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Article

Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells

  • Authors:
    • Vishak Raman
    • Jorge Luis Fuentes Lorenzo
    • Elena E. Stashenko
    • Morris Levy
    • Maria M. Levy
    • Ignacio G. Camarillo
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, Purdue University, West Lafayette, IN, USA, Microbiology and Environmental Mutagenesis Laboratory, School of Biology, Industrial University of Santander, Bucaramanga, Colombia, Research Center for Biomolecules (CIBIMOL), Research Center of Excellence (CENIVAM), Industrial University of Santander, Bucaramanga, Colombia
  • Pages: 1801-1808
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    Published online on: October 19, 2017
       https://doi.org/10.3892/ijo.2017.4169
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Abstract

Treatments targeting hormone receptors typically fail to provide a positive clinical outcome against triple-negative breast cancers (TNBC), which lack expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (Her2/neu). Towards identifying viable treatments for aggressive breast cancer, we have tested an extract of the tropical plant Lippia origanoides (LOE) on TNBC and normal cells lines to uncover its potential anticancer effects. Treatment with LOE reduced TNBC cell viability in a dose-dependent manner to a greater extent than in normal mammary epithelial MCF10A cells. In MDA-MB‑231 cells, LOE was found to halt the cell cycle in the G0/G1 phase via cyclin D1 and cIAP2 regulation, and induce apoptosis without promoting necrosis via caspase-8/-3 and PARP cleavage. Constitutive nuclear factor-κB (NF-κB) signaling has been shown to contribute to the heightened inflammatory state and survival in TNBC cells. Herein, we also provide evidence that LOE inhibits NF-κB signaling by reducing RIP1 protein levels in MDA-MB-231 cells. These studies reveal that LOE suppresses key features of the progression of aggressive breast cancer cells and provides a basis for further definition of its underlying mechanisms of action and anticancer potential.
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Copy and paste a formatted citation
Spandidos Publications style
Raman V, Fuentes Lorenzo JL, Stashenko EE, Levy M, Levy MM and Camarillo IG: Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells. Int J Oncol 51: 1801-1808, 2017.
APA
Raman, V., Fuentes Lorenzo, J.L., Stashenko, E.E., Levy, M., Levy, M.M., & Camarillo, I.G. (2017). Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells. International Journal of Oncology, 51, 1801-1808. https://doi.org/10.3892/ijo.2017.4169
MLA
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51.6 (2017): 1801-1808.
Chicago
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51, no. 6 (2017): 1801-1808. https://doi.org/10.3892/ijo.2017.4169
Copy and paste a formatted citation
x
Spandidos Publications style
Raman V, Fuentes Lorenzo JL, Stashenko EE, Levy M, Levy MM and Camarillo IG: Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells. Int J Oncol 51: 1801-1808, 2017.
APA
Raman, V., Fuentes Lorenzo, J.L., Stashenko, E.E., Levy, M., Levy, M.M., & Camarillo, I.G. (2017). Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells. International Journal of Oncology, 51, 1801-1808. https://doi.org/10.3892/ijo.2017.4169
MLA
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51.6 (2017): 1801-1808.
Chicago
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51, no. 6 (2017): 1801-1808. https://doi.org/10.3892/ijo.2017.4169
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