpGassoc: An R function for analysis of DNA methylation microarray data. Bioinformatics. 28:1280–1281. 2012. View Article : Google Scholar : PubMed/NCBI

62 

http://www.qiagen.com/ingenuity. Qiagen's Ingenuity Pathway Analysis.

63 

Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC and Lempicki RA: DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 4:32003. View Article : Google Scholar

64 

Wang Z and Chen H: Genistein increases gene expression by demethylation of WNT5a promoter in colon cancer cell line SW1116. Anticancer Res. 30:4537–4545. 2010.PubMed/NCBI

65 

Gao BN and Gilman AG: Cloning and expression of a widely distributed (type IV) adenylyl cyclase. Proc Natl Acad Sci USA. 88:10178–10182. 1991. View Article : Google Scholar : PubMed/NCBI

66 

Brikun I, Nusskern D, Gillen D, Lynn A, Murtagh D, Feczko J, Nelson WG and Freije D: A panel of DNA methylation markers reveals extensive methylation in histologically benign prostate biopsy cores from cancer patients. Biomark Res. 2:252014. View Article : Google Scholar : PubMed/NCBI

67 

Monti E, Bonten E, D'Azzo A, Bresciani R, Venerando B, Borsani G, Schauer R and Tettamanti G: Sialidases in vertebrates: A family of enzymes tailored for several cell functions. Adv Carbohydr Chem Biochem. 64:403–479. 2010. View Article : Google Scholar : PubMed/NCBI

68 

Uemura T, Shiozaki K, Yamaguchi K, Miyazaki S, Satomi S, Kato K, Sakuraba H and Miyagi T: Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin beta4. Oncogene. 28:1218–1229. 2009. View Article : Google Scholar : PubMed/NCBI

69 

Kato T, Wang Y, Yamaguchi K, Milner CM, Shineha R, Satomi S and Miyagi T: Overexpressing of lysosomal-type sialidase leads to suppression of metastasis associated with reversion of malignant phenotype in murine B16 melanoma cells. Int J Cancer. 92:797–804. 2001. View Article : Google Scholar : PubMed/NCBI

70 

Chang S, He S, Qiu G, Lu J, Wang J, Liu J, Fan L, Zhao W and Che X: MicroRNA-125b promotes invasion and metastasis of gastric cancer by targeting STARD13 and NEU1. Tumour Biol. 37:12141–12151. 2016. View Article : Google Scholar : PubMed/NCBI

71 

Haxho F, Neufeld RJ and Szewczuk MR: Neuraminidase-1: A novel therapeutic target in multistage tumorigenesis. Oncotarget. 7:40860–40881. 2016.PubMed/NCBI

72 

Liu L, Chen X, Wang Y, Qu Z, Lu Q, Zhao J, Yan X, Zhang H and Zhou Y: Notch3 is important for TGF-β-induced epithelial-mesenchymal transition in non-small cell lung cancer bone metastasis by regulating ZEB-1. Cancer Gene Ther. 21:364–372. 2014. View Article : Google Scholar : PubMed/NCBI

73 

Danza G, Di Serio C, Ambrosio MR, Sturli N, Lonetto G, Rosati F, Rocca BJ, Ventimiglia G, del Vecchio MT, Prudovsky I, et al: Notch3 is activated by chronic hypoxia and contributes to the progression of human prostate cancer. Int J Cancer. 133:2577–2586. 2013.PubMed/NCBI

74 

Terada N, Shiraishi T, Zeng Y, Aw-Yong KM, Mooney SM, Liu Z, Takahashi S, Luo J, Lupold SE, Kulkarni P, et al: Correlation of Sprouty1 and Jagged1 with aggressive prostate cancer cells with different sensitivities to androgen deprivation. J Cell Biochem. 115:1505–1515. 2014. View Article : Google Scholar : PubMed/NCBI

75 

Pedrosa AR, Graça JL, Carvalho S, Peleteiro MC, Duarte A and Trindade A: Notch signaling dynamics in the adult healthy prostate and in prostatic tumor development. Prostate. 76:80–96. 2016. View Article : Google Scholar

76 

Pedrosa AR, Trindade A, Carvalho C, Graça J, Carvalho S, Peleteiro MC, Adams RH and Duarte A: Endothelial Jagged1 promotes solid tumor growth through both pro-angiogenic and angiocrine functions. Oncotarget. 6:24404–24423. 2015. View Article : Google Scholar : PubMed/NCBI

77 

Danza G, Di Serio C, Rosati F, Lonetto G, Sturli N, Kacer D, Pennella A, Ventimiglia G, Barucci R, Piscazzi A, et al: Notch signaling modulates hypoxia-induced neuroendocrine differentiation of human prostate cancer cells. Mol Cancer Res. 10:230–238. 2012. View Article : Google Scholar

78 

Rampias T, Vgenopoulou P, Avgeris M, Polyzos A, Stravodimos K, Valavanis C, Scorilas A and Klinakis A: A new tumor suppressor role for the Notch pathway in bladder cancer. Nat Med. 20:1199–1205. 2014. View Article : Google Scholar : PubMed/NCBI

79 

Hernandez Tejada FN, Galvez Silva JR and Zweidler-McKay PA: The challenge of targeting notch in hematologic malignancies. Front Pediatr. 2:542014. View Article : Google Scholar : PubMed/NCBI

80 

Giachino C, Boulay JL, Ivanek R, Alvarado A, Tostado C, Lugert S, Tchorz J, Coban M, Mariani L, Bettler B, et al: A tumor suppressor function for Notch signaling in forebrain tumor subtypes. Cancer Cell. 28:730–742. 2015. View Article : Google Scholar : PubMed/NCBI

81 

Jaskula-Sztul R, Eide J, Tesfazghi S, Dammalapati A, Harrison AD, Yu XM, Scheinebeck C, Winston-McPherson G, Kupcho KR, Robers MB, et al: Tumor-suppressor role of Notch3 in medullary thyroid carcinoma revealed by genetic and pharmacological induction. Mol Cancer Ther. 14:499–512. 2015. View Article : Google Scholar

82 

Sriuranpong V, Borges MW, Ravi RK, Arnold DR, Nelkin BD, Baylin SB and Ball DW: Notch signaling induces cell cycle arrest in small cell lung cancer cells. Cancer Res. 61:3200–3205. 2001.PubMed/NCBI

83 

Liu YL, Zhang GQ, Yang Y, Zhang CY, Fu RX and Yang YM: Genistein induces G2/M arrest in gastric cancer cells by increasing the tumor suppressor PTEN expression. Nutr Cancer. 65:1034–1041. 2013. View Article : Google Scholar : PubMed/NCBI

84 

Su SJ, Yeh TM, Chuang WJ, Ho CL, Chang KL, Cheng HL, Liu HS, Cheng HL, Hsu PY and Chow NH: The novel targets for anti-angiogenesis of genistein on human cancer cells. Biochem Pharmacol. 69:307–318. 2005. View Article : Google Scholar : PubMed/NCBI

85 

Jagadeesh S, Kyo S and Banerjee PP: Genistein represses telomerase activity via both transcriptional and posttranslational mechanisms in human prostate cancer cells. Cancer Res. 66:2107–2115. 2006. View Article : Google Scholar : PubMed/NCBI

86 

Mahmoud AM, Al-Alem U, Ali MM and Bosland MC: Genistein increases estrogen receptor beta expression in prostate cancer via reducing its promoter methylation. J Steroid Biochem Mol Biol. 152:62–75. 2015. View Article : Google Scholar : PubMed/NCBI

87 

Whirledge S, Senbanjo LT and Cidlowski JA: Genistein disrupts glucocorticoid receptor signaling in human uterine endometrial Ishikawa cells. Environ Health Perspect. 123:80–87. 2015.

88 

Bhamre S, Sahoo D, Tibshirani R, Dill DL and Brooks JD: Gene expression changes induced by genistein in the prostate cancer cell line LNCaP. Open Prostate Cancer J. 3:86–98. 2010. View Article : Google Scholar

89 

Ghaemi A, Soleimanjahi H, Razeghi S, Gorji A, Tabaraei A, Moradi A, Alizadeh A and Vakili MA: Genistein induces a protective immunomodulatory effect in a mouse model of cervical cancer. Iran J Immunol. 9:119–127. 2012.PubMed/NCBI

90 

Korde LA, Wu AH, Fears T, Nomura AM, West DW, Kolonel LN, Pike MC, Hoover RN and Ziegler RG: Childhood soy intake and breast cancer risk in Asian American women. Cancer Epidemiol Biomarkers Prev. 18:1050–1059. 2009. View Article : Google Scholar : PubMed/NCBI

91 

Lee SA, Shu XO, Li H, Yang G, Cai H, Wen W, Ji BT, Gao J, Gao YT and Zheng W: Adolescent and adult soy food intake and breast cancer risk: Results from the Shanghai Women's Health Study. Am J Clin Nutr. 89:1920–1926. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Bilir B, Sharma NV, Lee J, Hammarstrom B, Svindland A, Kucuk O and Moreno CS: Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer. Int J Oncol 51: 223-234, 2017.
APA
Bilir, B., Sharma, N.V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., & Moreno, C.S. (2017). Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer. International Journal of Oncology, 51, 223-234. https://doi.org/10.3892/ijo.2017.4017
MLA
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51.1 (2017): 223-234.
Chicago
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51, no. 1 (2017): 223-234. https://doi.org/10.3892/ijo.2017.4017
Copy and paste a formatted citation
x
Spandidos Publications style
Bilir B, Sharma NV, Lee J, Hammarstrom B, Svindland A, Kucuk O and Moreno CS: Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer. Int J Oncol 51: 223-234, 2017.
APA
Bilir, B., Sharma, N.V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., & Moreno, C.S. (2017). Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer. International Journal of Oncology, 51, 223-234. https://doi.org/10.3892/ijo.2017.4017
MLA
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51.1 (2017): 223-234.
Chicago
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51, no. 1 (2017): 223-234. https://doi.org/10.3892/ijo.2017.4017
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team