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Article

Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer

  • Authors:
    • Wencai Guan
    • Xingxing Wang
    • Qunbo Lin
    • Jinguo Zhang
    • Weimin Ren
    • Guoxiong Xu
  • View Affiliations / Copyright

    Affiliations: Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China
  • Pages: 267-276
    |
    Published online on: May 28, 2019
       https://doi.org/10.3892/ijo.2019.4815
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Abstract

We previously reported that cystatin B (CSTB) is a progression marker of human ovarian cancer (OC); however, the regulatory mechanism of CSTB and its function in OC remain unclear. The present study aimed to explore the mechanism underlying transforming growth factor-β (TGF‑β) 1‑mediated CSTB regulation, and to examine the function of CSTB on OC cell proliferation and apoptosis. Using the online program, miRWalk, a microRNA (miR)‑143‑3p was detected, which contains a homologous sequence of the potential binding site to the 3'‑untranslated region (3'‑UTR) of CSTB. A dual‑luciferase reporter assay confirmed the interaction between miR‑143‑3p and CSTB 3'‑UTR. Treating OC cells with miR‑143‑3p mimics or inhibitors resulted in a decrease or an increase of CSTB expression at mRNA and protein levels, respectively. Additionally, CSTB was significantly overexpressed, whereas miR‑143‑3p was downregulated in human OC tissues compared with normal ovarian tissues. A negative correlation between miR‑143‑3p and CSTB mRNA expression was observed in ovarian malignant tumors. The levels of primary and mature miR‑143‑3p expression were upregulated in OC cells after TGF‑β1 treatment; the action of TGF‑β1 was abolished in the presence of an inhibitor of TGF‑β type I receptor. These results indicated an axis between TGF‑β, miR‑143‑3p and CSTB in OC cells. Furthermore, high levels of CSTB expression were associated with the poor overall survival of patients with OC. Knockdown of CSTB resulted in a decrease in OC cell proliferation and arrested cells in G2/M phase. In addition, suppression of CSTB induced cell apoptosis. In conclusion, CSTB was overexpressed and miR‑143‑3p was downregulated in ovarian malignant tumors. Mature miR‑143‑3p directly bound CSTB 3'‑UTR, leading to a decrease in CSTB expression in OC cells, which was regulated by TGF‑β1. Our findings suggest the potential therapeutic application of targeting the TGF‑β/miR‑143‑3p/CSTB axis for treating patients with OC.
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Copy and paste a formatted citation
Spandidos Publications style
Guan W, Wang X, Lin Q, Zhang J, Ren W and Xu G: Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer. Int J Oncol 55: 267-276, 2019.
APA
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., & Xu, G. (2019). Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer. International Journal of Oncology, 55, 267-276. https://doi.org/10.3892/ijo.2019.4815
MLA
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., Xu, G."Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer". International Journal of Oncology 55.1 (2019): 267-276.
Chicago
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., Xu, G."Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer". International Journal of Oncology 55, no. 1 (2019): 267-276. https://doi.org/10.3892/ijo.2019.4815
Copy and paste a formatted citation
x
Spandidos Publications style
Guan W, Wang X, Lin Q, Zhang J, Ren W and Xu G: Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer. Int J Oncol 55: 267-276, 2019.
APA
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., & Xu, G. (2019). Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer. International Journal of Oncology, 55, 267-276. https://doi.org/10.3892/ijo.2019.4815
MLA
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., Xu, G."Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer". International Journal of Oncology 55.1 (2019): 267-276.
Chicago
Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., Xu, G."Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer". International Journal of Oncology 55, no. 1 (2019): 267-276. https://doi.org/10.3892/ijo.2019.4815
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