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Article

EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process

  • Authors:
    • Xia Chen
    • Bin Lu
    • Qian Ma
    • Cheng‑Dong Ji
    • Jian‑Zhong Li
  • View Affiliations / Copyright

    Affiliations: Key Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China, International Joint Cancer Institute, Second Military Medical University, Shanghai 200433, P.R. China, Department of Scientific Research Management, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China, Department of Biochemical Pharmacy, Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 722-732
    |
    Published online on: November 21, 2018
       https://doi.org/10.3892/ijo.2018.4639
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Abstract

Eph receptor tyrosine kinases are critical for cell‑cell communication during normal and oncogenic development. Eph receptor A3 (EphA3) expression is associated with tumor promotion in certain types of cancer; however, it acts as a tumor suppressor in others. The expression levels of EphA3 and its effects on tumor progression in esophageal squamous cell carcinoma (ESCC) cell lines were determined using reverse transcription‑quantitative polymerase chain reaction analysis and a Transwell invasion assay. The present study demonstrated that EphA3 expression was decreased in ESCC tissues and cell lines. Treatment with the DNA methylation inhibitor 5‑aza‑2'‑deoxycytidine increased the mRNA expression levels of EphA3 in the ESCC cell lines KYSE510 and KYSE30. In addition, overexpression of EphA3 in KYSE450 and KYSE510 cells inhibited cell migration and invasion. EphA3 overexpression also decreased RhoA GTPase. Furthermore, EphA3 overexpression induced mesenchymal‑epithelial transition, as demonstrated by epithelial‑like morphological alterations, increased expression of epithelial proteins (E‑cadherin and the tight junction protein 1 zonula occludens‑1) and decreased expression of mesenchymal proteins (Vimentin, N‑cadherin and Snail). Conversely, silencing EphA3 in KYSE410 cells triggered epithelial‑mesenchymal transition, and promoted cell migration and invasion. These results suggested that EphA3 may serve a tumor‑suppressor role in ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Lu B, Ma Q, Ji CD and Li JZ: EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process. Int J Oncol 54: 722-732, 2019.
APA
Chen, X., Lu, B., Ma, Q., Ji, C., & Li, J. (2019). EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process. International Journal of Oncology, 54, 722-732. https://doi.org/10.3892/ijo.2018.4639
MLA
Chen, X., Lu, B., Ma, Q., Ji, C., Li, J."EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process". International Journal of Oncology 54.2 (2019): 722-732.
Chicago
Chen, X., Lu, B., Ma, Q., Ji, C., Li, J."EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process". International Journal of Oncology 54, no. 2 (2019): 722-732. https://doi.org/10.3892/ijo.2018.4639
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Lu B, Ma Q, Ji CD and Li JZ: EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process. Int J Oncol 54: 722-732, 2019.
APA
Chen, X., Lu, B., Ma, Q., Ji, C., & Li, J. (2019). EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process. International Journal of Oncology, 54, 722-732. https://doi.org/10.3892/ijo.2018.4639
MLA
Chen, X., Lu, B., Ma, Q., Ji, C., Li, J."EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process". International Journal of Oncology 54.2 (2019): 722-732.
Chicago
Chen, X., Lu, B., Ma, Q., Ji, C., Li, J."EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal‑epithelial transition process". International Journal of Oncology 54, no. 2 (2019): 722-732. https://doi.org/10.3892/ijo.2018.4639
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