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Article

Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1

  • Authors:
    • Myoung Jun Kim
    • Sung Kyung Choi
    • Seong Hwi Hong
    • Jung Woo Eun
    • Suk Woo Nam
    • Jeung-Whan Han
    • Jueng Soo You
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Research Institute of Medical Science, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea, Functional RNomics Research Center, College of Medicine, The Catholic University, Seoul 06591, Republic of Korea, Research Center for Epigenome Regulation, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea
  • Pages: 395-403
    |
    Published online on: May 2, 2018
       https://doi.org/10.3892/ijo.2018.4392
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Abstract

The interleukin-7 receptor (IL7R) is generally expressed in immune cells and is critical in survival, development and homeostasis in the immune system. Advanced genome-wide cancer studies have reported that IL7R is genetically amplified in human esophageal squamous cell carcinoma (ESCC), however, the exact role of IL7R in ESCC has not been investigated. In the present study, it was found that IL7R was overexpressed in ESCC cohorts and the loss of IL7R induced anti-oncogenic effects in ESCC cell lines. A small panel of epigenetic drugs were screened for their ability to downregulate the expression of IL7R. Unexpectedly, apicidin, a histone deacetylase (HDAC) inhibitor, effectively downregulated the expression of IL7R in a dose-dependent manner at an early time-point, as determined by quantitative polymerase chain reaction and IL7R immunostaining, and did not require de novo protein synthesis. Of note, apicidin induced the acetylation of Forkhead box-containing protein, O subfamily 1, which acts as a repressor at the IL7R promoter, accompanied with depleted active histone modifications based on chromatin immunoprecipitation assay. Taken together, these results demonstrated that targeting oncogenic IL7R in ESCC by HDAC inhibitors may be a valuable therapeutic approach.
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Copy and paste a formatted citation
Spandidos Publications style
Kim M, Choi S, Hong S, Eun J, Nam S, Han J and You J: Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1. Int J Oncol 53: 395-403, 2018.
APA
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., & You, J. (2018). Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1. International Journal of Oncology, 53, 395-403. https://doi.org/10.3892/ijo.2018.4392
MLA
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., You, J."Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1". International Journal of Oncology 53.1 (2018): 395-403.
Chicago
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., You, J."Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1". International Journal of Oncology 53, no. 1 (2018): 395-403. https://doi.org/10.3892/ijo.2018.4392
Copy and paste a formatted citation
x
Spandidos Publications style
Kim M, Choi S, Hong S, Eun J, Nam S, Han J and You J: Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1. Int J Oncol 53: 395-403, 2018.
APA
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., & You, J. (2018). Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1. International Journal of Oncology, 53, 395-403. https://doi.org/10.3892/ijo.2018.4392
MLA
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., You, J."Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1". International Journal of Oncology 53.1 (2018): 395-403.
Chicago
Kim, M., Choi, S., Hong, S., Eun, J., Nam, S., Han, J., You, J."Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1". International Journal of Oncology 53, no. 1 (2018): 395-403. https://doi.org/10.3892/ijo.2018.4392
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