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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells

  • Authors:
    • Masanori Kawano
    • Kazuhiro Tanaka
    • Ichiro Itonaga
    • Tatsuya Iwasaki
    • Hiroshi Tsumura
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Faculty of Medicine, Oita University, Oita 879-5593, Japan
  • Pages: 1842-1850
    |
    Published online on: October 12, 2017
       https://doi.org/10.3892/ijo.2017.4155
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Abstract

Transforming growth factor-β receptor II (TGFBR2) is implicated in various types of cancer. Most molecules involved in the TGF-β pathway can be degraded by one or more microRNAs (miRNAs). In the present study, we show that miRNA plays an important role in downregulating TGFBR2 expression in Ewing's sarcoma (ES) cells. Microarray-based analyses revealed that the expression of miR-20b was significantly increased, whereas TGFBR2 and MYC were significantly downregulated and upregulated, respectively, in all ES cells compared to their expression in human mesenchymal stem cells (hMSCs). In ES cell lines, anti-miR-20b increased TGFBR2 expression and significantly decreased MYC expression, showing an inverse relationship with TGFBR2. The induction by anti-miR-20b further prohibited ES cell growth and cell cycle progression. Moreover, decreased miR-20b in ES cells significantly inhibited tumor growth in vivo. Taken together, these results suggest that miR-20b behaves as an oncogene in ES when its overexpression is unregulated by targeting TGFBR2. Because downstream TGFBR2 and TGF-β signaling regulate cell cycle, apoptosis, and tumor proliferation via MYC, our findings may contribute to new targeted therapies for ES.
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Copy and paste a formatted citation
Spandidos Publications style
Kawano M, Tanaka K, Itonaga I, Iwasaki T and Tsumura H: MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells. Int J Oncol 51: 1842-1850, 2017.
APA
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., & Tsumura, H. (2017). MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells. International Journal of Oncology, 51, 1842-1850. https://doi.org/10.3892/ijo.2017.4155
MLA
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., Tsumura, H."MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells". International Journal of Oncology 51.6 (2017): 1842-1850.
Chicago
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., Tsumura, H."MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells". International Journal of Oncology 51, no. 6 (2017): 1842-1850. https://doi.org/10.3892/ijo.2017.4155
Copy and paste a formatted citation
x
Spandidos Publications style
Kawano M, Tanaka K, Itonaga I, Iwasaki T and Tsumura H: MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells. Int J Oncol 51: 1842-1850, 2017.
APA
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., & Tsumura, H. (2017). MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells. International Journal of Oncology, 51, 1842-1850. https://doi.org/10.3892/ijo.2017.4155
MLA
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., Tsumura, H."MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells". International Journal of Oncology 51.6 (2017): 1842-1850.
Chicago
Kawano, M., Tanaka, K., Itonaga, I., Iwasaki, T., Tsumura, H."MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells". International Journal of Oncology 51, no. 6 (2017): 1842-1850. https://doi.org/10.3892/ijo.2017.4155
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