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Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer

  • Authors:
    • Guangye Han
    • Xinjun Zhang
    • Pei Liu
    • Quanfeng Yu
    • Zeyu Li
    • Qinnan Yu
    • Xiaoxia Wei
  • View Affiliations / Copyright

    Affiliations: The Second Ward of Urology Department, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China, The First Ward of Urology Department, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China, The Second Ward of Infection Department, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2056-2066
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    Published online on: August 16, 2018
       https://doi.org/10.3892/ijo.2018.4526
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Abstract

Prostate cancer (PCa) is one of the most common malignancies among males worldwide. Anti-silencing function 1B histone chaperone (ASF1B) has been reported to be involved in PCa. The present study aimed to investigate the role and molecular mechanism of ASF1B in PCa. Data of genes were obtained from The Cancer Genome Atlas database. The core gene was identified using the DAVID website. Cell viability and colony formation were detected using a cell counting kit-8 assay and crystal violet staining, respectively. Cell cycle distribution and apoptosis were assessed using flow cytometry analysis. The corresponding factors were analyzed by reverse transcription-quantitative polymerase chain reaction and western blotting. It was demonstrated that ASF1B was highly expressed in the PCa tissues and cells compared with the non-PCa tissues and cells, respectively. While siRNA-ASF1B significantly reduced the viability and colony formation, it promoted apoptosis, G1 phase cell cycle arrest of LNCap as well as C4-2 cells. siRNA-ASF1B was revealed to significantly reduce the level of B-cell lymphoma-2 and cyclin D1, and enhance the expression levels of p53, caspase-3 and Bcl-2 associated X protein. Furthermore, the phosphorylation levels of phosphatidylinositol 3 kinase (PI3K) and protein kinase B (Akt) were significantly decreased in the siRNA-ASF1B group compared with the mock group. In summary, the present study demonstrated that silencing of ASF1B suppressed the proliferation, and promoted apoptosis and cell cycle arrest of PCa cells. Inhibition of the PI3K/Akt signaling pathway was pertinent to the role of si-ASF1B. This phenomenon suggests that the downregulation of ASF1B may aid in inhibiting the progression of PCa.
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Copy and paste a formatted citation
Spandidos Publications style
Han G, Zhang X, Liu P, Yu Q, Li Z, Yu Q and Wei X: Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer. Int J Oncol 53: 2056-2066, 2018.
APA
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., & Wei, X. (2018). Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer. International Journal of Oncology, 53, 2056-2066. https://doi.org/10.3892/ijo.2018.4526
MLA
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., Wei, X."Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer". International Journal of Oncology 53.5 (2018): 2056-2066.
Chicago
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., Wei, X."Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer". International Journal of Oncology 53, no. 5 (2018): 2056-2066. https://doi.org/10.3892/ijo.2018.4526
Copy and paste a formatted citation
x
Spandidos Publications style
Han G, Zhang X, Liu P, Yu Q, Li Z, Yu Q and Wei X: Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer. Int J Oncol 53: 2056-2066, 2018.
APA
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., & Wei, X. (2018). Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer. International Journal of Oncology, 53, 2056-2066. https://doi.org/10.3892/ijo.2018.4526
MLA
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., Wei, X."Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer". International Journal of Oncology 53.5 (2018): 2056-2066.
Chicago
Han, G., Zhang, X., Liu, P., Yu, Q., Li, Z., Yu, Q., Wei, X."Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer". International Journal of Oncology 53, no. 5 (2018): 2056-2066. https://doi.org/10.3892/ijo.2018.4526
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