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Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival

  • Authors:
    • Maëva Chauvin
    • Véronique Garambois
    • Sylvie Choblet
    • Pierre-Emmanuel Colombo
    • Myriam Chentouf
    • Laurent Gros
    • David-Paul De Brauwere
    • Martine Duonor-Cerutti
    • Karen Dumas
    • Bruno Robert
    • Marta Jarlier
    • Pierre Martineau
    • Isabelle Navarro-Teulon
    • David Pépin
    • Thierry Chardès
    • André Pèlegrin
  • View Affiliations / Copyright

    Affiliations: IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université de Montpellier, Institut Régional du Cancer de Montpellier, F-34298 Montpellier, France, CNRS UPS3044 Baculovirus et Thérapie, F-30380 Saint-Christol-Lèz Alès, France, Département de Biochimie et Biologie Moléculaire, CHU de Nîmes, F-30900 Nîmes, France, SurgiMAb, F-34000 Montpellier, France, Institut Régional du Cancer de Montpellier, ICM, F-34298 Montpellier, France, Department of Surgery, Harvard Medical School, Boston, MA 02114, USA
    Copyright: © Chauvin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 43
    |
    Published online on: May 17, 2021
       https://doi.org/10.3892/ijo.2021.5223
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Abstract

Anti‑Müllerian hormone (AMH) type II receptor (AMHRII) and the AMH/AMHRII signaling pathway are potential therapeutic targets in ovarian carcinoma. Conversely, the role of the three AMH type I receptors (AMHRIs), namely activin receptor‑like kinase (ALK)2, ALK3 and ALK6, in ovarian cancer remains to be clarified. To determine the respective roles of these three AMHRIs, the present study used four ovarian cancer cell lines (COV434‑AMHRII, SKOV3‑AMHRII, OVCAR8, KGN) and primary cells isolated from tumor ascites from patients with ovarian cancer. The results demonstrated that ALK2 and ALK3 may be the two main AMHRIs involved in AMH signaling at physiological endogenous and supraphysiological exogenous AMH concentrations, respectively. Supraphysiological AMH concentrations (25 nM recombinant AMH) were associated with apoptosis in all four cell lines and decreased clonogenic survival in COV434‑AMHRII and SKOV3‑AMHRII cells. These biological effects were induced via ALK3 recruitment by AMHRII, as ALK3‑AMHRII dimerization was favored at increasing AMH concentrations. By contrast, ALK2 was associated with AMHRII at physiological endogenous concentrations of AMH (10 pM). Based on these results, tetravalent IgG1‑like bispecific antibodies (BsAbs) against AMHRII and ALK2, and against AMHRII and ALK3 were designed and evaluated. In vivo, COV434‑AMHRII tumor cell xenograft growth was significantly reduced in all BsAb‑treated groups compared with that in the vehicle group (P=0.018 for BsAb 12G4‑3D7; P=0.001 for all other BsAbs). However, the growth of COV434‑AMHRII tumor cell xenografts was slower in mice treated with the anti‑AMRII‑ALK2 BsAb 12G4‑2F9 compared with that in animals that received a control BsAb that targeted AMHRII and CD5 (P=0.048). These results provide new insights into type I receptor specificity in AMH signaling pathways and may lead to an innovative therapeutic approach to modulate AMH signaling using anti‑AMHRII/anti‑AMHRI BsAbs.
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Chauvin M, Garambois V, Choblet S, Colombo P, Chentouf M, Gros L, De Brauwere D, Duonor-Cerutti M, Dumas K, Robert B, Robert B, et al: Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival. Int J Oncol 59: 43, 2021.
APA
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L. ... Pèlegrin, A. (2021). Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival. International Journal of Oncology, 59, 43. https://doi.org/10.3892/ijo.2021.5223
MLA
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L., De Brauwere, D., Duonor-Cerutti, M., Dumas, K., Robert, B., Jarlier, M., Martineau, P., Navarro-Teulon, I., Pépin, D., Chardès, T., Pèlegrin, A."Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival". International Journal of Oncology 59.1 (2021): 43.
Chicago
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L., De Brauwere, D., Duonor-Cerutti, M., Dumas, K., Robert, B., Jarlier, M., Martineau, P., Navarro-Teulon, I., Pépin, D., Chardès, T., Pèlegrin, A."Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival". International Journal of Oncology 59, no. 1 (2021): 43. https://doi.org/10.3892/ijo.2021.5223
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Spandidos Publications style
Chauvin M, Garambois V, Choblet S, Colombo P, Chentouf M, Gros L, De Brauwere D, Duonor-Cerutti M, Dumas K, Robert B, Robert B, et al: Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival. Int J Oncol 59: 43, 2021.
APA
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L. ... Pèlegrin, A. (2021). Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival. International Journal of Oncology, 59, 43. https://doi.org/10.3892/ijo.2021.5223
MLA
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L., De Brauwere, D., Duonor-Cerutti, M., Dumas, K., Robert, B., Jarlier, M., Martineau, P., Navarro-Teulon, I., Pépin, D., Chardès, T., Pèlegrin, A."Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival". International Journal of Oncology 59.1 (2021): 43.
Chicago
Chauvin, M., Garambois, V., Choblet, S., Colombo, P., Chentouf, M., Gros, L., De Brauwere, D., Duonor-Cerutti, M., Dumas, K., Robert, B., Jarlier, M., Martineau, P., Navarro-Teulon, I., Pépin, D., Chardès, T., Pèlegrin, A."Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival". International Journal of Oncology 59, no. 1 (2021): 43. https://doi.org/10.3892/ijo.2021.5223
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