Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
December-2018 Volume 53 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2018 Volume 53 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation

  • Authors:
    • Hong Lu
    • Jie Chen
    • Yongming Luo
    • Huanjun Xu
    • Ling Xiong
    • Jianjiang Fu
  • View Affiliations / Copyright

    Affiliations: Network and Educational Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China, Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China
  • Pages: 2695-2704
    |
    Published online on: October 10, 2018
       https://doi.org/10.3892/ijo.2018.4592
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Curcolonol (CCL) is a furan type sesquiterpene isolated from several medical herbs. Based on previous results of anti-migratory activity screening, in this study, we investigated the effects of CCL on cancer cell motility. By in vitro migration assay, we found that CCL significantly inhibited the vertical and horizontal migration of breast cancer cells induced by transforming growth factor (TGF)-β1. In addition, CCL also exerted inhibitory effects on F-actin polymerization in breast cancer cells when the cells were dyed with phalloidin. Given the close association between F-actin and ADF/cofilin, the effects of CCL on the expression and phosphorylation of cofilin 1 were explored. It was observed that there were minimal changes in the expression of cofilin 1; however, the phosphorylation of cofilin 1 was significantly inhibited by CCL in a dose-dependent manner. Furthermore, CCL significantly inhibited the activity of LIM kinase 1 (LIMK1), although almost no effects were observed on LIMK1 expression and phosphorylation. However, the inhibitory effects of CCL on LIMK1 activity were antagonized and enhanced by the overexpression and knockdown of LIMK1, respectively. Based on the current data, it is thus suggested that the suppressive effects of CCL on breast cancer cell motility are due to its potential to reduce the phosphorylation of cofilin 1, which may be associated with the inhibition of the catalytic activity of LIMK1.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Vanharanta S and Massagué J: Origins of metastatic traits. Cancer Cell. 24:410–421. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Chiang AC and Massagué J: Molecular basis of metastasis. N Engl J Med. 359:2814–2823. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Binamé F, Pawlak G, Roux P and Hibner U: What makes cells move: Requirements and obstacles for spontaneous cell motility. Mol Biosyst. 6:648–661. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Carlier MF, Pernier J, Montaville P, Shekhar S, Kühn S and Cytoskeleton Dynamics; Motility group: Control of polarized assembly of actin filaments in cell motility. Cell Mol Life Sci. 72:3051–3067. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Shekhar S, Pernier J and Carlier MF: Regulators of actin filament barbed ends at a glance. J Cell Sci. 129:1085–1091. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Kanellos G and Frame MC: Cellular functions of the ADF/cofilin family at a glance. J Cell Sci. 129:3211–3218. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Ostrowska Z and Moraczewska J: Cofilin - a protein controlling dynamics of actin filaments. Postepy Hig Med Dosw. 71:339–351. 2017. View Article : Google Scholar

8 

He L, Seitz SP, Trainor GL, Tortolani D, Vaccaro W, Poss M, Tarby CM, Tokarski JS, Penhallow B, Hung CY, et al: Modulation of cofilin phosphorylation by inhibition of the Lim family kinases. Bioorg Med Chem Lett. 22:5995–5998. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Prunier C, Prudent R, Kapur R, Sadoul K and Lafanechère L: LIM kinases: Cofilin and beyond. Oncotarget. 8:41749–41763. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Bernard O: Lim kinases, regulators of actin dynamics. Int J Biochem Cell Biol. 39:1071–1076. 2007. View Article : Google Scholar

11 

Scott RW and Olson MF: LIM kinases: Function, regulation and association with human disease. J Mol Med (Berl). 85:555–568. 2007. View Article : Google Scholar

12 

Park GB and Kim D: PI3K catalytic isoform alteration promotes the LIMK1-related metastasis through the PAK1 or ROCK1/2 activation in cigarette smoke-exposed ovarian cancer cells. Anticancer Res. 37:1805–1818. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Wan L, Zhang L, Fan K and Wang J: MiR-27b targets LIMK1 to inhibit growth and invasion of NSCLC cells. Mol Cell Biochem. 390:85–91. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Liao Q, Li R, Zhou R, Pan Z, Xu L, Ding Y and Zhao L: LIM kinase 1 interacts with myosin-9 and alpha-actinin-4 and promotes colorectal cancer progression. Br J Cancer. 117:563–571. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Liu YH, Wu PQ, Hu QL, Pei YJ, Qi FM, Zhang ZX and Fei DQ: Cytotoxic and antibacterial activities of iridoids and sesquiterpenoids from Valeriana jatamansi. Fitoterapia. 123:73–78. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Editorial Committee of the Administration Bureau of Traditional Chinese Medicine: Genus Chloranthaceae. Chinese Materia Medica (Zhonghua Bencao). Shanghai Science & Technology Press; Shanghai: pp. 2051–2061. 1998

17 

Fu J, Ke X, Tan S, Liu T, Wang S, Ma J and Lu H: The natural compound codonolactone attenuates TGF-β1-mediated epithelial-to-mesenchymal transition and motility of breast cancer cells. Oncol Rep. 35:117–126. 2016. View Article : Google Scholar

18 

Wang W, Chen B, Zou R, Tu X, Tan S, Lu H, Liu Z and Fu J: Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2. Int J Oncol. 45:1891–1900. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Fu J, Wang S, Lu H, Ma J, Ke X, Liu T and Luo Y: In vitro inhibitory effects of terpenoids from Chloranthus multistachys on epithelial-mesenchymal transition via down-regulation of Runx2 activation in human breast cancer. Phytomedicine. 22:165–172. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Zhang SS, Fu JJ, Chen HY, Tu LF, Xiao CR, Zhang RZ, Liu DP and Luo YM: Sesquiterpenes with anti-metastasis breast cancer activity fro Chloranthus henryi. Zhongguo Zhong Yao Za Zhi. 42:3938–3944. 2017.In Chinese. PubMed/NCBI

21 

Iwasa JH and Mullins RD: Spatial and temporal relationships between actin-filament nucleation, capping, and disassembly. Curr Biol. 17:395–406. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Meberg PJ: Signal-regulated ADF/cofilin activity and growth cone motility. Mol Neurobiol. 21:97–107. 2000. View Article : Google Scholar

23 

Mizuno K: Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation. Cell Signal. 25:457–469. 2013. View Article : Google Scholar

24 

Shishkin S, Eremina L, Pashintseva N, Kovalev L and Kovaleva M: Cofilin-1 and other ADF/cofilin superfamily members in human malignant cells. Int J Mol Sci. 18:E102016. View Article : Google Scholar : PubMed/NCBI

25 

Wang W, Eddy R and Condeelis J: The cofilin pathway in breast cancer invasion and metastasis. Nat Rev Cancer. 7:429–440. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Lee YJ, Mazzatti DJ, Yun Z and Keng PC: Inhibition of invasiveness of human lung cancer cell line H1299 by overexpression of cofilin. Cell Biol Int. 29:877–883. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Yap CT, Simpson TI, Pratt T, Price DJ and Maciver SK: The motility of glioblastoma tumour cells is modulated by intracellular cofilin expression in a concentration-dependent manner. Cell Motil Cytoskeleton. 60:153–165. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Hotulainen P, Paunola E, Vartiainen MK and Lappalainen P: Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. Mol Biol Cell. 16:649–664. 2005. View Article : Google Scholar :

29 

Manetti F: LIM kinases are attractive targets with many macromolecular partners and only a few small molecule regulators. Med Res Rev. 32:968–998. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Su B, Su J, Zeng Y, Liu F, Xia H, Ma YH, Zhou ZG, Zhang S, Yang BM, Wu YH, et al: Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget. 7:10498–10512. 2016.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lu H, Chen J, Luo Y, Xu H, Xiong L and Fu J: Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. Int J Oncol 53: 2695-2704, 2018.
APA
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., & Fu, J. (2018). Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. International Journal of Oncology, 53, 2695-2704. https://doi.org/10.3892/ijo.2018.4592
MLA
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., Fu, J."Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation". International Journal of Oncology 53.6 (2018): 2695-2704.
Chicago
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., Fu, J."Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation". International Journal of Oncology 53, no. 6 (2018): 2695-2704. https://doi.org/10.3892/ijo.2018.4592
Copy and paste a formatted citation
x
Spandidos Publications style
Lu H, Chen J, Luo Y, Xu H, Xiong L and Fu J: Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. Int J Oncol 53: 2695-2704, 2018.
APA
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., & Fu, J. (2018). Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. International Journal of Oncology, 53, 2695-2704. https://doi.org/10.3892/ijo.2018.4592
MLA
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., Fu, J."Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation". International Journal of Oncology 53.6 (2018): 2695-2704.
Chicago
Lu, H., Chen, J., Luo, Y., Xu, H., Xiong, L., Fu, J."Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation". International Journal of Oncology 53, no. 6 (2018): 2695-2704. https://doi.org/10.3892/ijo.2018.4592
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team