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Article

RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma

  • Authors:
    • Lei Wang
    • Zhichao Hou
    • Ayshamgul Hasim
    • Abulajiang Abuduerheman
    • Haiping Zhang
    • Madiniyat Niyaz
    • Idiris Awut
    • Halmurat Upur
    • Ilyar Sheyhidin
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Τhe First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China, Department of Pathology, Medical University of Xinjiang, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China, Clinical Medical Research Institute, Τhe First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China, Department of Uyghur Medicine, Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China
  • Pages: 861-871
    |
    Published online on: January 24, 2018
       https://doi.org/10.3892/ijo.2018.4253
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Abstract

Ring finger protein 113A (RNF113A) possesses a C3HC4 zinc finger domain and this domain is found in E3 ubiquitin ligase and is involved in tumorigenesis. To date, and at least to the best of our knowledge, there are no studies available which have investigated RNF113A in cancer. Thus, this study aimed to explore the role of RNF113A in the development of esophageal squamous cell carcinoma (ESCC). For this purpose, paraffin-embedded samples from 117 patients with ESCC were selected, as well as 41 pairs of fresh-frozen ESCC and adjacent normal tissue samples. RNF113A expression was examined by immunohistochemistry and reverse transcription-quantitative PCR (RT-qPCR). RNF113A was overexpressed or silenced in the EC9706 and Eca109 cells. The cells were examined for cell cycle progression, apoptosis, invasiveness and migration. Xenograft tumors were also created in mice using the Eca109 cells. Tumor differentiation (P=0.008) and T classification (P<0.001) were found to be significantly associated with RNF113A expression. No statistically significant association was observed between RNF113A expression and sex, age, histological type, tumor location and lymph node metastasis (N classification). Kaplan-Meier analysis revealed that the patients with ESCC with ahigh expression of RNF113A had a lower survival rate than those with a low expression (P=0.002). Multivariate analysis revealed that RNF113A expression (HR=2.406; 95% CI, 1.301-4.449, P=0.005) was independently associated with overall survival in patients with ESCC. The overexpression of RNF113A promoted proliferation, migration, and invasiveness of ESCC cell lines in vitro, and RNF113A silencing reversed these malignant behaviors. RNF113A knockdown inhibited tumor growth in vivo. Thus, these results indicate that RNF113A promotes the proliferation, migration and invasiveness of ESCC cell lines. RNF113A expression in ESCC is this associated with a poor prognosis of affected patients.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Hou Z, Hasim A, Abuduerheman A, Zhang H, Niyaz M, Awut I, Upur H and Sheyhidin I: RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma. Int J Oncol 52: 861-871, 2018.
APA
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M. ... Sheyhidin, I. (2018). RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma. International Journal of Oncology, 52, 861-871. https://doi.org/10.3892/ijo.2018.4253
MLA
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M., Awut, I., Upur, H., Sheyhidin, I."RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma". International Journal of Oncology 52.3 (2018): 861-871.
Chicago
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M., Awut, I., Upur, H., Sheyhidin, I."RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma". International Journal of Oncology 52, no. 3 (2018): 861-871. https://doi.org/10.3892/ijo.2018.4253
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Hou Z, Hasim A, Abuduerheman A, Zhang H, Niyaz M, Awut I, Upur H and Sheyhidin I: RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma. Int J Oncol 52: 861-871, 2018.
APA
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M. ... Sheyhidin, I. (2018). RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma. International Journal of Oncology, 52, 861-871. https://doi.org/10.3892/ijo.2018.4253
MLA
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M., Awut, I., Upur, H., Sheyhidin, I."RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma". International Journal of Oncology 52.3 (2018): 861-871.
Chicago
Wang, L., Hou, Z., Hasim, A., Abuduerheman, A., Zhang, H., Niyaz, M., Awut, I., Upur, H., Sheyhidin, I."RNF113A promotes the proliferation, migration and invasion, and is associated with a poor prognosis of esophageal squamous cell carcinoma". International Journal of Oncology 52, no. 3 (2018): 861-871. https://doi.org/10.3892/ijo.2018.4253
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