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Article

Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells

  • Authors:
    • Kyoung Ah Kang
    • Mei Jing Piao
    • Yea Seong Ryu
    • Yu Jae Hyun
    • Jeong Eon Park
    • Kristina Shilnikova
    • Ao Xuan Zhen
    • Hee Kyoung Kang
    • Young Sang Koh
    • Yong Joo Jeong
    • Jin Won Hyun
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, School of Medicine and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea, Department of Bio and Nanochemistry, Kookmin University, Seoul 02707, Republic of Korea
  • Pages: 1169-1178
    |
    Published online on: August 2, 2017
       https://doi.org/10.3892/ijo.2017.4091
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Abstract

The present study determined whether luteolin induces HT-29 colon cancer cell death through an antioxidant effect such as the activation of antioxidant enzymes. Luteolin decreased cell viability in human colon cancer cells (HT-29), whereas it had no effect on normal colon cells (FHC). Luteolin induced apoptosis by activating the mitochondria-mediated caspase pathway in HT-29 cells. Luteolin caused loss of the mitochondrial membrane action potential, increased mitochondrial Ca2+ level, upregulated Bax, downregulated Bcl-2, induced the release of cytochrome c from mitochondria to the cytosol, and increased the levels of the active forms of caspase-9 and caspase-3. Luteolin-induced apoptosis was accompanied by the activation of intracellular and mitochondrial reactive oxygen species scavenging through the activation of antioxidant enzymes, such as superoxide dismutase and catalase in HT-29 cells. Luteolin increased the level of reduced glutathione (GSH) and the expression of GSH synthetase, which catalyzes the second step of GSH biosynthesis. The apoptotic effect of luteolin was mediated by the activation of the mitogen-activated protein kinase signaling pathway. The present results indicate that luteolin induces apoptosis by promoting antioxidant activity and activating MAPK signaling in human colon cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kang KA, Piao MJ, Ryu YS, Hyun YJ, Park JE, Shilnikova K, Zhen AX, Kang HK, Koh YS, Jeong YJ, Jeong YJ, et al: Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells. Int J Oncol 51: 1169-1178, 2017.
APA
Kang, K.A., Piao, M.J., Ryu, Y.S., Hyun, Y.J., Park, J.E., Shilnikova, K. ... Hyun, J.W. (2017). Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells. International Journal of Oncology, 51, 1169-1178. https://doi.org/10.3892/ijo.2017.4091
MLA
Kang, K. A., Piao, M. J., Ryu, Y. S., Hyun, Y. J., Park, J. E., Shilnikova, K., Zhen, A. X., Kang, H. K., Koh, Y. S., Jeong, Y. J., Hyun, J. W."Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells". International Journal of Oncology 51.4 (2017): 1169-1178.
Chicago
Kang, K. A., Piao, M. J., Ryu, Y. S., Hyun, Y. J., Park, J. E., Shilnikova, K., Zhen, A. X., Kang, H. K., Koh, Y. S., Jeong, Y. J., Hyun, J. W."Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells". International Journal of Oncology 51, no. 4 (2017): 1169-1178. https://doi.org/10.3892/ijo.2017.4091
Copy and paste a formatted citation
x
Spandidos Publications style
Kang KA, Piao MJ, Ryu YS, Hyun YJ, Park JE, Shilnikova K, Zhen AX, Kang HK, Koh YS, Jeong YJ, Jeong YJ, et al: Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells. Int J Oncol 51: 1169-1178, 2017.
APA
Kang, K.A., Piao, M.J., Ryu, Y.S., Hyun, Y.J., Park, J.E., Shilnikova, K. ... Hyun, J.W. (2017). Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells. International Journal of Oncology, 51, 1169-1178. https://doi.org/10.3892/ijo.2017.4091
MLA
Kang, K. A., Piao, M. J., Ryu, Y. S., Hyun, Y. J., Park, J. E., Shilnikova, K., Zhen, A. X., Kang, H. K., Koh, Y. S., Jeong, Y. J., Hyun, J. W."Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells". International Journal of Oncology 51.4 (2017): 1169-1178.
Chicago
Kang, K. A., Piao, M. J., Ryu, Y. S., Hyun, Y. J., Park, J. E., Shilnikova, K., Zhen, A. X., Kang, H. K., Koh, Y. S., Jeong, Y. J., Hyun, J. W."Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells". International Journal of Oncology 51, no. 4 (2017): 1169-1178. https://doi.org/10.3892/ijo.2017.4091
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