Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells

  • Authors:
    • Minghua Liu
    • Ge Zhao
    • Dan Zhang
    • Weixiao An
    • Honglin Lai
    • Xiaofang Li
    • Shousong Cao
    • Xiukun Lin
  • View Affiliations

  • Published online on: July 2, 2018     https://doi.org/10.3892/ijo.2018.4465
  • Pages: 1363-1373
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Abstract

Previous studies by our group have demonstrated that extract of clove exhibits potent anticancer effects in vitro and in vivo. In the present study, the effect of an extracted and isolated active fraction of clove (AFC) on induction of cellular apoptosis in human colorectal cancer HCT-116 cells was investigated by morphological observation, flow cytometry, and western blotting analysis. The results revealed that AFC induced apoptosis of HCT-116 cells. AFC also induced autophagy, demonstrated by increased punctuate microtubule-associated protein 1A/1B-light chain 3 (LC3) staining, and LC3-II and Beclin-1 protein expression levels. Furthermore, the autophagy inhibitors 3-MA and baflomycin A1 potentiated the pro-apoptotic activity of AFC in HCT-116 cells. AFC also inhibited the phosphorylation of the phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin signaling pathway. The present study may improve the existing understanding of the anticancer mechanisms of clove and provide a scientific rationale for AFC to be further developed as a promising novel anticancer agent for the treatment of colorectal cancer.
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September-2018
Volume 53 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu M, Zhao G, Zhang D, An W, Lai H, Li X, Cao S and Lin X: Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells. Int J Oncol 53: 1363-1373, 2018
APA
Liu, M., Zhao, G., Zhang, D., An, W., Lai, H., Li, X. ... Lin, X. (2018). Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells. International Journal of Oncology, 53, 1363-1373. https://doi.org/10.3892/ijo.2018.4465
MLA
Liu, M., Zhao, G., Zhang, D., An, W., Lai, H., Li, X., Cao, S., Lin, X."Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells". International Journal of Oncology 53.3 (2018): 1363-1373.
Chicago
Liu, M., Zhao, G., Zhang, D., An, W., Lai, H., Li, X., Cao, S., Lin, X."Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells". International Journal of Oncology 53, no. 3 (2018): 1363-1373. https://doi.org/10.3892/ijo.2018.4465