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Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer

  • Authors:
    • Chen Su
    • Daojiang Li
    • Nanpeng Li
    • Yuheng Du
    • Chunxing Yang
    • Yang Bai
    • Changwei Lin
    • Xiaorong Li
    • Yi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China
    Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1479-1490
    |
    Published online on: March 6, 2018
       https://doi.org/10.3892/ijo.2018.4305
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Abstract

Pleomorphic adenoma gene like-2 (PLAGL2) is a zinc finger protein transcription factor, which is upregulated and serves an oncogenic function in multiple human malignancies, including colorectal cancer (CRC). First, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of PLAGL2 in CRC tissues and normal tissues. Then, bioinformatics analysis, RT-qPCR, western blotting, luciferase reporter assays and RNA-binding protein immunoprecipitation assays were performed to explore whether the underlying mechanisms, including copy number variation (CNV), microRNAs (miRNAs/miRs) and RNA-binding proteins (RBPs) led to the abnormal expression of PLAGL2. Finally, cell counting kit-8 assays, Transwell assays and xenograft models were used to detect carcinogenesis-associated characteristics based on the 3'-untranslated region (3'-UTR) of PLAGL2. In the present study, PLAGL2 was revealed to be upregulated in CRC tissues compared with normal CRC tissues. CNV was one of the causes leading to the upregulation of PLAGL2. miRNA, including downregulated miR-486-5p, and RBPs, including upregulated human antigen R (HuR), were other key underlying causes. In addition, PLAGL2 3'-UTR was revealed to promote the progression of CRC in vitro and in vivo, and to regulate the expression of C-MYC and CD44. To conclude, these results suggested that high expression of PLAGL2 in CRC was associated with CNV, miR-486-5p and HuR expression, whose 3'-UTR may promote colon carcinogenesis and serve as a novel potential biomarker for CRC therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Su C, Li D, Li N, Du Y, Yang C, Bai Y, Lin C, Li X and Zhang Y: Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer. Int J Oncol 52: 1479-1490, 2018.
APA
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y. ... Zhang, Y. (2018). Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer. International Journal of Oncology, 52, 1479-1490. https://doi.org/10.3892/ijo.2018.4305
MLA
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y., Lin, C., Li, X., Zhang, Y."Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer". International Journal of Oncology 52.5 (2018): 1479-1490.
Chicago
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y., Lin, C., Li, X., Zhang, Y."Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer". International Journal of Oncology 52, no. 5 (2018): 1479-1490. https://doi.org/10.3892/ijo.2018.4305
Copy and paste a formatted citation
x
Spandidos Publications style
Su C, Li D, Li N, Du Y, Yang C, Bai Y, Lin C, Li X and Zhang Y: Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer. Int J Oncol 52: 1479-1490, 2018.
APA
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y. ... Zhang, Y. (2018). Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer. International Journal of Oncology, 52, 1479-1490. https://doi.org/10.3892/ijo.2018.4305
MLA
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y., Lin, C., Li, X., Zhang, Y."Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer". International Journal of Oncology 52.5 (2018): 1479-1490.
Chicago
Su, C., Li, D., Li, N., Du, Y., Yang, C., Bai, Y., Lin, C., Li, X., Zhang, Y."Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer". International Journal of Oncology 52, no. 5 (2018): 1479-1490. https://doi.org/10.3892/ijo.2018.4305
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