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Article

Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model

  • Authors:
    • Naoto Katayama
    • Koji Sugimoto
    • Takuya Okada
    • Takeshi Ueha
    • Yoshitada Sakai
    • Hideo Akiyoshi
    • Keiichiro Mie
    • Eisuke Ueshima
    • Keitaro Sofue
    • Yutaka Koide
    • Ryuichiro Tani
    • Tomoyuki Gentsu
    • Masato Yamaguchi
  • View Affiliations / Copyright

    Affiliations: Department of Radiology and Center for Endovascular Therapy, Kobe University Hospital, Chuo-ku, Kobe 650-0017, Japan, Division of Rehabilitation Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan, Laboratory of Veterinary Surgery, Department of Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
  • Pages: 695-701
    |
    Published online on: June 26, 2017
       https://doi.org/10.3892/ijo.2017.4056
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Abstract

The present study aimed to evaluate the efficacy of an intra-arterially infused carbon dioxide (CO2)-saturated solution in sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. Forty VX2 liver tumor-bearing Japanese white rabbits were randomly divided into four groups and infused via the proper hepatic artery with a saline solution (control group), CO2-saturated solution (CO2 group), cisplatin solution (cisplatin group), or CO2-saturated solution and cisplatin solution (combined group). The tumor volume (TV) and the relative tumor volume (RTV), RTV = (TV on day 3 or 7)/(TV on day 0) x 100, were calculated using contrast-enhanced computed tomography. Hypoxia-inducible factor-1α (HIF‑1α) and carbonic anhydrase IX (CA IX) staining were used to evaluate cellular hypoxia. Cleaved caspase-3 and cleaved caspase-9 were analyzed to assess tumor apoptosis. The mean RTV on days 3 and 7 were 202.6±23.7 and 429.2±94.8%, respectively, in the control group; 172.2±38.1 and 376.5±61.1% in the CO2 group; 156.1±15.1 and 269.6±45.2% in the cisplatin group; and 118.3±28.1 and 210.3±55.1% in the combined group. RTV was significantly lower in the CO2 group than in the control group (day 3; P<0.05), and in the combined group than in the cisplatin group (days 3 and 7; P<0.05). HIF-1α and CA IX suppression, and increased cleaved caspase-3 and cleaved caspase-9 expression, were detected in the CO2 and combined groups, compared with the other two groups. An intra-arterially infused CO2-saturated solution inhibits liver VX2 tumor growth and sensitizes the anticancer effect of cisplatin.
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Copy and paste a formatted citation
Spandidos Publications style
Katayama N, Sugimoto K, Okada T, Ueha T, Sakai Y, Akiyoshi H, Mie K, Ueshima E, Sofue K, Koide Y, Koide Y, et al: Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. Int J Oncol 51: 695-701, 2017.
APA
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H. ... Yamaguchi, M. (2017). Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. International Journal of Oncology, 51, 695-701. https://doi.org/10.3892/ijo.2017.4056
MLA
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H., Mie, K., Ueshima, E., Sofue, K., Koide, Y., Tani, R., Gentsu, T., Yamaguchi, M."Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model". International Journal of Oncology 51.2 (2017): 695-701.
Chicago
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H., Mie, K., Ueshima, E., Sofue, K., Koide, Y., Tani, R., Gentsu, T., Yamaguchi, M."Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model". International Journal of Oncology 51, no. 2 (2017): 695-701. https://doi.org/10.3892/ijo.2017.4056
Copy and paste a formatted citation
x
Spandidos Publications style
Katayama N, Sugimoto K, Okada T, Ueha T, Sakai Y, Akiyoshi H, Mie K, Ueshima E, Sofue K, Koide Y, Koide Y, et al: Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. Int J Oncol 51: 695-701, 2017.
APA
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H. ... Yamaguchi, M. (2017). Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. International Journal of Oncology, 51, 695-701. https://doi.org/10.3892/ijo.2017.4056
MLA
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H., Mie, K., Ueshima, E., Sofue, K., Koide, Y., Tani, R., Gentsu, T., Yamaguchi, M."Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model". International Journal of Oncology 51.2 (2017): 695-701.
Chicago
Katayama, N., Sugimoto, K., Okada, T., Ueha, T., Sakai, Y., Akiyoshi, H., Mie, K., Ueshima, E., Sofue, K., Koide, Y., Tani, R., Gentsu, T., Yamaguchi, M."Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model". International Journal of Oncology 51, no. 2 (2017): 695-701. https://doi.org/10.3892/ijo.2017.4056
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