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UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials

  • Authors:
    • Di Feng
    • Jing Yuan
    • Qi Liu
    • Li Liu
    • Xu Zhang
    • Yali Wu
    • Yifan Qian
    • Liping Chen
    • Yan Shi
    • Mancang Gu
  • View Affiliations / Copyright

    Affiliations: College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 311402, P.R. China, Department of Pathology, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA, Department of Epidemiology and Biostatistics, Ministry of Education Key Lab of Environment and Health, School of Public Health, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 311402, P.R. China, Department of Medical Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1249-1260
    |
    Published online on: October 14, 2019
       https://doi.org/10.3892/ijo.2019.4898
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Abstract

The purpose of the present study was to compare metabolites from formalin‑fixed and paraffin‑embedded (FFPE) pancreatic tissue blocks with those identified in optimal cutting temperature (OCT)‑embedded pancreatic tissue blocks. Thus, ultra‑performance liquid chromatograph‑mass spectrometry/mass spectrometry‑based metabolic profiling was performed in paired frozen (n=13) and FFPE (n=13) human pancreatic adenocarcinoma tissue samples, in addition to their benign counterparts. A total of 206 metabolites were identified in both OCT‑embedded and FFPE tissue samples. The method feasibility was confirmed through reproducibility and a consistency assessment. Partial least‑squares discriminant analysis and heatmap analysis reliably distinguished tumor and normal tissue phenotypes. The expression of 10 compounds, including N‑acetylaspartate and creatinine, was significantly different in both OCT‑embedded and FFPE tumor samples. These ten compounds may be viable candidate biomarkers of malignant pancreatic tissues. The super‑categories to which they belonged exhibited no significant differences between FFPE and OCT‑embedded samples. Furthermore, purine, arginine and proline, and pyrimidine metabolism used a shared pathway found in both OCT‑embedded and FFPE tissue samples. These results supported the notion that metabolomic data acquired from FFPE pancreatic cancer specimens are reliable for use in retrospective and clinical studies.
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Copy and paste a formatted citation
Spandidos Publications style
Feng D, Yuan J, Liu Q, Liu L, Zhang X, Wu Y, Qian Y, Chen L, Shi Y, Gu M, Gu M, et al: UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials. Int J Oncol 55: 1249-1260, 2019.
APA
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y. ... Gu, M. (2019). UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials. International Journal of Oncology, 55, 1249-1260. https://doi.org/10.3892/ijo.2019.4898
MLA
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y., Qian, Y., Chen, L., Shi, Y., Gu, M."UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials". International Journal of Oncology 55.6 (2019): 1249-1260.
Chicago
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y., Qian, Y., Chen, L., Shi, Y., Gu, M."UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials". International Journal of Oncology 55, no. 6 (2019): 1249-1260. https://doi.org/10.3892/ijo.2019.4898
Copy and paste a formatted citation
x
Spandidos Publications style
Feng D, Yuan J, Liu Q, Liu L, Zhang X, Wu Y, Qian Y, Chen L, Shi Y, Gu M, Gu M, et al: UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials. Int J Oncol 55: 1249-1260, 2019.
APA
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y. ... Gu, M. (2019). UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials. International Journal of Oncology, 55, 1249-1260. https://doi.org/10.3892/ijo.2019.4898
MLA
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y., Qian, Y., Chen, L., Shi, Y., Gu, M."UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials". International Journal of Oncology 55.6 (2019): 1249-1260.
Chicago
Feng, D., Yuan, J., Liu, Q., Liu, L., Zhang, X., Wu, Y., Qian, Y., Chen, L., Shi, Y., Gu, M."UPLC‑MS/MS‑based metabolomic characterization and comparison of pancreatic adenocarcinoma tissues using formalin‑fixed, paraffin‑embedded and optimal cutting temperature‑embedded materials". International Journal of Oncology 55, no. 6 (2019): 1249-1260. https://doi.org/10.3892/ijo.2019.4898
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