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Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis

  • Authors:
    • Wei Zhang
    • Xiaoxuan Ou
    • Xiaohua Wu
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    Affiliations: Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1719-1733
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    Published online on: March 7, 2019
       https://doi.org/10.3892/ijo.2019.4742
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Abstract

Ovarian cancer remains the most lethal type of cancer among all gynecological malignancies. The majority of patients are diagnosed with ovarian cancer at the late stages of the disease. Therefore, there exists an imperative need for the development of early ovarian cancer diagnostic techniques. Exosomes, secreted by various cell types, play pivotal roles in intercellular communication, which emerge as promising diagnostic and prognostic biomarkers for ovarian cancer. In this study, we present for the first time, at least to the best of our knowledge, the proteomics profiling of exosomes derived from the plasma of patients with ovarian cancer via liquid chromatography tandem mass spectrometry (LC‑MS/MS) with tandem mass tagging (TMT). The exosomes enriched from patient plasma samples were characterized by nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), transmission electron microscopy (TEM) and western blot analysis. The size of the plasma exosomes fell into the range of 30 to 100 nm in diameter. The exosomal marker proteins, CD81 and TSG101, were clearly stained in the exosome samples; however, there was no staining for the endoplasmic reticulum protein, calnexin. A total of 294 proteins were identified with all exosome samples. Among these, 225 proteins were detected in both the cancerous and non‑cancerous samples. Apart from universal exosomal proteins, exosomes derived from ovarian cancer patient plasma also contained tumor‑specific proteins relevant to tumorigenesis and metastasis, particularly in epithelial ovarian carcinoma (EOC). Patients with EOC often suffer from coagulation dysfunction. The function of exosomes in coagulation was also examined. Several genes relevant to the coagulation cascade were screened out as promising diagnostic and prognostic factors that may play important roles in ovarian cancer progression and metastasis. On the whole, in this study, we successfully isolated and purified exosomes from plasma of patients with EOC, and identified a potential role of these exosomes in the coagulation cascade, as well as in the diagnosis and prognosis of patients.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Ou X and Wu X: Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. Int J Oncol 54: 1719-1733, 2019.
APA
Zhang, W., Ou, X., & Wu, X. (2019). Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. International Journal of Oncology, 54, 1719-1733. https://doi.org/10.3892/ijo.2019.4742
MLA
Zhang, W., Ou, X., Wu, X."Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis". International Journal of Oncology 54.5 (2019): 1719-1733.
Chicago
Zhang, W., Ou, X., Wu, X."Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis". International Journal of Oncology 54, no. 5 (2019): 1719-1733. https://doi.org/10.3892/ijo.2019.4742
Copy and paste a formatted citation
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Spandidos Publications style
Zhang W, Ou X and Wu X: Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. Int J Oncol 54: 1719-1733, 2019.
APA
Zhang, W., Ou, X., & Wu, X. (2019). Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. International Journal of Oncology, 54, 1719-1733. https://doi.org/10.3892/ijo.2019.4742
MLA
Zhang, W., Ou, X., Wu, X."Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis". International Journal of Oncology 54.5 (2019): 1719-1733.
Chicago
Zhang, W., Ou, X., Wu, X."Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis". International Journal of Oncology 54, no. 5 (2019): 1719-1733. https://doi.org/10.3892/ijo.2019.4742
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