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Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy

  • Authors:
    • Yong Tao
    • Yunsheng Ou
    • Hang Yin
    • Yanyang Chen
    • Shenxi Zhong
    • Yongjian Gao
    • Zenghui Zhao
    • Bin He
    • Qiu Huang
    • Qianxing Deng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China, Department of Orthopedics, People's Hosipital of Leshan, Leshan, Sichuan 614000, P.R. China, Department of Orthopedics, People's Hospital of Fengdu, Fengdu, Chongqing 408200, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1427-1438
    |
    Published online on: September 27, 2017
       https://doi.org/10.3892/ijo.2017.4136
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Abstract

The present study was performed to establish and characterize new human osteosarcoma cell lines resistant to pyropheophorbide-α methyl ester‑mediated photodynamic therapy (MPPa-PDT). MPPa-PDT-resistant cells are isolated from the human osteosarcoma MG63 and HOS cell lines and two resistant populations were finally acquired, including MG63/PDT and HOS/PDT. Cell Counting Kit-8 (CCK-8) assay was used to determine the MPPa-PDT, cisplatin (CDDP) resistance and proliferation of MG63, MG63/PDT, HOS and HOS/PDT cells. The intracellular ROS were analyzed using DCFH-DA staining. The colony formation, invasion and migration of parental and resistant cells were compared. FCM was employed to examine the cell cycle distribution, the apoptosis rate and the proportion of CD133+ cells. The fluorescence intensity of intracellular MPPa was observed by fluorescence microscopy and quantified using microplate reader. The protein levels were assessed by western blotting (WB). Compared with two parental cells, MG63/PDT and HOS/PDT were 1.67- and 1.61-fold resistant to MPPa-PDT, respectively, and also exhibited the resistance to CDDP. FCM assays confirmed that both MG63/PDT and HOS/PDT cells treated with MPPa-PDT displayed a significantly lower apoptosis rate in comparison with their corresponding parental cells. The expression of apoptosis-related proteins (i.e. cleaved-caspase 3 and cleaved‑PARP), intracellular ROS and the antioxidant proteins (HO-1 and SOD1) in MG63/PDT and HOS/PDT cells was also lower than that in parental cells. Both MG63/PDT and HOS/PDT cells exhibited changes in proliferation, photosensitizer absorption, colony formation, invasion, migration and the cell cycle distribution as compared to MG63 and HOS cells, respectively. Compared to MG63 and HOS cells, both resistant cell lines had a higher expression of CD133, survivin, Bcl-xL, Bcl-2, MRP1, MDR1 and ABCG2, but a lower expression of Bax. The present study successfully established two resistant human osteosarcoma cell lines which are valuable to explore the resistance-related mechanisms and the approaches to overcome resistance.
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View References

1 

Wang W, Yang J, Wang Y, Wang D, Han G, Jia J, Xu M and Bi W: Survival and prognostic factors in Chinese patients with osteosarcoma: 13-year experience in 365 patients treated at a single institution. Pathol Res Pract. 213:119–125. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Whelan J, McTiernan A, Cooper N, Wong YK, Francis M, Vernon S and Strauss SJ: Incidence and survival of malignant bone sarcomas in England 1979–2007. Int J Cancer. 131:E508–E517. 2012. View Article : Google Scholar

3 

Huang Q, Ou YS, Tao Y, Yin H and Tu PH: Apoptosis and autophagy induced by pyropheophorbide-α methyl ester-mediated photodynamic therapy in human osteosarcoma MG-63 cells. Apoptosis. 21:749–760. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Agostinis P, Berg K, Cengel KA, Foster TH, Girotti AW, Gollnick SO, Hahn SM, Hamblin MR, Juzeniene A, Kessel D, et al: Photodynamic therapy of cancer: An update. CA Cancer J Clin. 61:250–281. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Khaled YS, Wright KE, Melcher A and Jayne D: Anti-cancer effects of oncolytic viral therapy combined with photodynamic therapy in human pancreatic cancer cell lines. Spring Meeting for Clinician Scientists in Training 2015. 26–Feb. 2015, https://doi.org/10.1016/S0140-6736(15)60371-3.

6 

Cheng Y, Chang Y, Feng Y, Liu N, Sun X, Feng Y, Li X and Zhang H: Simulated sunlight-mediated photodynamic therapy for melanoma skin cancer by titanium-dioxide-nanoparticle-gold-nanocluster-graphene heterogeneous nanocomposites. Small. 13:16039352017. View Article : Google Scholar

7 

Kuzyniak W, Schmidt J, Glac W, Berkholz J, Steinemann G, Hoffmann B, Ermilov EA, Gürek AG, Ahsen V, Nitzsche B, et al: Novel zinc phthalocyanine as a promising photosensitizer for photodynamic treatment of esophageal cancer. Int J Oncol. 50:953–963. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Yano T, Kasai H, Horimatsu T, Yoshimura K, Teramukai S, Morita S, Tada H, Yamamoto Y, Kataoka H, Kakushima N, et al: A multicenter phase II study of salvage photodynamic therapy using talaporfin sodium (ME2906) and a diode laser (PNL6405EPG) for local failure after chemoradiotherapy or radiotherapy for esophageal cancer. Oncotarget. 8:22135–22144. 2017.PubMed/NCBI

9 

Luo T, Wilson BC and Lu QB: Evaluation of one- and two-photon activated photodynamic therapy with pyropheophorbide-a methyl ester in human cervical, lung and ovarian cancer cells. J Photochem Photobiol B. 132:102–110. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Li KM, Sun X, Koon HK, Leung WN, Fung MC, Wong RN, Lung ML, Chang CK and Mak NK: Apoptosis and expression of cytokines triggered by pyropheophorbide-a methyl ester-mediated photodynamic therapy in nasopharyngeal carcinoma cells. Photodiagn Photodyn Ther. 3:247–258. 2006. View Article : Google Scholar

11 

Tian Y, Leung W, Yue K and Mak N: Cell death induced by MPPa-PDT in prostate carcinoma in vitro and in vivo. Biochem Biophys Res Commun. 348:413–420. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Tian YY, Hu XY, Leung WN, Yuan HQ, Zhang LY, Cui FA and Tian X: Investigation of photodynamic effect caused by MPPa-PDT on breast cancer Investigation of photodynamic effect caused by MPPa-PDT. Laser Phys Lett. 9:754–758. 2012. View Article : Google Scholar

13 

Gilaberte Y, Milla L, Salazar N, Vera-Alvarez J, Kourani O, Damian A, Rivarola V, Roca MJ, Espada J, González S, et al: Cellular intrinsic factors involved in the resistance of squamous cell carcinoma to photodynamic therapy. J Invest Dermatol. 134:2428–2437. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Zamarrón A, Lucena SR, Salazar N, Sanz-Rodríguez F, Jaén P, Gilaberte Y, González S and Juarranz Á: Isolation and charac-terization of PDT-resistant cancer cells. Photochem Photobiol Sci. 14:1378–1389. 2015. View Article : Google Scholar

15 

Anderson SJ, Wapnir I, Dignam JJ, Fisher B, Mamounas EP, Jeong JH, Geyer CE Jr, Wickerham DL, Costantino JP and Wolmark N: Prognosis after ipsilateral breast tumor recurrence and locoregional recurrences in patients treated by breast-conserving therapy in five National Surgical Adjuvant Breast and Bowel Project protocols of node-negative breast cancer. J Clin Oncol. 27:2466–2473. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Wang C, Guo LB, Ma JY, Li YM and Liu HM: Establishment and characterization of a paclitaxel-resistant human esophageal carcinoma cell line. Int J Oncol. 43:1607–1617. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Jeon M, Rahman N and Kim YS: Cytoprotective effect of Makgeolli lees on paraquat induced oxidative stress in A549 cells via activation of NRF2 and antioxidant genes. J Microbiol Biotechnol. 26:277–286. 2016. View Article : Google Scholar

18 

Dean M: ABC transporters, drug resistance, and cancer stem cells. J Mammary Gland Biol Neoplasia. 14:3–9. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Zhang B, Pan JS, Liu JY, Han SP, Hu G and Wang B: Effects of chemotherapy and/or radiotherapy on survivin expression in ovarian cancer. Methods Find Exp Clin Pharmacol. 28:619–625. 2006. View Article : Google Scholar

20 

Czabotar PE, Lessene G, Strasser A and Adams JM: Control of apoptosis by the BCL-2 protein family: Implications for physiology and therapy. Nat Rev Mol Cell Biol. 15:49–63. 2014. View Article : Google Scholar

21 

Restifo NP, Smyth MJ and Snyder A: Acquired resistance to immunotherapy and future challenges. Nat Rev Cancer. 16:121–126. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Wicki A, Mandalà M, Massi D, Taverna D, Tang H, Hemmings BA and Xue G: Acquired resistance to clinical cancer therapy: A Twist in physiological signaling. Physiol Rev. 96:805–829. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Solyanik GI: Multifactorial nature of tumor drug resistance. Exp Oncol. 32:181–185. 2010.

24 

Milla LN, Cogno IS, Rodríguez ME, Sanz-Rodríguez F, Zamarrón A, Gilaberte Y, Carrasco E, Rivarola VA and Juarranz A: Isolation and characterization of squamous carcinoma cells resistant to photodynamic therapy. J Cell Biochem. 112:2266–2278. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Tu P, Huang Q, Ou Y, Du X, Li K, Tao Y and Yin H: Aloe-emodin-mediated photodynamic therapy induces autophagy and apoptosis in human osteosarcoma cell line MG-63 through the ROS/JNK signaling pathway. Oncol Rep. 35:3209–3215. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Chau LY: Heme oxygenase-1: Emerging target of cancer therapy. J Biomed Sci. 22:222015. View Article : Google Scholar : PubMed/NCBI

27 

Furfaro AL, Traverso N, Domenicotti C, Piras S, Moretta L, Marinari UM, Pronzato MA and Nitti M: The Nrf2/HO-1 axis in cancer cell growth and chemoresistance. Oxid Med Cell Longev. 2016:19581742016. View Article : Google Scholar

28 

Ciesla M, Marona P, Kozakowska M, Jez M, Seczynska M, Loboda A, Bukowska-Strakova K, Szade A, Walawender M, Kusior M, et al: Heme oxygenase-1 controls an HDAC4-miR-206 pathway of oxidative stress in rhabdomyosarcoma. Cancer Res. 76:5707–5718. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Lv X, Song DM, Niu YH and Wang BS: Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin. Apoptosis. 21:489–501. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Soares HT, Campos JR, Gomes-da-Silva LC, Schaberle FA, Dąbrowski JM and Arnaut LG: Pro-oxidant and antioxidant effects in Photodynamic Therapy: Cells recognize that not all exogenous ROS are alike. ChemBioChem. 17:836–842. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Wright KE, MacRobert AJ and Phillips JB: Inhibition of specific cellular antioxidant pathways increases the sensitivity of neurons to meta-tetrahydroxyphenyl chlorin-mediated photodynamic therapy in a 3D co-culture model. Photochem Photobiol. 88:1539–1545. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Tian S, Yong M, Zhu J, Zhang L, Pan L, Chen Q, Li KT, Kong YH, Jiang Y, Yu TH, et al: Enhancement of the effect of methyl pyropheophorbide-a-mediated photodynamic therapy was achieved by increasing ROS via inhibition of Nrf2-HO-1 or Nrf2-ABCG2 signaling. Anticancer Agents Med Chem. 17:12017. View Article : Google Scholar

33 

Ishikawa T, Nakagawa H, Hagiya Y, Nonoguchi N, Miyatake S and Kuroiwa T: Key role of human ABC transporter ABCG2 in photodynamic therapy and photodynamic diagnosis. Adv Pharmacol Sci. 2010:5873062010.PubMed/NCBI

34 

Li C, Guo D, Tang B, Zhang Y, Zhang K and Nie L: Notch1 is associated with the multidrug resistance of hypoxic osteosarcoma by regulating MRP1 gene expression. Neoplasma. 63:734–742. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Liu T, Li Z, Zhang Q, De Amorim Bernstein K, Lozano-Calderon S, Choy E, Hornicek FJ and Duan Z: Targeting ABCB1 (MDR1) in multi-drug resistant osteosarcoma cells using the CRISPR-Cas9 system to reverse drug resistance. Oncotarget. 7:83502–83513. 2016.PubMed/NCBI

36 

Ferrario A, Rucker N, Wong S, Luna M and Gomer CJ: Survivin, a member of the inhibitor of apoptosis family, is induced by photodynamic therapy and is a target for improving treatment response. Cancer Res. 67:4989–4995. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Otto T and Sicinski P: Cell cycle proteins as promising targets in cancer therapy. Nat Rev Cancer. 17:93–115. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Han T, Zhu X, Wang J, Zhao H, Ma Q, Zhao J, Qiu X and Fan Q: Establishment and characterization of a cisplatin-resistant human osteosarcoma cell line. Oncol Rep. 32:1133–1139. 2014.PubMed/NCBI

39 

Baldea I, Olteanu DE, Bolfa P, Tabaran F, Ion RM and Filip GA: Melanogenesis and DNA damage following photodynamic therapy in melanoma with two meso-substituted porphyrins. J Photochem Photobiol B. 161:402–410. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Borgstahl GEO, Brader K, Mosel A, Liu S, Kremmer E, Goettsch KA, Kolar C, Nasheuer HP and Oakley GG: Interplay of DNA damage and cell cycle signaling at the level of human replication protein A. DNA Repair. 21:12–23. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Casas A, Di Venosa G, Vanzulli S, Perotti C, Mamome L, Rodriguez L, Simian M, Juarranz A, Pontiggia O, Hasan T, et al: Decreased metastatic phenotype in cells resistant to aminolevulinic acid-photodynamic therapy. Cancer Lett. 271:342–351. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Stuckey DW and Shah K: Stem cell-based therapies for cancer treatment: Separating hope from hype. Nat Rev Cancer. 14:683–691. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Tirino V, Desiderio V, d'Aquino R, De Francesco F, Pirozzi G, Graziano A, Galderisi U, Cavaliere C, De Rosa A, Papaccio G, et al: Detection and characterization of CD133+ cancer stem cells in human solid tumours. PLoS One. 3:e34692008. View Article : Google Scholar

44 

Veselska R, Hermanova M, Loja T, Chlapek P, Zambo I, Vesely K, Zitterbart K and Sterba J: Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines. BMC Cancer. 8:3002008. View Article : Google Scholar : PubMed/NCBI

45 

He A, Qi W, Huang Y, Feng T, Chen J, Sun Y, Shen Z and Yao Y: CD133 expression predicts lung metastasis and poor prognosis in osteosarcoma patients: A clinical and experimental study. Exp Ther Med. 4:435–441. 2012. View Article : Google Scholar : PubMed/NCBI

46 

Li J, Zhong XY, Li ZY, Cai JF, Zou L, Li JM, Yang T and Liu W: CD133 expression in osteosarcoma and derivation of CD133+ cells. Mol Med Rep. 7:577–584. 2013. View Article : Google Scholar

47 

Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C and De Maria R: Identification and expansion of human colon-cancer-initiating cells. Nature. 445:111–115. 2007. View Article : Google Scholar

48 

Robey RW, Polgar O, Deeken J, To KW and Bates SE: ABCG2: Determining its relevance in clinical drug resistance. Cancer Metastasis Rev. 26:39–57. 2007. View Article : Google Scholar : PubMed/NCBI

49 

Ding XW, Wu JH and Jiang CP: ABCG2: A potential marker of stem cells and novel target in stem cell and cancer therapy. Life Sci. 86:631–637. 2010. View Article : Google Scholar : PubMed/NCBI

50 

Di Fiore R, Santulli A, Ferrante RD, Giuliano M, De Blasio A, Messina C, Pirozzi G, Tirino V, Tesoriere G and Vento R: Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment. J Cell Physiol. 219:301–313. 2009. View Article : Google Scholar : PubMed/NCBI

51 

Jaggupilli A and Elkord E: Significance of CD44 and CD24 as cancer stem cell markers: An enduring ambiguity. Clin Dev Immunol. 2012:7080362012. View Article : Google Scholar : PubMed/NCBI

52 

Spring BQ, Rizvi I, Xu N and Hasan T: The role of photodynamic therapy in overcoming cancer drug resistance. Photochem Photobiol Sci. 14:1476–1491. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tao Y, Ou Y, Yin H, Chen Y, Zhong S, Gao Y, Zhao Z, He B, Huang Q, Deng Q, Deng Q, et al: Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy. Int J Oncol 51: 1427-1438, 2017.
APA
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y. ... Deng, Q. (2017). Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy. International Journal of Oncology, 51, 1427-1438. https://doi.org/10.3892/ijo.2017.4136
MLA
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y., Zhao, Z., He, B., Huang, Q., Deng, Q."Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy". International Journal of Oncology 51.5 (2017): 1427-1438.
Chicago
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y., Zhao, Z., He, B., Huang, Q., Deng, Q."Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy". International Journal of Oncology 51, no. 5 (2017): 1427-1438. https://doi.org/10.3892/ijo.2017.4136
Copy and paste a formatted citation
x
Spandidos Publications style
Tao Y, Ou Y, Yin H, Chen Y, Zhong S, Gao Y, Zhao Z, He B, Huang Q, Deng Q, Deng Q, et al: Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy. Int J Oncol 51: 1427-1438, 2017.
APA
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y. ... Deng, Q. (2017). Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy. International Journal of Oncology, 51, 1427-1438. https://doi.org/10.3892/ijo.2017.4136
MLA
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y., Zhao, Z., He, B., Huang, Q., Deng, Q."Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy". International Journal of Oncology 51.5 (2017): 1427-1438.
Chicago
Tao, Y., Ou, Y., Yin, H., Chen, Y., Zhong, S., Gao, Y., Zhao, Z., He, B., Huang, Q., Deng, Q."Establishment and characterization of human osteosarcoma cells resistant to pyropheophorbide-α methyl ester-mediated photodynamic therapy". International Journal of Oncology 51, no. 5 (2017): 1427-1438. https://doi.org/10.3892/ijo.2017.4136
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