Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
October-2019 Volume 55 Issue 4

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2019 Volume 55 Issue 4

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells

  • Authors:
    • Atsushi Shiozaki
    • Yuzo Yamazato
    • Toshiyuki Kosuga
    • Michihiro Kudou
    • Katsutoshi Shoda
    • Tomohiro Arita
    • Hirotaka Konishi
    • Shuhei Komatsu
    • Takeshi Kubota
    • Hitoshi Fujiwara
    • Kazuma Okamoto
    • Yoshinori Marunaka
    • Eigo Otsuji
  • View Affiliations / Copyright

    Affiliations: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Kyoto 602‑8566, Japan, Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Kyoto 602‑8566, Japan
  • Pages: 905-914
    |
    Published online on: August 2, 2019
       https://doi.org/10.3892/ijo.2019.4853
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Although peritoneal lavage with distilled water performed after surgery prevents peritoneal seeding, cancer cells may avoid rupture under mild hypotonicity through regulatory volume decrease (RVD), which is the homeostatic regulation of ion and water transport. The aim of the present study was to investigate the effect of low temperature on cell volume and cell death under hypoosmolal conditions and determine the underlying molecular mechanisms in gastric cancer (GC). Three human GC cell lines (NUGC4, KATO‑III and MKN45) were exposed to hypotonic solutions, and the effects of low temperature on cell volume and viability were examined. Low temperature‑induced changes in membrane transporters were evaluated, and knockdown and overexpression experiments were conducted to determine their effects on cell volume during hypotonic stimulation. Low temperature (24˚C) during hypotonic stimulation inhibited RVD and enhanced the cytocidal effects on GC cells. The expression of leucine‑rich repeat containing protein A (LRRC8A), a component of a Cl‑ channel, was decreased, and aquaporin 5 (AQP5) expression was increased at low temperatures. LRRC8A knockdown markedly slowed the decrease in cell volume following cell swelling by hypotonic shock. AQP5 overexpression enhanced initial cell swelling after hypotonic shock and increased the final cell volume. These results suggest that a hypotonic solution at low temperature increased initial water influx via activation of AQP5 and decreased Cl‑ efflux via inhibition of LRRC8A. Therefore, low temperature enhanced the hypotonicity‑induced cytocidal effects on GC cells, and these results may contribute to the development of a novel lavage method effective in reducing peritoneal recurrence in GC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Lim L, Michael M, Mann GB and Leong T: Adjuvant therapy in gastric cancer. J Clin Oncol. 23:6220–6232. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Rajdev L: Treatment options for surgically resectable gastric cancer. Curr Treat Options Oncol. 11:14–23. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Hartgrink HH, Jansen EP, van Grieken NC and van de Velde CJ: Gastric cancer. Lancet. 374:477–490. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Emoto S, Yamaguchi H, Kishikawa J, Yamashita H, Ishigami H and Kitayama J: Antitumor effect and pharmacokinetics of intraperitoneal NK105, a nanomicellar paclitaxel formulation for peritoneal dissemination. Cancer Sci. 103:1304–1310. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Shiozaki A, Ichikawa D, Kosuga T, Marunaka Y and Otsuji E: Regulation of osmolality for cancer treatment. J Physiol Sci. 67:353–360. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Park KG, Chetty U, Scott W and Miller W: The activity of locally applied cytotoxics to breast cancer cells in vitro. Ann R Coll Surg Engl. 73:96–99. 1991.PubMed/NCBI

7 

Huguet EL and Keeling NJ: Distilled water peritoneal lavage after colorectal cancer surgery. Dis Colon Rectum. 47:2114–2119. 2004. View Article : Google Scholar

8 

Zhou SJ, Zhang EL, Liang BY, Zhang ZY, Chen XP and Huang ZY: Distilled water lavage during surgery improves long-term outcomes of patients with ruptured hepatocellular carcinoma. J Gastrointest Surg. 19:1262–1270. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Fechner G, Pocha K, Schmidt D and Müller SC: Reducing recurrence and costs in superficial bladder cancer: Preclinical evaluation of osmotic cytolysis by distilled water vs. mitomycin. Int J Clin Pract. 60:1178–1180. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Caplanusi A, Kim KJ, Lariviere E, Van Driessche W and Jans D: Swelling-activated K+ efflux and regulatory volume decrease efficiency in human bronchial epithelial cells. J Membr Biol. 214:33–41. 2006. View Article : Google Scholar

11 

Miyazaki H, Shiozaki A, Niisato N and Marunaka Y: Physiological significance of hypotonicity-induced regulatory volume decrease: Reduction in intracellular Cl- concentration acting as an intracellular signaling. Am J Physiol Renal Physiol. 292:F1411–F1417. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, et al: Blockade of chloride ion transport enhances the cytocidal effect of hypotonic solution in gastric cancer cells. J Surg Res. 176:524–534. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Kosuga T, Shiozaki A, Ichikawa D, Fujiwara H, Komatsu S, Iitaka D, Tsujiura M, Morimura R, Takeshita H, Nagata H, et al: Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma. Oncol Rep. 26:577–586. 2011.PubMed/NCBI

14 

Nako Y, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Iitaka D, Ishii H, Ikoma H, Kubota T, Fujiwara H, et al: Enhancement of the cytocidal effects of hypotonic solution using a chloride channel blocker in pancreatic cancer cells. Pancreatology. 12:440–448. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Takemoto K, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Nako Y, Murayama Y, Kuriu Y, Nakanishi M, Fujiwara H, et al: Evaluation of the efficacy of peritoneal lavage with distilled water in colorectal cancer surgery: In vitro and in vivo study. J Gastroenterol. 50:287–297. 2015. View Article : Google Scholar

16 

Kudou M, Shiozaki A, Kosuga T, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, Okamoto K, et al: Inhibition of regulatory volume decrease enhances the cytocidal effect of hypotonic shock in hepatocellular carcinoma. J Cancer. 7:1524–1533. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Kudou M, Shiozaki A, Kosuga T, Shimizu H, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, et al: Heat shock exerts anticancer effects on liver cancer via autophagic degradation of aquaporin 5. Int J Oncol. 50:1857–1867. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Bünger S, Roblick UJ and Habermann JK: Comparison of five commercial extraction kits for subsequent membrane protein profiling. Cytotechnology. 61:153–159. 2009. View Article : Google Scholar

19 

Liu Y, Belkina NV, Park C, Nambiar R, Loughhead SM, Patino-Lopez G, Ben-Aissa K, Hao JJ, Kruhlak MJ, Qi H, et al: Constitutively active ezrin increases membrane tension, slows migration, and impedes endothelial transmigration of lymphocytes in vivo in mice. Blood. 119:445–453. 2012. View Article : Google Scholar :

20 

Kédinger V, Alpy F, Baguet A, Polette M, Stoll I, Chenard MP, Tomasetto C and Rio MC: Tumor necrosis factor receptor-associated factor 4 is a dynamic tight junction-related shuttle protein involved in epithelium homeostasis. PLoS One. 3:e35182008. View Article : Google Scholar : PubMed/NCBI

21 

Shiozaki A, Ichikawa D, Takemoto K, Nako Y, Nakashima S, Shimizu H, Kitagawa M, Kosuga T, Konishi H, Komatsu S, et al: Efficacy of a hypotonic treatment for peritoneal dissemination from gastric cancer cells: An in vivo evaluation. BioMed Res Int. 2014:7070892014. View Article : Google Scholar : PubMed/NCBI

22 

Whiteside OJ, Tytherleigh MG, Thrush S, Farouk R and Galland RB: Intra-operative peritoneal lavage--who does it and why? Ann R Coll Surg Engl. 87:255–258. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Lin CH, Hsieh HF, Yu JC, Chen TW, Yu CY and Hsieh CB: Peritoneal lavage with distilled water during liver resection in patients with spontaneously ruptured hepatocellular carcinomas. J Surg Oncol. 94:255–256. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Chang YM, Hsu KF, Yu JC, Chan DC, Chen CJ, Chen TW, Hsieh CB and Hsieh HF: Distilled water peritoneal lavage in patients with rupture hepatocellular carcinoma. Hepatogastroenterology. 60:140–143. 2013.PubMed/NCBI

25 

Ichinose Y, Tsuchiya R, Koike T, Yasumitsu T, Nakamura K, Tada H, Yoshimura H, Mitsudomi T, Nakagawa K, Yokoi K, et al: A prematurely terminated phase III trial of intraoperative intra-pleural hypotonic cisplatin treatment in patients with resected non-small cell lung cancer with positive pleural lavage cytology: The incidence of carcinomatous pleuritis after surgical intervention. J Thorac Cardiovasc Surg. 123:695–699. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Tsujitani S, Fukuda K, Saito H, Kondo A, Ikeguchi M, Maeta M and Kaibara N: The administration of hypotonic intraperitoneal cisplatin during operation as a treatment for the peritoneal dissemination of gastric cancer. Surgery. 131(Suppl): S98–S104. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Seto T, Ushijima S, Yamamoto H, Ito K, Araki J, Inoue Y, Semba H and Ichinose Y; Thoracic Oncology Group: Intrapleural hypotonic cisplatin treatment for malignant pleural effusion in 80 patients with non-small-cell lung cancer: A multi-institutional phase II trial. Br J Cancer. 95:717–721. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Ohki A, Abe N, Yoshimoto E, Hashimoto Y, Takeuchi H, Nagao G, Masaki T, Mori T, Ohkura Y and Sugiyama M: Gastric washing by distilled water can reduce free gastric cancer cells exfoliated into the stomach lumen. Gastric Cancer. 21:998–1003. 2018. View Article : Google Scholar : PubMed/NCBI

29 

McManus ML, Churchwell KB and Strange K: Regulation of cell volume in health and disease. N Engl J Med. 333:1260–1266. 1995. View Article : Google Scholar : PubMed/NCBI

30 

Strange K, Emma F and Jackson PS: Cellular and molecular physiology of volume-sensitive anion channels. Am J Physiol. 270:C711–C730. 1996. View Article : Google Scholar : PubMed/NCBI

31 

Souza MM and Boyle RT: A moderate decrease in temperature inhibits the calcium signaling mechanism(s) of the regulatory volume decrease in chick embryo cardiomyocytes. Braz J Med Biol Res. 34:137–141. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Qiu Z, Dubin AE, Mathur J, Tu B, Reddy K, Miraglia LJ, Reinhardt J, Orth AP and Patapoutian A: SWELL1, a plasma membrane protein, is an essential component of volume-regulated anion channel. Cell. 157:447–458. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Okada Y, Okada T, Sato-Numata K, Islam MR, Ando-Akatsuka Y, Numata T, Kubo M, Shimizu T, Kurbannazarova RS, Marunaka Y, et al: Cell volume-activated and volume-correlated anion channels in mammalian cells: Their biophysical, molecular, and pharmacological properties. Pharmacol Rev. 71:49–88. 2019. View Article : Google Scholar

34 

Ishibashi K, Hara S and Kondo S: Aquaporin water channels in mammals. Clin Exp Nephrol. 13:107–117. 2009. View Article : Google Scholar

35 

Day RE, Kitchen P, Owen DS, Bland C, Marshall L, Conner AC, Bill RM and Conner MT: Human aquaporins: Regulators of transcellular water flow. Biochim Biophys Acta. 1840:1492–1506. 2014. View Article : Google Scholar

36 

Hoffmann EK, Lambert IH and Pedersen SF: Physiology of cell volume regulation in vertebrates. Physiol Rev. 89:193–277. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Mola MG, Sparaneo A, Gargano CD, Spray DC, Svelto M, Frigeri A, Scemes E and Nicchia GP: The speed of swelling kinetics modulates cell volume regulation and calcium signaling in astrocytes: A different point of view on the role of aquaporins. Glia. 64:139–154. 2016. View Article : Google Scholar :

38 

Shiozaki A, Ichikawa D, Otsuji E and Marunaka Y: Cellular physiological approach for treatment of gastric cancer. World J Gastroenterol. 20:11560–11566. 2014. View Article : Google Scholar : PubMed/NCBI

39 

Shiozaki A, Miyazaki H, Niisato N, Nakahari T, Iwasaki Y, Itoi H, Ueda Y, Yamagishi H and Marunaka Y: Furosemide, a blocker of Na+/K+/2Cl- cotransporter, diminishes proliferation of poorly differentiated human gastric cancer cells by affecting G0/G1 state. J Physiol Sci. 56:401–406. 2006. View Article : Google Scholar : PubMed/NCBI

40 

Miyazaki H, Shiozaki A, Niisato N, Ohsawa R, Itoi H, Ueda Y, Otsuji E, Yamagishi H, Iwasaki Y, Nakano T, et al: Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells. Biochem Biophys Res Commun. 366:506–512. 2008. View Article : Google Scholar

41 

Shiozaki A, Otsuji E and Marunaka Y: Intracellular chloride regulates the G(1)/S cell cycle progression in gastric cancer cells. World J Gastrointest Oncol. 3:119–122. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Shiozaki A, Ariyoshi Y, Iitaka D, Kosuga T, Shimizu H, Kudou M, Konishi T, Shoda K, Arita T, Konishi H, et al: Functional analysis and clinical significance of sodium iodide symporter expression in gastric cancer. Gastric Cancer. 22:473–485. 2019. View Article : Google Scholar

43 

Kosuga T, Shiozaki A, Kudou M, Yamazato Y, Ichikawa D, Komatsu S, Konishi H, Okamoto K, Shoda K, Arita T, et al: Blockade of potassium ion transports enhances hypoto-nicity-induced cytocidal effects in gastric cancer. Oncotarget. 8:101394–101405. 2017. View Article : Google Scholar : PubMed/NCBI

44 

Koca YS, Tarhan OR, Kaya S and Gökçe Ceylan B: Effects of saline lavage temperature on peritoneal fibrinolysis and adhesion formation. Ulus Travma Acil Cerrahi Derg. 22:1–6. 2016.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shiozaki A, Yamazato Y, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Fujiwara H, et al: Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells. Int J Oncol 55: 905-914, 2019.
APA
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T. ... Otsuji, E. (2019). Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells. International Journal of Oncology, 55, 905-914. https://doi.org/10.3892/ijo.2019.4853
MLA
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T., Konishi, H., Komatsu, S., Kubota, T., Fujiwara, H., Okamoto, K., Marunaka, Y., Otsuji, E."Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells". International Journal of Oncology 55.4 (2019): 905-914.
Chicago
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T., Konishi, H., Komatsu, S., Kubota, T., Fujiwara, H., Okamoto, K., Marunaka, Y., Otsuji, E."Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells". International Journal of Oncology 55, no. 4 (2019): 905-914. https://doi.org/10.3892/ijo.2019.4853
Copy and paste a formatted citation
x
Spandidos Publications style
Shiozaki A, Yamazato Y, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Fujiwara H, et al: Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells. Int J Oncol 55: 905-914, 2019.
APA
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T. ... Otsuji, E. (2019). Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells. International Journal of Oncology, 55, 905-914. https://doi.org/10.3892/ijo.2019.4853
MLA
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T., Konishi, H., Komatsu, S., Kubota, T., Fujiwara, H., Okamoto, K., Marunaka, Y., Otsuji, E."Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells". International Journal of Oncology 55.4 (2019): 905-914.
Chicago
Shiozaki, A., Yamazato, Y., Kosuga, T., Kudou, M., Shoda, K., Arita, T., Konishi, H., Komatsu, S., Kubota, T., Fujiwara, H., Okamoto, K., Marunaka, Y., Otsuji, E."Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cells". International Journal of Oncology 55, no. 4 (2019): 905-914. https://doi.org/10.3892/ijo.2019.4853
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team