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Article

Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway

  • Authors:
    • Yukie Takahashi
    • Chizu Tanikawa
    • Takafumi Miyamoto
    • Makoto Hirata
    • Guanxiong Wang
    • Koji Ueda
    • Tsunehiko Komatsu
    • Koichi Matsuda
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan, Laboratory of Clinical Genome Sequencing, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan, Cancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan, Department of Hematology, Teikyo University Chiba Medical Center, Chiba, Japan
  • Pages: 724-736
    |
    Published online on: June 28, 2017
       https://doi.org/10.3892/ijo.2017.4060
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Abstract

p53, one of the most frequently mutated genes in colon cancer, suppresses cancer development through transactivation of its targets. Herein, we conducted a comprehensive analysis of the p53 downstream pathway in colorectal cancer by using multi-omics analysis. Mass spectrometric analysis of HCT116 p53+/+ and HCT116 p53-/- cells treated with adriamycin identified 124 proteins increased by DNA damage in a p53-dependent manner. Further screening using a cDNA microarray and the TCGA database revealed MICALL1 as a novel p53 target, and we identified functional p53 binding motifs located approximately 3000 base pairs upstream of the MICALL1 gene. MICALL1 expression was significantly decreased in colorectal cancer tissues with p53 mutation compared with those without p53 mutation. In response to DNA damage, MICALL1 co-localized with RAB8A and CD2AP at tubular recycling endosomes, whereas these proteins hardly localized at tubular recycling endosomes when p53 or MICALL1 expression was inhibited by siRNA. Our findings show that p53 regulates tubular recycling endosome biogenesis via transcriptional regulation of MICALL1, whose expression is frequently suppressed in colorectal cancer tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Takahashi Y, Tanikawa C, Miyamoto T, Hirata M, Wang G, Ueda K, Komatsu T and Matsuda K: Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. Int J Oncol 51: 724-736, 2017.
APA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K. ... Matsuda, K. (2017). Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. International Journal of Oncology, 51, 724-736. https://doi.org/10.3892/ijo.2017.4060
MLA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51.2 (2017): 724-736.
Chicago
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51, no. 2 (2017): 724-736. https://doi.org/10.3892/ijo.2017.4060
Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi Y, Tanikawa C, Miyamoto T, Hirata M, Wang G, Ueda K, Komatsu T and Matsuda K: Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. Int J Oncol 51: 724-736, 2017.
APA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K. ... Matsuda, K. (2017). Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway. International Journal of Oncology, 51, 724-736. https://doi.org/10.3892/ijo.2017.4060
MLA
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51.2 (2017): 724-736.
Chicago
Takahashi, Y., Tanikawa, C., Miyamoto, T., Hirata, M., Wang, G., Ueda, K., Komatsu, T., Matsuda, K."Regulation of tubular recycling endosome biogenesis by the p53-MICALL1 pathway". International Journal of Oncology 51, no. 2 (2017): 724-736. https://doi.org/10.3892/ijo.2017.4060
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