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Article

Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene

  • Authors:
    • Chun‑Chi Chang
    • Ting-Yuan Liu
    • Ya‑Ting Lee
    • Yu-Chia Chen
    • Kun‑Tu Yeh
    • Chien‑Chin Lee
    • Ya‑Ling Chen
    • Pei-Chin Lin
    • Ya‑Sian Chang
    • Wen‑Ling Chan
    • Ta‑Chih Liu
    • Jan‑Gowth Chang
  • View Affiliations / Copyright

    Affiliations: Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, R.O.C., Center for Precision Medicine, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Epigenome Research Center, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan, R.O.C., Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500, Taiwan, R.O.C., Department of Laboratory Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
  • Pages: 417-433
    |
    Published online on: May 8, 2018
       https://doi.org/10.3892/ijo.2018.4398
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Abstract

Long non‑coding RNAs (lncRNAs) have various functions, including chromatin remodeling and the regulation of gene expression at the transcriptional and post-transcriptional levels. However, few lncRNAs have been investigated comprehensively, with the majority being uncharacterized. In the present study, a bioinformatics pipeline was established to identify novel lncRNA sequences similar to the 3'-untranslated regions (3'‑UTRs) of protein-coding genes. These pairs of lncRNAs and coding genes contained the same microRNA (miRNA) target sites; the lncRNA CR933609 matched the 3'‑UTR of INO80 complex subunit D (INO80D) mRNA. The expression levels of CR933609 and INO80D were significantly decreased in non‑small cell lung cancer (NSCLC) and other cancer tissues. The expression levels of CR933609 and INO80D were decreased in CR933609-knockdown NSCLC cells, but only expression levels of INO80D decreased in INO80D knockdown cells. It was shown that there are independent promoters in CR933609 and INO80D. It was also found that the expression levels of INO80D were downregulated by endogenous miRNA‑5096 in A549 cells, but not in CR933609-overexpressing A549 cells. Furthermore, the lncRNA CR933609 acted as a decoy to protect INO80D from downregulation by miRNA‑5096 in NSCLC cells. A protocol was established to identify novel lncRNAs in the 3'‑UTR and the existence of novel lncRNAs was confirmed.
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Copy and paste a formatted citation
Spandidos Publications style
Chang CC, Liu T, Lee YT, Chen Y, Yeh KT, Lee CC, Chen YL, Lin P, Chang YS, Chan WL, Chan WL, et al: Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene. Int J Oncol 53: 417-433, 2018.
APA
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C. ... Chang, J. (2018). Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene. International Journal of Oncology, 53, 417-433. https://doi.org/10.3892/ijo.2018.4398
MLA
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C., Chen, Y., Lin, P., Chang, Y., Chan, W., Liu, T., Chang, J."Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene". International Journal of Oncology 53.1 (2018): 417-433.
Chicago
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C., Chen, Y., Lin, P., Chang, Y., Chan, W., Liu, T., Chang, J."Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene". International Journal of Oncology 53, no. 1 (2018): 417-433. https://doi.org/10.3892/ijo.2018.4398
Copy and paste a formatted citation
x
Spandidos Publications style
Chang CC, Liu T, Lee YT, Chen Y, Yeh KT, Lee CC, Chen YL, Lin P, Chang YS, Chan WL, Chan WL, et al: Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene. Int J Oncol 53: 417-433, 2018.
APA
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C. ... Chang, J. (2018). Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene. International Journal of Oncology, 53, 417-433. https://doi.org/10.3892/ijo.2018.4398
MLA
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C., Chen, Y., Lin, P., Chang, Y., Chan, W., Liu, T., Chang, J."Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene". International Journal of Oncology 53.1 (2018): 417-433.
Chicago
Chang, C., Liu, T., Lee, Y., Chen, Y., Yeh, K., Lee, C., Chen, Y., Lin, P., Chang, Y., Chan, W., Liu, T., Chang, J."Genome-wide analysis of lncRNAs in 3'-untranslated regions: CR933609 acts as a decoy to protect the INO80D gene". International Journal of Oncology 53, no. 1 (2018): 417-433. https://doi.org/10.3892/ijo.2018.4398
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