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International Journal of Oncology
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Article

PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation

  • Authors:
    • Shijun Yu
    • Li Li
    • Qiong Wu
    • Ning Dou
    • Yandong Li
    • Yong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
  • Pages: 2011-2020
    |
    Published online on: March 20, 2018
       https://doi.org/10.3892/ijo.2018.4329
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Abstract

Protein phosphatase 2A (PP2A) is an essential serine/threonine protein phosphatase that regulates the basic activities of eukaryotes by dephosphorylating its substrates. The function and substrate specificity of PP2A are generally determined by its regulatory subunits. In the present study, the clinical significance and roles of PPP2R2D, one of the regulatory subunits of PP2A, were demonstrated in gastric cancer (GC) carcinogenesis. Through a tissue microarray and quantitative polymerase chain reaction analysis, it was demonstrated that PPP2R2D was commonly upregulated in GC samples. This upregulation was positively correlated with the patients' tumor stage (P<0.01), T classification (P<0.01) and N classification (P=0.01). Furthermore, a high expression of PPP2R2D was closely associated with poor prognosis of patients. Knockdown of PPP2R2D significantly inhibited the proliferation and migration of GC cells in vitro, as well as the tumorigenicity and metastasis in vivo in an animal GC model. By contrast, overexpression of PPP2R2D promoted GC cell proliferation and migration in vitro. The analysis of underlying mechanisms indicated that PPP2R2D silencing decreased the phosphorylation level of mechanistic target of rapamycin (mTOR), thereby implicating that PPP2R2D is involved in the regulation of mTOR activity during tumorigenesis. Thus, the findings of the present study suggested that PPP2R2D may serve as a potential oncogene in GC and as a novel target for therapeutic strategies against this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Yu S, Li L, Wu Q, Dou N, Li Y and Gao Y: PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol 52: 2011-2020, 2018.
APA
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., & Gao, Y. (2018). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. International Journal of Oncology, 52, 2011-2020. https://doi.org/10.3892/ijo.2018.4329
MLA
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., Gao, Y."PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation". International Journal of Oncology 52.6 (2018): 2011-2020.
Chicago
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., Gao, Y."PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation". International Journal of Oncology 52, no. 6 (2018): 2011-2020. https://doi.org/10.3892/ijo.2018.4329
Copy and paste a formatted citation
x
Spandidos Publications style
Yu S, Li L, Wu Q, Dou N, Li Y and Gao Y: PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol 52: 2011-2020, 2018.
APA
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., & Gao, Y. (2018). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. International Journal of Oncology, 52, 2011-2020. https://doi.org/10.3892/ijo.2018.4329
MLA
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., Gao, Y."PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation". International Journal of Oncology 52.6 (2018): 2011-2020.
Chicago
Yu, S., Li, L., Wu, Q., Dou, N., Li, Y., Gao, Y."PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation". International Journal of Oncology 52, no. 6 (2018): 2011-2020. https://doi.org/10.3892/ijo.2018.4329
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