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Article

GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells

  • Authors:
    • Kunping Liu
    • Jinbang Li
    • Xuefang Wu
    • Meixiang Chen
    • Feng Luo
    • Jun Li
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, Guangdong 511518, P.R. China, Department of General Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 1821-1830
    |
    Published online on: October 16, 2017
       https://doi.org/10.3892/ijo.2017.4163
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Abstract

Multi-targets inhibitor 6-bromo-indirubin-3'-oxime (BIO) has diverse biological effects on cancer cells. The key component of the β-catenin destruction complex glycogen synthase kinase 3β (GSK-3β), one of the major target for BIO, polyubiquitination and degradation of the main oncoprotein β-catenin in colorectal cancer (CRC). In the present study, we evaluated the effect of BIO on drug resistance and biological properties of CRC cells. Whole-genome transcriptional profiling revealed that differentially expressed genes were mainly centered on well-characterized signaling pathways including stem cell, cell adhesion and cell growth in BIO-treated CRC cells. BIO treatment downregulated migration and invasion abilities of CRC cells, accompanying with MMP-9 downregulated and E-cadherin upregulated CRC cells. BIO treatment decreased apoptosis induced by 5-Fu/DDP in CRC SW480 cells. In addition, BIO treatment reversed the 5-Fu-induced CD133+ cell downregulation trend in CRC SW620 cells. After incubation with BIO, the expression levels of EpCAM, TERT and DCAMKL-1 proteins were upregulated in CRC cells. BIO treatment downregulated the activity of GSK-3β, upregulated and transported β-catenin to the nucleus in CRC cells. Our findings reveal that BIO treatment upregulated stemness, adhesive and chemoresistance of CRC cells. GSK-3β inhibition and WNT/β-catenin activation by BIO, may partly result in the biological behavior alterations in CRC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Liu K, Li J, Wu X, Chen M, Luo F and Li J: GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells. Int J Oncol 51: 1821-1830, 2017.
APA
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., & Li, J. (2017). GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells. International Journal of Oncology, 51, 1821-1830. https://doi.org/10.3892/ijo.2017.4163
MLA
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., Li, J."GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells". International Journal of Oncology 51.6 (2017): 1821-1830.
Chicago
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., Li, J."GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells". International Journal of Oncology 51, no. 6 (2017): 1821-1830. https://doi.org/10.3892/ijo.2017.4163
Copy and paste a formatted citation
x
Spandidos Publications style
Liu K, Li J, Wu X, Chen M, Luo F and Li J: GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells. Int J Oncol 51: 1821-1830, 2017.
APA
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., & Li, J. (2017). GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells. International Journal of Oncology, 51, 1821-1830. https://doi.org/10.3892/ijo.2017.4163
MLA
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., Li, J."GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells". International Journal of Oncology 51.6 (2017): 1821-1830.
Chicago
Liu, K., Li, J., Wu, X., Chen, M., Luo, F., Li, J."GSK-3β inhibitor 6-bromo-indirubin-3'-oxime promotes both adhesive activity and drug resistance in colorectal cancer cells". International Journal of Oncology 51, no. 6 (2017): 1821-1830. https://doi.org/10.3892/ijo.2017.4163
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