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Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma

  • Authors:
    • Dawei Zhang
    • Haiyan Li
    • Juping Xie
    • Decan Jiang
    • Liangqi Cao
    • Xuewei Yang
    • Ping Xue
    • Xiaofeng Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China, Department of Breast and Thyroid Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510655, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1777-1786
    |
    Published online on: April 5, 2018
       https://doi.org/10.3892/ijo.2018.4362
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Abstract

The aim of the present study was to elucidate whether, and how, long intergenic non-protein coding RNA 1296 (LINC01296) is involved in the modulation of human cholangiocarcinoma (CCA) development and progression. Microarray data analysis and reverse transcription-quantitative polymerase chain reaction analysis demonstrated that LINC01296 was significantly upregulated in human CCA compared with nontumor tissues. Furthermore, the expression of LINC01296 in human CCA was positively associated with tumor severity and clinical stage. Knockdown of LINC01296 dramatically suppressed the viability, migration and invasion of RBE and CCLP1 cells, and promoted cell apoptosis in vitro. Furthermore, LINC01296 knockdown inhibited tumor growth in a xenograft model. Mechanistically, LINC01296 was demonstrated to sponge microRNA-5095 (miR-5095), which targets MYCN proto-oncogene bHLH transcription factor (MYCN) mRNA in human CCA. By inhibition of miR-5095, LINC01296 overexpression upregulated the expression of MYCN and promoted cell viability, migration and invasion in CCA cells. The results reveal that the axis of LINC01296/miR-5095/MYCN may be a mechanism to regulate CCA development and progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang D, Li H, Xie J, Jiang D, Cao L, Yang X, Xue P and Jiang X: Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma. Int J Oncol 52: 1777-1786, 2018.
APA
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X. ... Jiang, X. (2018). Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma. International Journal of Oncology, 52, 1777-1786. https://doi.org/10.3892/ijo.2018.4362
MLA
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X., Xue, P., Jiang, X."Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma". International Journal of Oncology 52.6 (2018): 1777-1786.
Chicago
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X., Xue, P., Jiang, X."Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma". International Journal of Oncology 52, no. 6 (2018): 1777-1786. https://doi.org/10.3892/ijo.2018.4362
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Li H, Xie J, Jiang D, Cao L, Yang X, Xue P and Jiang X: Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma. Int J Oncol 52: 1777-1786, 2018.
APA
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X. ... Jiang, X. (2018). Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma. International Journal of Oncology, 52, 1777-1786. https://doi.org/10.3892/ijo.2018.4362
MLA
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X., Xue, P., Jiang, X."Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma". International Journal of Oncology 52.6 (2018): 1777-1786.
Chicago
Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X., Xue, P., Jiang, X."Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma". International Journal of Oncology 52, no. 6 (2018): 1777-1786. https://doi.org/10.3892/ijo.2018.4362
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