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Article

Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT

  • Authors:
    • Rong Li
    • Changli Wu
    • Hongying Liang
    • Yinghai Zhao
    • Chunyan Lin
    • Xiujuan Zhang
    • Caiguo Ye
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Pathophysiology, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China, Department of Physiology, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China, Laboratory of Physiological Science, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China, China-America United Cancer Research Institute, Guangdong Medical University, Dongguan, Guangdong 523800, P.R. China
  • Pages: 1763-1773
    |
    Published online on: July 20, 2018
       https://doi.org/10.3892/ijo.2018.4495
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Abstract

The transcription factor twist family bHLH transcription factor 1 (TWIST), which is a member of the basic helix-loop-helix class of proteins, is known to induce epithelial-mesenchymal transition (EMT) and promote cancer metastasis. TWIST has previously been reported to be associated with multidrug resistance (MDR), since its depletion increases drug sensitivity. Although these previous studies have established a strong association between EMT and MDR, the molecular mechanism remains obscure. The present study demonstrated that TWIST protein expression was elevated in liver cancer, and was positively correlated with multidrug resistance protein 1 (MDR1) expression. Conversely, MDR1 was negatively correlated with E‑cadherin expression in liver cancer samples. In addition, the present study indicated that doxorubicin-resistant HepG2 (R‑HepG2) cells acquired an EMT phenotype. TWIST was also more highly expressed in R‑HepG2 cells compared with in parental HepG2 cells. Knockdown of TWIST increased the sensitivity of R‑HepG2 cells to 5-fluroracil, cisplatin and doxorubicin through a reduction in MDR1 expression and drug efflux ability. Furthermore, knockdown of TWIST in R‑HepG2 cells inhibited the migratory ability of cells and suppressed the EMT phenotype. These findings demonstrated that targeting TWIST may be considered a novel strategy to overcome drug resistance in liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li R, Wu C, Liang H, Zhao Y, Lin C, Zhang X and Ye C: Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT. Int J Oncol 53: 1763-1773, 2018.
APA
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., & Ye, C. (2018). Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT. International Journal of Oncology, 53, 1763-1773. https://doi.org/10.3892/ijo.2018.4495
MLA
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., Ye, C."Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT". International Journal of Oncology 53.4 (2018): 1763-1773.
Chicago
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., Ye, C."Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT". International Journal of Oncology 53, no. 4 (2018): 1763-1773. https://doi.org/10.3892/ijo.2018.4495
Copy and paste a formatted citation
x
Spandidos Publications style
Li R, Wu C, Liang H, Zhao Y, Lin C, Zhang X and Ye C: Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT. Int J Oncol 53: 1763-1773, 2018.
APA
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., & Ye, C. (2018). Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT. International Journal of Oncology, 53, 1763-1773. https://doi.org/10.3892/ijo.2018.4495
MLA
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., Ye, C."Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT". International Journal of Oncology 53.4 (2018): 1763-1773.
Chicago
Li, R., Wu, C., Liang, H., Zhao, Y., Lin, C., Zhang, X., Ye, C."Knockdown of TWIST enhances the cytotoxicity of chemotherapeutic drugs in doxorubicin-resistant HepG2 cells by suppressing MDR1 and EMT". International Journal of Oncology 53, no. 4 (2018): 1763-1773. https://doi.org/10.3892/ijo.2018.4495
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