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Article

Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo

  • Authors:
    • Jianwen Ye
    • Lei Qi
    • Kunlun Chen
    • Renfeng Li
    • Shengping Song
    • Chuang Zhou
    • Wenlong Zhai
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 331-339
    |
    Published online on: May 31, 2019
       https://doi.org/10.3892/ijo.2019.4820
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Abstract

Thyroid cancer is among the most common types of malignant tumor of the endocrine system. The role of metformin in the inhibition of cancer cell proliferation and induction of apoptosis is widely accepted. The present study explored the effect and the underlying mechanisms of metformin on human thyroid cancer TPC‑1 cells. Following treatment of TPC‑1 cells with different concentrations of metformin, cell proliferation and apoptosis were analyzed by cell counting kit‑8 (CCK‑8) assay and flow cytometry, respectively. Reverse transcription‑quantitative PCR and western blotting were used to detect alterations in the mRNA and protein expression levels, respectively, for heat shock protein family A member 5 (HSPA5, also known as Bip), DNA damage‑inducible transcript 3 (DDIT3, also known as CHOP) and caspase‑12. The results demonstrated that treatment with metformin inhibited proliferation and induced apoptosis in a concentration and time‑dependent manner. In addition, treatment with metformin increased the expression of Bip, CHOP and caspase‑12 in vitro, activating endoplasmic reticulum (ER) stress. Thapsigargin treatment enhanced the apoptosis induced by metformin. Inhibition of ER stress by 4‑phenylbutyrate reversed the metformin‑induced apoptosis. Finally, treatment with metformin inhibited thyroid cancer growth and increased the expression of Bip and CHOP in a TPC‑1 cell xenograft model. These results indicated that metformin increased the apoptotic rate of thyroid cancer cells via ER stress‑associated mechanisms.
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View References

1 

Xu Y, Zheng X, Qiu Y, Jia W, Wang J and Yin S: Distinct Metabolomic Profiles of Papillary Thyroid Carcinoma and Benign Thyroid Adenoma. J Proteome Res. 14:3315–3321. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Xing M: Molecular pathogenesis and mechanisms of thyroid cancer. Nat Rev Cancer. 13:184–199. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Zhou Z, Liu Y, Ma M and Chang L: Knockdown of TRIM44 inhibits the proliferation and invasion in papillary thyroid cancer cells through suppressing the Wnt/β-catenin signaling pathway. Biomed Pharmacother. 96:98–103. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Han G, Gong H, Wang Y, Guo S and Liu K: AMPK/mTOR-mediated inhibition of survivin partly contributes to metformin-induced apoptosis in human gastric cancer cell. Cancer Biol Ther. 16:77–87. 2015. View Article : Google Scholar :

5 

Feng T, Li L, Ling S, Fan N, Fang M, Zhang H, Fang X, Lan W, Hou Z, Meng Q, et al: Metformin enhances radiation response of ECa109 cells through activation of ATM and AMPK. Biomed Pharmacother. 69:260–266. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Qu C, Zhang W, Zheng G, Zhang Z, Yin J and He Z: Metformin reverses multidrug resistance and epithelial-mesenchymal transition (EMT) via activating AMP-activated protein kinase (AMPK) in human breast cancer cells. Mol Cell Biochem. 386:63–71. 2014. View Article : Google Scholar

7 

Chung HH, Moon JS, Yoon JS, Lee HW and Won KC: The relationship between metformin and cancer in patients with type 2 diabetes. Diabetes Metab J. 37:125–131. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Hall C, Stone RL, Gehlot A, Zorn KK and Burnett AF: Use of metformin in obese women with type I endometrial cancer is associated with a reduced incidence of cancer recurrence. Int J Gynecol Cancer. 26:313–317. 2016. View Article : Google Scholar

9 

Zhang X, Zhang X, Huang T, Geng J, Liu M and Zheng J: Combination of metformin and valproic acid synergistically induces cell cycle arrest and apoptosis in clear cell renal cell carcinoma. Int J Clin Exp Pathol. 8:2823–2828. 2015.PubMed/NCBI

10 

Chen X, Hu C, Zhang W, Shen Y, Wang J, Hu F and Yu P: Metformin inhibits the proliferation, metastasis, and cancer stem-like sphere formation in osteosarcoma MG63 cells in vitro. Tumour Biol. 36:9873–9883. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Bikas A, Jensen K, Patel A, Costello J Jr, McDaniel D, Klubo-Gwiezdzinska J, Larin O, Hoperia V, Burman KD, Boyle L, et al: Glucose-deprivation increases thyroid cancer cells sensitivity to metformin. Endocr Relat Cancer. 22:919–932. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Jung TW, Lee MW, Lee YJ and Kim SM: Metformin prevents endoplasmic reticulum stress-induced apoptosis through AMPK-PI3K-c-Jun NH2 pathway. Biochem Biophys Res Commun. 417:147–152. 2012. View Article : Google Scholar

13 

Sun X, Liao W, Wang J, Wang P, Gao H, Wang M, Xu C, Zhong Y and Ding Y: CSTMP induces apoptosis and mitochondrial dysfunction in human myeloma RPMI8226 cells via CHOP-dependent endoplasmic reticulum stress. Biomed Pharmacother. 83:776–784. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Granato M, Gilardini Montani MS, Romeo MA, Santarelli R, Gonnella R, D'Orazi G, Faggioni A and Cirone M: Metformin triggers apoptosis in PEL cells and alters bortezomib-induced Unfolded Protein Response increasing its cytotoxicity and inhibiting KSHV lytic cycle activation. Cell Signal. 40:239–247. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

16 

Brodowska K, Theodoropoulou S, Meyer Zu Hörste M, Paschalis EI, Takeuchi K, Scott G, Ramsey DJ, Kiernan E, Hoang M, Cichy J, et al: Effects of metformin on retinoblastoma growth in vitro and in vivo. Int J Oncol. 45:2311–2324. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Rico M, Baglioni M, Bondarenko M, Laluce NC, Rozados V, André N, Carré M, Scharovsky OG and Menacho Márquez M: Metformin and propranolol combination prevents cancer progression and metastasis in different breast cancer models. Oncotarget. 8:2874–2889. 2017. View Article : Google Scholar :

18 

Ye J, Chen K, Qi L, Li R, Tang H, Zhou C and Zhai W: Metformin suppresses hypoxia induced migration via the HIF 1α/VEGF pathway in gallbladder cancer in vitro and in vivo. Oncol Rep. 40:3501–3510. 2018.PubMed/NCBI

19 

He W, He S, Wang Z, Shen H, Fang W, Zhang Y, Qian W, Lin M, Yuan J, Wang J, et al: Astrocyte elevated gene-1(AEG-1) induces epithelial-mesenchymal transition in lung cancer through activating Wnt/β-catenin signaling. BMC Cancer. 15:1072015. View Article : Google Scholar

20 

Suwei D, Liang Z, Zhimin L, Ruilei L, Yingying Z, Zhen L, Chunlei G, Zhangchao L, Yuanbo X, Jinyan Y, et al: NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol. 8:1202015. View Article : Google Scholar : PubMed/NCBI

21 

Davies G, Lobanova L, Dawicki W, Groot G, Gordon JR, Bowen M, Harkness T and Arnason T: Metformin inhibits the development, and promotes the resensitization, of treatment-resistant breast cancer. PLoS One. 12:e01871912017. View Article : Google Scholar : PubMed/NCBI

22 

Xu Y, Li D, Zeng L, Wang C, Zhang L, Wang Y, Yu Y, Liu S and Li Z: Proteasome inhibitor lactacystin enhances cisplatin cyto-toxicity by increasing endoplasmic reticulum stress-associated apoptosis in HeLa cells. Mol Med Rep. 11:189–195. 2015. View Article : Google Scholar

23 

Wang J, Gao Q, Wang D, Wang Z and Hu C: Metformin inhibits growth of lung adenocarcinoma cells by inducing apoptosis via the mitochondria-mediated pathway. Oncol Lett. 10:1343–1349. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Reni M, Dugnani E, Cereda S, Belli C, Balzano G, Nicoletti R, Liberati D, Pasquale V, Scavini M, Maggiora P, et al: (Ir)relevance of metformin treatment in patients with metastatic pancreatic cancer: An open-label, randomized phase II trial. Clin Cancer Res. 22:1076–1085. 2016. View Article : Google Scholar

25 

Coperchini F, Leporati P, Rotondi M and Chiovato L: Expanding the therapeutic spectrum of metformin: From diabetes to cancer. J Endocrinol Invest. 38:1047–1055. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Cai H, Zhang Y, Han TK, Everett RS and Thakker DR: Cation-selective transporters are critical to the AMPK-mediated antiproliferative effects of metformin in human breast cancer cells. Int J Cancer. 138:2281–2292. 2016. View Article : Google Scholar

27 

Mert I, Chhina J, Allo G, Dai J, Seward S, Carey MS, Llaurado M, Giri S, Rattan R and Munkarah AR: Synergistic effect of MEK inhibitor and metformin combination in low grade serous ovarian cancer. Gynecol Oncol. 146:319–326. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Su Q, Tao T, Tang L, Deng J, Darko KO, Zhou S, Peng M, He S, Zeng Q, Chen AF, et al: Down-regulation of PKM2 enhances anticancer efficiency of THP on bladder cancer. J Cell Mol Med. 22:2774–2790. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Mynhardt C, Damelin LH, Jivan R, Peres J, Prince S, Veale RB and Mavri-Damelin D: Metformin-induced alterations in nucleotide metabolism cause 5-fluorouracil resistance but gemcitabine susceptibility in oesophageal squamous cell carcinoma. J Cell Biochem. 119:1193–1203. 2018. View Article : Google Scholar

30 

Tang JC, An R, Jiang YQ and Yang J: Effects and mechanisms of metformin on the proliferation of esophageal cancer cells in vitro and in vivo. Cancer Res Treat. 49:778–789. 2017. View Article : Google Scholar :

31 

Zhang J, Li G, Chen Y, Fang L, Guan C, Bai F, Ma M, Lyu J and Meng QH: Metformin inhibits tumorigenesis and tumor growth of breast cancer cells by upregulating miR-200c but downregu-lating AKT2 expression. J Cancer. 8:1849–1864. 2017. View Article : Google Scholar :

32 

Zhang J, Feng Z, Wang C, Zhou H, Liu W, Kanchana K, Dai X, Zou P, Gu J, Cai L, et al: Curcumin derivative WZ35 efficiently suppresses colon cancer progression through inducing ROS production and ER stress-dependent apoptosis. Am J Cancer Res. 7:275–288. 2017.PubMed/NCBI

33 

Martins AS, Alves I, Helguero L, Domingues MR and Neves BM: The Unfolded Protein Response in Homeostasis and Modulation of Mammalian Immune Cells. Int Rev Immunol. 35:457–476. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Hetz C, Chevet E and Harding HP: Targeting the unfolded protein response in disease. Nat Rev Drug Discov. 12:703–719. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Wu SM, Lin WY, Shen CC, Pan HC, Keh-Bin W, Chen YC, Jan YJ, Lai DW, Tang SC, Tien HR, et al: Melatonin set out to ER stress signaling thwarts epithelial mesenchymal transition and peritoneal dissemination via calpain-mediated C/EBPβ and NFκB cleavage. J Pineal Res. 60:142–154. 2016. View Article : Google Scholar

36 

Fan L, Song B, Sun G, Ma T, Zhong F and Wei W: Endoplasmic reticulum stress-induced resistance to doxorubicin is reversed by paeonol treatment in human hepatocellular carcinoma cells. PLoS One. 8:e626272013. View Article : Google Scholar : PubMed/NCBI

37 

KoraMagazi A, Wang D, Yousef B, Guerram M and Yu F: Rhein triggers apoptosis via induction of endoplasmic reticulum stress, caspase-4 and intracellular calcium in primary human hepatic HL-7702 cells. Biochem Biophys Res Commun. 473:230–236. 2016. View Article : Google Scholar : PubMed/NCBI

38 

Chen MC, Hsu HH, Chu YY, Cheng SF, Shen CY, Lin YJ, Chen RJ, Viswanadha VP, Lin YM and Huang CY: Lupeol alters ER stress-signaling pathway by downregulating ABCG2 expression to induce Oxaliplatin-resistant LoVo colorectal cancer cell apoptosis. Environ Toxicol. 33:587–593. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Lin CL, Lee CH, Chen CM, Cheng CW, Chen PN, Ying TH and Hsieh YH: Protodioscin induces apoptosis through ROS-mediated endoplasmic reticulum stress via the JNK/p38 activation pathways in human cervical cancer cells. Cell Physiol Biochem. 46:322–334. 2018. View Article : Google Scholar : PubMed/NCBI

40 

Wang G, Yang ZQ and Zhang K: Endoplasmic reticulum stress response in cancer: Molecular mechanism and therapeutic potential. Am J Transl Res. 2:65–74. 2010.PubMed/NCBI

41 

Malhi H and Kaufman RJ: Endoplasmic reticulum stress in liver disease. J Hepatol. 54:795–809. 2011. View Article : Google Scholar

42 

Cho SW, Yi KH, Han SK, Sun HJ, Kim YA, Oh BC, Park YJ and Park DJ: Therapeutic potential of metformin in papillary thyroid cancer in vitro and in vivo. Mol Cell Endocrinol. 393:24–29. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Thakur S, Daley B, Gaskins K, Vasko VV, Boufraqech M, Patel D, Sourbier C, Reece J, Cheng SY, Kebebew E, et al: Metformin targets mitochondrial glycerophosphate dehydrogenase to control rate of oxidative phosphorylation and growth of thyroid cancer in vitro and in vivo. Clin Cancer Res. 24:4030–4043. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Morales DR and Morris AD: Metformin in cancer treatment and prevention. Annu Rev Med. 66:17–29. 2015. View Article : Google Scholar

45 

Yang J, Wei J, Wu Y, Wang Z, Guo Y, Lee P and Li X: Metformin induces ER stress-dependent apoptosis through miR-708-5p/NNAT pathway in prostate cancer. Oncogenesis. 4:e1582015. View Article : Google Scholar : PubMed/NCBI

46 

Wang GY, Bi YG, Liu XD, Zhao Y, Han JF, Wei M and Zhang QY: Autophagy was involved in the protective effect of metformin on hyperglycemia-induced cardiomyocyte apoptosis and Connexin43 downregulation in H9c2 cells. Int J Med Sci. 14:698–704. 2017. View Article : Google Scholar : PubMed/NCBI

47 

Xiao Z, Gaertner S, Morresi-Hauf A, Genzel R, Duell T, Ullrich A and Knyazev PG: Metformin triggers autophagy to attenuate drug-induced apoptosis in NSCLC cells, with minor effects on tumors of diabetic patients. Neoplasia. 19:385–395. 2017. View Article : Google Scholar : PubMed/NCBI

48 

Li J, Gui Y, Ren J, Liu X, Feng Y, Zeng Z, He W, Yang J and Dai C: Metformin protects against cisplatin-induced tubular cell apoptosis and acute kidney injury via AMPKα-regulated autophagy induction. Sci Rep. 6:239752016. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ye J, Qi L, Chen K, Li R, Song S, Zhou C and Zhai W: Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo. Int J Oncol 55: 331-339, 2019.
APA
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., & Zhai, W. (2019). Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo. International Journal of Oncology, 55, 331-339. https://doi.org/10.3892/ijo.2019.4820
MLA
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., Zhai, W."Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo". International Journal of Oncology 55.1 (2019): 331-339.
Chicago
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., Zhai, W."Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo". International Journal of Oncology 55, no. 1 (2019): 331-339. https://doi.org/10.3892/ijo.2019.4820
Copy and paste a formatted citation
x
Spandidos Publications style
Ye J, Qi L, Chen K, Li R, Song S, Zhou C and Zhai W: Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo. Int J Oncol 55: 331-339, 2019.
APA
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., & Zhai, W. (2019). Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo. International Journal of Oncology, 55, 331-339. https://doi.org/10.3892/ijo.2019.4820
MLA
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., Zhai, W."Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo". International Journal of Oncology 55.1 (2019): 331-339.
Chicago
Ye, J., Qi, L., Chen, K., Li, R., Song, S., Zhou, C., Zhai, W."Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo". International Journal of Oncology 55, no. 1 (2019): 331-339. https://doi.org/10.3892/ijo.2019.4820
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