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Article

MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo

  • Authors:
    • Jiaqi Cui
    • Chunmei Wei
    • Linli Deng
    • Xingyi Kuang
    • Zengtie Zhang
    • Chryso Pierides
    • Jianxiang Chi
    • Li Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Pathology, Xi'an Jiao Tong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China, Center for the Study of Haematological Malignancies, Karaiskakio Foundation, 2032 Nicosia, Cyprus
  • Pages: 2191-2199
    |
    Published online on: August 22, 2018
       https://doi.org/10.3892/ijo.2018.4534
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Abstract

Whilst the role of microRNA‑143 (miR‑143) in myelodysplastic syndrome (MDS) remains unclear, abnormally expressed microRNA‑143 has been detected in many types of cancer tissues. In this study, we describe a cohort study for the verification of miR‑143 expression, as well as the investigation of the molecular mechanisms of miR‑143 in MDS/acute myeloid leukaemia (AML). In a series of experiments, miR‑143 recombinant lentiviral vectors transformed into SKM‑1 cells were either overexpressed or knocked down, and the results illustrated that the overexpression of miR‑143 inhibited SKM‑1 cell growth, arrested the SKM‑1 cells in the G0/G1 phase, interfered with cell proliferation and induced cell apoptosis via the Fas/FasL pathway. Conversely, miR‑143 knockdown induced a decrease in the apoptosis and promoted the proliferation of SKM‑1 cells. Moreover, miR‑143 was shown to suppress MLLT3/AF9 expression by binding to its 3'‑UTR. Taken together, the findings of this study indicate that miR‑143 may be a critical regulator of MDS/AML cell carcinogenesis, acting as a potent antitumour molecular target for the diagnosis or treatment of cancers associated with the abnormal expression of MLLT3/AF9, hence facilitating the development of potential therapeutics against MDS/AML.
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Copy and paste a formatted citation
Spandidos Publications style
Cui J, Wei C, Deng L, Kuang X, Zhang Z, Pierides C, Chi J and Wang L: MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo. Int J Oncol 53: 2191-2199, 2018.
APA
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C. ... Wang, L. (2018). MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo. International Journal of Oncology, 53, 2191-2199. https://doi.org/10.3892/ijo.2018.4534
MLA
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C., Chi, J., Wang, L."MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo". International Journal of Oncology 53.5 (2018): 2191-2199.
Chicago
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C., Chi, J., Wang, L."MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo". International Journal of Oncology 53, no. 5 (2018): 2191-2199. https://doi.org/10.3892/ijo.2018.4534
Copy and paste a formatted citation
x
Spandidos Publications style
Cui J, Wei C, Deng L, Kuang X, Zhang Z, Pierides C, Chi J and Wang L: MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo. Int J Oncol 53: 2191-2199, 2018.
APA
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C. ... Wang, L. (2018). MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo. International Journal of Oncology, 53, 2191-2199. https://doi.org/10.3892/ijo.2018.4534
MLA
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C., Chi, J., Wang, L."MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo". International Journal of Oncology 53.5 (2018): 2191-2199.
Chicago
Cui, J., Wei, C., Deng, L., Kuang, X., Zhang, Z., Pierides, C., Chi, J., Wang, L."MicroRNA‑143 increases cell apoptosis in myelodysplastic syndrome through the Fas/FasL pathway both in vitro and in vivo". International Journal of Oncology 53, no. 5 (2018): 2191-2199. https://doi.org/10.3892/ijo.2018.4534
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