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Article

Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions

  • Authors:
    • Li Zhou
    • Mu-Lu Zhan
    • Yi Tang
    • Ming Xiao
    • Ming Li
    • Qing-Shu Li
    • Lian Yang
    • Xian Li
    • Wen-Wen Chen
    • Ya-Lan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 1613-1624
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/ijo.2018.4506
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Abstract

Type 2 diabetes mellitus (T2DM) is associated with an increased risk of the development of colorectal cancer (CRC). A previous study revealed that the levels of arginine-specific mono-ADP-ribosyltransferase 1 (ART1) in CRC tissues from patients with T2DM were higher than in non-diabetic patients. Hyperglycemia, which is a risk factor of cancer, is a common feature of T2DM; however, the effects of ART1 on glycolysis and energy metabolism in CRC cells under high-glucose conditions remains to be elucidated. β-caryophyllene (BCP) has been reported to exert anticancer and hypoglycemic effects. In the present study, CT26 cells were cultured under a high-glucose conditions and the expression levels of relevant factors were detected by western blotting. Cell Counting Kit-8 assay, flow cytometry, Hoechst 33258 staining, ATP assay and lactic acid assay were used to detect the proliferation, apoptosis and energy metabolism of CT26 cells. To observe the effects of ART1 and BCP on tumor growth in vivo, CT26 cell tumors were successfully transplanted into BALB/c mice with T2DM. The results demonstrated that overexpression of ART1 may increase glycolysis and energy metabolism in CT26 CRC cells under high glucose conditions by regulating the protein kinase B/mammalian target of rapamycin/c‑Myc signaling pathway and the expression of glycolytic enzymes. BCP inhibited the effects induced by ART1, which may be due to a BCP-induced reduction in the expression levels of ART1 via nuclear factor-κB. Therefore, ART1 may be considered a therapeutic target for the treatment of diabetic patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, Zhan M, Tang Y, Xiao M, Li M, Li Q, Yang L, Li X, Chen W, Wang Y, Wang Y, et al: Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions. Int J Oncol 53: 1613-1624, 2018.
APA
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q. ... Wang, Y. (2018). Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions. International Journal of Oncology, 53, 1613-1624. https://doi.org/10.3892/ijo.2018.4506
MLA
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q., Yang, L., Li, X., Chen, W., Wang, Y."Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions". International Journal of Oncology 53.4 (2018): 1613-1624.
Chicago
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q., Yang, L., Li, X., Chen, W., Wang, Y."Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions". International Journal of Oncology 53, no. 4 (2018): 1613-1624. https://doi.org/10.3892/ijo.2018.4506
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Zhan M, Tang Y, Xiao M, Li M, Li Q, Yang L, Li X, Chen W, Wang Y, Wang Y, et al: Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions. Int J Oncol 53: 1613-1624, 2018.
APA
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q. ... Wang, Y. (2018). Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions. International Journal of Oncology, 53, 1613-1624. https://doi.org/10.3892/ijo.2018.4506
MLA
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q., Yang, L., Li, X., Chen, W., Wang, Y."Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions". International Journal of Oncology 53.4 (2018): 1613-1624.
Chicago
Zhou, L., Zhan, M., Tang, Y., Xiao, M., Li, M., Li, Q., Yang, L., Li, X., Chen, W., Wang, Y."Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions". International Journal of Oncology 53, no. 4 (2018): 1613-1624. https://doi.org/10.3892/ijo.2018.4506
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