Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway

  • Authors:
    • Yukie Takahashi
    • Chizu Tanikawa
    • Takafumi Miyamoto
    • Makoto Hirata
    • Guanxiong Wang
    • Koji Ueda
    • Tsunehiko Komatsu
    • Koichi Matsuda
  • View Affiliations

  • Published online on: June 28, 2017     https://doi.org/10.3892/ijo.2017.4060
  • Pages: 724-736
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

p53, one of the most frequently mutated genes in colon cancer, suppresses cancer development through transactivation of its targets. Herein, we conducted a comprehensive analysis of the p53 downstream pathway in colorectal cancer by using multi-omics analysis. Mass spectrometric analysis of HCT116 p53+/+ and HCT116 p53-/- cells treated with adriamycin identified 124 proteins increased by DNA damage in a p53-dependent manner. Further screening using a cDNA microarray and the TCGA database revealed MICALL1 as a novel p53 target, and we identified functional p53 binding motifs located approximately 3000 base pairs upstream of the MICALL1 gene. MICALL1 expression was significantly decreased in colorectal cancer tissues with p53 mutation compared with those without p53 mutation. In response to DNA damage, MICALL1 co-localized with RAB8A and CD2AP at tubular recycling endosomes, whereas these proteins hardly localized at tubular recycling endosomes when p53 or MICALL1 expression was inhibited by siRNA. Our findings show that p53 regulates tubular recycling endosome biogenesis via transcriptional regulation of MICALL1, whose expression is frequently suppressed in colorectal cancer tissues.
View Figures
View References

Related Articles

Journal Cover

August-2017
Volume 51 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi Y, Tanikawa C, Miyamoto T, Hirata M, Wang G, Ueda K, Komatsu T and Matsuda K: Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. Int J Oncol 51: 724-736, 2017
APA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K. ... Matsuda, K. (2017). Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. International Journal of Oncology, 51, 724-736. https://doi.org/10.3892/ijo.2017.4060
MLA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51.2 (2017): 724-736.
Chicago
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51, no. 2 (2017): 724-736. https://doi.org/10.3892/ijo.2017.4060