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Article

Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer

  • Authors:
    • Takahisa Yamashita
    • Morihiro Higashi
    • Shuji Momose
    • Makoto Morozumi
    • Jun-Ichi Tamaru
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe-shi, Saitama 350-8550, Japan, Department of Urology, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe-shi, Saitama 350-8550, Japan
  • Pages: 579-586
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/ijo.2017.4031
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Abstract

The development and acquisition of multiple drug resistance in cancer cells remain a major obstacle in the treatment of bladder cancer. Nuclear translocation of Y box binding-1 (YB-1), which is a member of a family of DNA-binding proteins that contain a cold shock domain, plays a significant role in the acquisition of drug resistance by upregulating expression of the multidrug resistance-1 (MDR-1) gene product, p-glycoprotein. The tumor suppressor protein p53 is thought to be essential for nuclear translocation of YB-1. We hypothesized that nuclear translocation of YB-1 might be associated with drug resistance of bladder cancer with an abnormality of the TP53 gene that results in a mutated p53 protein. To test this hypothesis, we analyzed the association of YB-1 with drug resistance of TP53-mutated bladder cancer, including immunohistochemical analysis of YB-1, p-glycoprotein and p53 in vivo as well as the function of YB-1 nuclear translocation and regulation of its translocation by p53 in vitro. Additionally, we examined the association between the nuclear translocation of YB-1 and gemcitabine, a major anticancer-drug for bladder cancer, in cancer cell lines. Nuclear expression of YB-1 was correlated with the expression of p-glycoprotein and p53 in bladder cancer cases (p<0.05). In vitro, both introduction of TP53 and gemcitabine induced nuclear translocation of YB-1. These data indicate that YB-1 translocates to the nucleus coordinately with p53 expression and is involved in gemcitabine resistance in bladder cancer. Nuclear expression of YB-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yamashita T, Higashi M, Momose S, Morozumi M and Tamaru J: Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer. Int J Oncol 51: 579-586, 2017.
APA
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., & Tamaru, J. (2017). Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer. International Journal of Oncology, 51, 579-586. https://doi.org/10.3892/ijo.2017.4031
MLA
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., Tamaru, J."Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer". International Journal of Oncology 51.2 (2017): 579-586.
Chicago
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., Tamaru, J."Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer". International Journal of Oncology 51, no. 2 (2017): 579-586. https://doi.org/10.3892/ijo.2017.4031
Copy and paste a formatted citation
x
Spandidos Publications style
Yamashita T, Higashi M, Momose S, Morozumi M and Tamaru J: Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer. Int J Oncol 51: 579-586, 2017.
APA
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., & Tamaru, J. (2017). Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer. International Journal of Oncology, 51, 579-586. https://doi.org/10.3892/ijo.2017.4031
MLA
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., Tamaru, J."Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer". International Journal of Oncology 51.2 (2017): 579-586.
Chicago
Yamashita, T., Higashi, M., Momose, S., Morozumi, M., Tamaru, J."Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer". International Journal of Oncology 51, no. 2 (2017): 579-586. https://doi.org/10.3892/ijo.2017.4031
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