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MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4

  • Authors:
    • Yuanyuan Kang
    • Ying Zhang
    • Yan Sun
  • View Affiliations / Copyright

    Affiliations: Department of Emergency and Oral Medicine, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning 110002, P.R. China
    Copyright: © Kang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 39
    |
    Published online on: May 11, 2021
       https://doi.org/10.3892/ijo.2021.5219
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Abstract

MicroRNAs (miRNAs/miR) often contribute to the progression of oral squamous cell carcinoma (OSCC) via the regulation of mRNA. The present study aimed to investigate the role of miR‑198 in OSCC pathogenesis and explore the underlying mechanism. Reverse transcription‑quantitative (RT‑q)PCR was performed to determine miR‑198 expression in OSCC tissues and cell lines, and univariate and multivariate analyses were applied to evaluate the survival of patients with OSCC. The effects of miR‑198 on OSCC cell lines were studied in vitro and in vivo. A set of epithelial‑mesenchymal transition (EMT) markers were detected to determine whether miR‑198 was involved in EMT. Lastly, using luciferase assays, a novel target of miR‑198 was identified and the effect of the new target gene of miR‑198 on cell proliferation and invasion was also studied. It was identified that miR‑198 expression was decreased in OSCC tissues and cell lines, and low expression of miR‑198 was associated with poor overall survival and disease‑free survival. Overexpression of miR‑198 appeared to significantly inhibit the proliferation, invasion and EMT of OSCC cells. Moreover, the luciferase assay results showed that miR‑198 interacted with cyclin‑dependent kinase 4 (CDK4) by directly targeting the miRNA‑binding site in the CDK4 sequence, and RT‑qPCR results showed that CDK4 expression was increased in OSCC tissues and cell lines. In addition, transfection of small interfering RNA against CDK4 in OSCC cells showed similar inhibitory effects on cell proliferation, invasion and EMT, whereas CDK4 overexpression in OSCC cells partially reversed the inhibitory effects of the miR‑198 mimic. The present results indicated that miR‑198 suppressed OSCC tumour growth and metastasis by directly targeting CDK4 expression. Thus, miR‑198 may be a potential therapeutic target in the treatment of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Kang Y, Zhang Y and Sun Y: MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4. Int J Oncol 59: 39, 2021.
APA
Kang, Y., Zhang, Y., & Sun, Y. (2021). MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4. International Journal of Oncology, 59, 39. https://doi.org/10.3892/ijo.2021.5219
MLA
Kang, Y., Zhang, Y., Sun, Y."MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4". International Journal of Oncology 59.1 (2021): 39.
Chicago
Kang, Y., Zhang, Y., Sun, Y."MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4". International Journal of Oncology 59, no. 1 (2021): 39. https://doi.org/10.3892/ijo.2021.5219
Copy and paste a formatted citation
x
Spandidos Publications style
Kang Y, Zhang Y and Sun Y: MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4. Int J Oncol 59: 39, 2021.
APA
Kang, Y., Zhang, Y., & Sun, Y. (2021). MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4. International Journal of Oncology, 59, 39. https://doi.org/10.3892/ijo.2021.5219
MLA
Kang, Y., Zhang, Y., Sun, Y."MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4". International Journal of Oncology 59.1 (2021): 39.
Chicago
Kang, Y., Zhang, Y., Sun, Y."MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4". International Journal of Oncology 59, no. 1 (2021): 39. https://doi.org/10.3892/ijo.2021.5219
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