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Article

Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells

  • Authors:
    • Rafal Szatanek
    • Kazimierz Weglarczyk
    • Malgorzata Stec
    • Jaroslaw Baran
    • Magdalena Parlinska‑Wojtan
    • Maciej Siedlar
    • Monika Baj‑Krzyworzeka
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Immunology, Jagiellonian University Medical College, 30‑663 Krakow, Poland, Institute of Nuclear Physics Polish Academy of Sciences, 31‑342 Krakow, Poland
  • Pages: 359-367
    |
    Published online on: November 26, 2019
       https://doi.org/10.3892/ijo.2019.4923
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Abstract

Tumor‑derived microvesicles (TMVs) interact with a variety of different cell types within the immune system, including lymphocytes, monocytes, dendritic cells and tumor cells that they have originated from. In the present study, the effects of autologous‑TMVs (auto‑TMVs) on gene expression, chemotaxis, intercellular signaling and cellular metabolism were examined in cells of the gastric cancer (GC) cell line 1415 (GC1415). The effects of auto‑TMVs on mRNA gene expression in GC1415 cells were assessed using pathway‑focused PCR arrays. A chemotaxis assay was performed using the HoloMonitor M4 System. Signaling pathways were evaluated using western blot analysis, and cellular respiration was measured using the Seahorse XF Cell Mito Stress Test. Exposure of the GC1415 cells to auto‑TMVs led to the overexpression (75 genes) and underexpression (96 genes) of genes that are associated with signal transduction, metabolism, chemotaxis, angiogenesis and metastasis. The auto‑TMVs were indicated to induce chemotaxis and activate the PI3K/AKT signaling pathway in GC1415 cells. However, the MAPK/ERK signaling pathway was not indicated to be activated. Furthermore, studies on cellular respiration in GC1415 cells exposed to auto‑TMVs demonstrated a metabolic shift to glycolysis. The results of the current study thus indicate that auto‑TMVs may exert an effect on tumor cell function.
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Copy and paste a formatted citation
Spandidos Publications style
Szatanek R, Weglarczyk K, Stec M, Baran J, Parlinska‑Wojtan M, Siedlar M and Baj‑Krzyworzeka M: Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells. Int J Oncol 56: 359-367, 2020.
APA
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., & Baj‑Krzyworzeka, M. (2020). Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells. International Journal of Oncology, 56, 359-367. https://doi.org/10.3892/ijo.2019.4923
MLA
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., Baj‑Krzyworzeka, M."Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells". International Journal of Oncology 56.1 (2020): 359-367.
Chicago
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., Baj‑Krzyworzeka, M."Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells". International Journal of Oncology 56, no. 1 (2020): 359-367. https://doi.org/10.3892/ijo.2019.4923
Copy and paste a formatted citation
x
Spandidos Publications style
Szatanek R, Weglarczyk K, Stec M, Baran J, Parlinska‑Wojtan M, Siedlar M and Baj‑Krzyworzeka M: Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells. Int J Oncol 56: 359-367, 2020.
APA
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., & Baj‑Krzyworzeka, M. (2020). Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells. International Journal of Oncology, 56, 359-367. https://doi.org/10.3892/ijo.2019.4923
MLA
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., Baj‑Krzyworzeka, M."Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells". International Journal of Oncology 56.1 (2020): 359-367.
Chicago
Szatanek, R., Weglarczyk, K., Stec, M., Baran, J., Parlinska‑Wojtan, M., Siedlar, M., Baj‑Krzyworzeka, M."Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells". International Journal of Oncology 56, no. 1 (2020): 359-367. https://doi.org/10.3892/ijo.2019.4923
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