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Article

Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma

  • Authors:
    • Venkatesh Chanukuppa
    • Debasish Paul
    • Khushman Taunk
    • Tathagata Chatterjee
    • Sanjeevan Sharma
    • Amey Shirolkar
    • Sehbanul Islam
    • Manas K. Santra
    • Srikanth Rapole
  • View Affiliations / Copyright

    Affiliations: Proteomics Laboratory, National Centre for Cell Science, Pune, Maharashtra 411007, India, Savitribai Phule Pune University, Pune, Maharashtra 411007, India, Army Hospital (Research and Referral), Dhaula Kuan, New Delhi, Delhi 110010, India , Armed Forces Medical College, Pune, Maharashtra 411001, India, Cancer Biology and Epigenetics Laboratory, National Centre for Cell Science, Pune, Maharashtra 411007, India
  • Pages: 325-337
    |
    Published online on: April 29, 2020
       https://doi.org/10.3892/ijo.2020.5056
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Abstract

Multiple myeloma (MM) is a plasma cell‑associated cancer and accounts for 13% of all hematological malignancies, worldwide. MM still remains an incurable plasma cell malignancy with a poor prognosis due to a lack of suitable markers. Therefore, discovering novel markers and targets for diagnosis and therapeutics of MM is essential. The present study aims to identify markers associated with MM malignancy using patient‑derived MM mononuclear cells (MNCs). Label‑free quantitative proteomics analysis revealed a total of 192 differentially regulated proteins, in which 79 proteins were upregulated and 113 proteins were found to be downregulated in MM MNCs as compared to non‑hematological malignant samples. The identified differentially expressed candidate proteins were analyzed using various bioinformatics tools, including Ingenuity Pathway Analysis (IPA), Protein Analysis THrough Evolutionary Relationships (PANTHER), Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Database for Annotation, Visualization and Integrated Discovery (DAVID) to determine their biological context. Among the 192 candidate proteins, marginal zone B and B1 cell specific protein (MZB1) was investigated in detail using the RPMI-8226 cell line model of MM. The functional studies revealed that higher expression of MZB1 is associated with promoting the progression of MM pathogenesis and could be established as a potential target for MM in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Chanukuppa V, Paul D, Taunk K, Chatterjee T, Sharma S, Shirolkar A, Islam S, Santra MK and Rapole S: Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma. Int J Oncol 57: 325-337, 2020.
APA
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A. ... Rapole, S. (2020). Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma. International Journal of Oncology, 57, 325-337. https://doi.org/10.3892/ijo.2020.5056
MLA
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A., Islam, S., Santra, M. K., Rapole, S."Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma". International Journal of Oncology 57.1 (2020): 325-337.
Chicago
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A., Islam, S., Santra, M. K., Rapole, S."Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma". International Journal of Oncology 57, no. 1 (2020): 325-337. https://doi.org/10.3892/ijo.2020.5056
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Spandidos Publications style
Chanukuppa V, Paul D, Taunk K, Chatterjee T, Sharma S, Shirolkar A, Islam S, Santra MK and Rapole S: Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma. Int J Oncol 57: 325-337, 2020.
APA
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A. ... Rapole, S. (2020). Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma. International Journal of Oncology, 57, 325-337. https://doi.org/10.3892/ijo.2020.5056
MLA
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A., Islam, S., Santra, M. K., Rapole, S."Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma". International Journal of Oncology 57.1 (2020): 325-337.
Chicago
Chanukuppa, V., Paul, D., Taunk, K., Chatterjee, T., Sharma, S., Shirolkar, A., Islam, S., Santra, M. K., Rapole, S."Proteomics and functional study reveal marginal zone B and B1 cell specific protein as a candidate marker of multiple myeloma". International Journal of Oncology 57, no. 1 (2020): 325-337. https://doi.org/10.3892/ijo.2020.5056
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