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Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer

  • Authors:
    • Yue Song
    • Rui Wang
    • Li-Wei Li
    • Xi Liu
    • Yun-Fei Wang
    • Qi-Xue Wang
    • Qingyu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Phase I Clinical Trial, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300000, P.R. China, Department of Gastroenterology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Gastroenterology, Tianjin Nankai Hospital, Tianjin 300000, P.R. China, Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 77-86
    |
    Published online on: November 5, 2018
       https://doi.org/10.3892/ijo.2018.4625
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Abstract

HOX transcript antisense intergenic RNA (HOTAIR), a well‑known long non‑coding RNA, plays an important role in the regulation of epithelial‑to‑mesenchymal transition (EMT). In this study, we propose a novel mechanism through which HOTAIR promotes EMT by switching histone H3 lysine 27 acetylation to methylation at the E‑cadherin promoter, which induces the transcriptional inhibition of E‑cadherin. HOTAIR recruits polycomb repressive complex 2 (PRC2) to catalyze H3K27me3; however, whether HOTAIR is associated with the acetylation of histone H3 lysine 27, a marker of transcriptional activation, and the mechanisms through which HOTAIR triggers the metastasis of gastric cancer (GC) by epigenetic regulation remain largely unknown. In this study, HOTAIR knockdown significantly reversed EMT by increasing the expression of E‑cadherin in GC cells. Additionally, the loss of PRC2 activity induced by HOTAIR knockdown resulted in a global decrease in H3K27 methylation and an increase in H3K27 acetylation. Furthermore, HOTAIR recruits PRC2 (which consists of H3K27 methyltransferase EZH2, SUZ12 and EED), which may inhibit the reaction between the acetyltransferase CBP and H3K27 acetylation. On the whole, the findings of this study suggested that the HOTAIR‑mediated acetylation to methylation switch was associated with the transcriptional inhibition of E‑cadherin. HOTAIR can promote the development of GC through the epigenetic regulation of E‑cadherin, switching the state of the E‑cadherin promoter from the transcriptionally active to the transcriptionally repressive state.
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Copy and paste a formatted citation
Spandidos Publications style
Song Y, Wang R, Li L, Liu X, Wang Y, Wang Q and Zhang Q: Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer. Int J Oncol 54: 77-86, 2019.
APA
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., & Zhang, Q. (2019). Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer. International Journal of Oncology, 54, 77-86. https://doi.org/10.3892/ijo.2018.4625
MLA
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., Zhang, Q."Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer". International Journal of Oncology 54.1 (2019): 77-86.
Chicago
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., Zhang, Q."Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer". International Journal of Oncology 54, no. 1 (2019): 77-86. https://doi.org/10.3892/ijo.2018.4625
Copy and paste a formatted citation
x
Spandidos Publications style
Song Y, Wang R, Li L, Liu X, Wang Y, Wang Q and Zhang Q: Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer. Int J Oncol 54: 77-86, 2019.
APA
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., & Zhang, Q. (2019). Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer. International Journal of Oncology, 54, 77-86. https://doi.org/10.3892/ijo.2018.4625
MLA
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., Zhang, Q."Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer". International Journal of Oncology 54.1 (2019): 77-86.
Chicago
Song, Y., Wang, R., Li, L., Liu, X., Wang, Y., Wang, Q., Zhang, Q."Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer". International Journal of Oncology 54, no. 1 (2019): 77-86. https://doi.org/10.3892/ijo.2018.4625
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