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Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma

  • Authors:
    • Qiusheng Shan
    • Kiyofumi Takabatake
    • Haruka Omori
    • Hotaka Kawai
    • May Wathone Oo
    • Keisuke Nakano
    • Soichiro Ibaragi
    • Akira Sasaki
    • Hitoshi Nagatsuka
  • View Affiliations / Copyright

    Affiliations: Department of Oral Pathology and Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Kita‑ku, Okayama 700‑8525, Japan, Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Kita‑ku, Okayama 700‑8525, Japan
    Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 72
    |
    Published online on: August 6, 2021
       https://doi.org/10.3892/ijo.2021.5252
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Abstract

The stromal cells in the tumor microenvironment (TME) can influence the progression of multiple types of cancer; however, data on oral squamous cell carcinoma (OSCC) are limited. In the present study, the effects of verrucous squamous cell carcinoma‑associated stromal cells (VSCC‑SCs), squamous cell carcinoma‑associated stromal cells (SCC‑SCs) and human dermal fibroblasts (HDFs) on the tumor nest formation, proliferation, invasion and migration of HSC‑3 cells were examined in vitro using Giemsa staining, MTS, and Transwell (invasion and migration) assays, respectively. The results revealed that both the VSCC‑SCs and SCC‑SCs inhibited the tumor nest formation, and promoted the proliferation, invasion and migration of OSCC cells in vitro. Furthermore, the effects of VSCC‑SCs, SCC‑SCs and HDFs on the differentiation, proliferation, invasion and migration of OSCC cells in vivo were evaluated by hematoxylin and eosin staining, tartrate‑resistant acid phosphatase staining, immunohistochemistry and double‑fluorescent immunohistochemical staining, respectively. The results demonstrated that the VSCC‑SCs promoted the differentiation, proliferation, invasion and migration of OSCC cells, while the SCC‑SCs inhibited the differentiation, and promoted the proliferation, invasion and migration of OSCC cells in vivo. Finally, microarray data were used to predict genes in VSCC‑SCs and SCC‑SCs that may influence the progression of OSCC, and those with potential to influence the differential effects of VSCC‑SCs and SCC‑SCs on the differentiation of OSCC. It was found that C‑X‑C motif chemokine ligand (CXCL)8, mitogen‑activated protein kinase 3 (MAPK3), phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit alpha (PIK3CA), C-X-C motif chemokine ligand 1 (CXCL1) and C‑C motif chemokine ligand 2 (CCL2) may be involved in the crosstalk between VSCC‑SCs, SCC‑SCs and OSCC cells, which regulates the progression of OSCC. Intercellular adhesion molecule 1 (ICAM1), interleukin (IL)1B, Fos proto‑oncogene, AP‑1 transcription factor subunit (FOS), bone morphogenetic protein 4 (BMP4), insulin (INS) and nerve growth factor (NGF) may be responsible for the differential effects of VSCC‑SCs and SCC‑SCs on the differentiation of OSCC. On the whole, the present study demonstrates that both VSCC‑SCs and SCC‑SCs may promote the progression of OSCC, and SCC‑SCs were found to exert a more prominent promoting effect; this may represent a potential regulatory mechanism for the progression of OSCC.
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Shan Q, Takabatake K, Omori H, Kawai H, Oo MW, Nakano K, Ibaragi S, Sasaki A and Nagatsuka H: Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma. Int J Oncol 59: 72, 2021.
APA
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M.W., Nakano, K. ... Nagatsuka, H. (2021). Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma. International Journal of Oncology, 59, 72. https://doi.org/10.3892/ijo.2021.5252
MLA
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M. W., Nakano, K., Ibaragi, S., Sasaki, A., Nagatsuka, H."Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma". International Journal of Oncology 59.3 (2021): 72.
Chicago
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M. W., Nakano, K., Ibaragi, S., Sasaki, A., Nagatsuka, H."Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma". International Journal of Oncology 59, no. 3 (2021): 72. https://doi.org/10.3892/ijo.2021.5252
Copy and paste a formatted citation
x
Spandidos Publications style
Shan Q, Takabatake K, Omori H, Kawai H, Oo MW, Nakano K, Ibaragi S, Sasaki A and Nagatsuka H: Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma. Int J Oncol 59: 72, 2021.
APA
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M.W., Nakano, K. ... Nagatsuka, H. (2021). Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma. International Journal of Oncology, 59, 72. https://doi.org/10.3892/ijo.2021.5252
MLA
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M. W., Nakano, K., Ibaragi, S., Sasaki, A., Nagatsuka, H."Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma". International Journal of Oncology 59.3 (2021): 72.
Chicago
Shan, Q., Takabatake, K., Omori, H., Kawai, H., Oo, M. W., Nakano, K., Ibaragi, S., Sasaki, A., Nagatsuka, H."Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma". International Journal of Oncology 59, no. 3 (2021): 72. https://doi.org/10.3892/ijo.2021.5252
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