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Article

In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging

  • Authors:
    • Annasofia Anemone
    • Lorena Consolino
    • Laura Conti
    • Francesca Reineri
    • Federica Cavallo
    • Silvio Aime
    • Dario Livio Longo
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biotechnology and Health Sciences, University of Turin, 10126 Turin, Italy, National Research Council of Italy (CNR), Institute of Biostructure and Bioimaging, Molecular Imaging Center, 10126 Turin, Italy
  • Pages: 498-506
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/ijo.2017.4029
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Abstract

Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo extracellular tumour pH. We examined whether MRI-CEST pH mapping can monitor in vivo changes in tumour acidosis for assessing treatment response to DCA. Cell viability and extracellular pH were assessed in TS/A breast cancer cells treated with 1-10 mM DCA for 24 h in normoxia or hypoxia (1% O2) conditions. Extracellular tumour pH values were measured in vivo by MRI-CEST pH mapping of TS/A tumour-bearing mice before, three days and fifteen days after DCA or saline treatment. Reduced extracellular acidification and vitality were observed in DCA-treated TS/A cells. Tumour-bearing mice showed a marked and significant increase of tumour extracellular pH at 3 days post-DCA treatment, reflecting DCA-induced glycolysis inhibition, as confirmed by reduced lactate production. After 15 days of DCA treatment, the onset of resistance to DCA was observed, with recover of tumour extracellular acidification and lactate levels that returned to baseline values. A significant correlation was observed between tumour extracellular pH values and lactate levels (r= -0.97, P<0.05). These results suggest that MRI-CEST pH imaging is a promising tool to monitor the early response and efficacy of cancer metabolic targeting drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Anemone A, Consolino L, Conti L, Reineri F, Cavallo F, Aime S and Longo DL: In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging. Int J Oncol 51: 498-506, 2017.
APA
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., & Longo, D.L. (2017). In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging. International Journal of Oncology, 51, 498-506. https://doi.org/10.3892/ijo.2017.4029
MLA
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., Longo, D. L."In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging". International Journal of Oncology 51.2 (2017): 498-506.
Chicago
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., Longo, D. L."In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging". International Journal of Oncology 51, no. 2 (2017): 498-506. https://doi.org/10.3892/ijo.2017.4029
Copy and paste a formatted citation
x
Spandidos Publications style
Anemone A, Consolino L, Conti L, Reineri F, Cavallo F, Aime S and Longo DL: In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging. Int J Oncol 51: 498-506, 2017.
APA
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., & Longo, D.L. (2017). In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging. International Journal of Oncology, 51, 498-506. https://doi.org/10.3892/ijo.2017.4029
MLA
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., Longo, D. L."In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging". International Journal of Oncology 51.2 (2017): 498-506.
Chicago
Anemone, A., Consolino, L., Conti, L., Reineri, F., Cavallo, F., Aime, S., Longo, D. L."In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging". International Journal of Oncology 51, no. 2 (2017): 498-506. https://doi.org/10.3892/ijo.2017.4029
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