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Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis

  • Authors:
    • Runze Wang
    • Cuixiu Peng
    • Junying Song
    • Yanan Hua
    • Qinglan Wu
    • Lin Deng
    • Yi Cao
    • Jinyu Zhang
    • Li Zhang
    • Li Wu
    • Lin Hou
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Neurobiology, Basic Medical College, Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266000, P.R. China, Experimental Center for Undergraduates of Pharmacy, School of Pharmacy, Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Breast Center, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong 266000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 33
    |
    Published online on: February 17, 2022
       https://doi.org/10.3892/ijo.2022.5323
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Abstract

Regulator of ribosome synthesis 1 (RRS1) is a key factor in ribosome biosynthesis and other cellular functions. High level of RRS1 in breast cancer cell lines is associated with increased cell proliferation, invasion and migration. RRS1 controls the assembly of the 60s subunit and maturation of 25S rRNA during ribosome biosynthesis. In this study, lentiviral transfection of sh‑RNA was used to knock down the level of RRS1, to detect the effect of RRS1 on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis by COIP and dual‑luciferase reporter gene assays. The present study found that RRS1 knockdown reduced the accumulation of ribosome protein L11 (RPL11) in the nucleolus, which then migrated to the nucleoplasm and bound to c‑Myc. This inhibited trans‑activation of SNAIL by c‑Myc and eventually decreased the invasion and metastasis capacity of the human breast cancer cell line BT549. Taken together, RRS1 regulates invasion and metastasis of human breast cancer cells through the RPL11‑c‑Myc‑SNAIL axis. The findings are of great significance for exploring the mechanism of breast cancer invasion and metastasis and the corresponding regulatory factors.
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Spandidos Publications style
Wang R, Peng C, Song J, Hua Y, Wu Q, Deng L, Cao Y, Zhang J, Zhang L, Wu L, Wu L, et al: Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. Int J Oncol 60: 33, 2022.
APA
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L. ... Hou, L. (2022). Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. International Journal of Oncology, 60, 33. https://doi.org/10.3892/ijo.2022.5323
MLA
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L., Cao, Y., Zhang, J., Zhang, L., Wu, L., Hou, L."Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis". International Journal of Oncology 60.3 (2022): 33.
Chicago
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L., Cao, Y., Zhang, J., Zhang, L., Wu, L., Hou, L."Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis". International Journal of Oncology 60, no. 3 (2022): 33. https://doi.org/10.3892/ijo.2022.5323
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Spandidos Publications style
Wang R, Peng C, Song J, Hua Y, Wu Q, Deng L, Cao Y, Zhang J, Zhang L, Wu L, Wu L, et al: Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. Int J Oncol 60: 33, 2022.
APA
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L. ... Hou, L. (2022). Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. International Journal of Oncology, 60, 33. https://doi.org/10.3892/ijo.2022.5323
MLA
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L., Cao, Y., Zhang, J., Zhang, L., Wu, L., Hou, L."Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis". International Journal of Oncology 60.3 (2022): 33.
Chicago
Wang, R., Peng, C., Song, J., Hua, Y., Wu, Q., Deng, L., Cao, Y., Zhang, J., Zhang, L., Wu, L., Hou, L."Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis". International Journal of Oncology 60, no. 3 (2022): 33. https://doi.org/10.3892/ijo.2022.5323
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